Genes de virulencia y bacteriocinas en cepas de Enterococcus faecalis aisladas desde diferentes muestras clínicas en la Región del Maule, Chile

E, Carlos Padilla; A, María Núñez; G, Andrés Padilla; G, Olga Lobos

doi:10.4067/s0716-10182012000100010

Cited by 8 publications

(5 citation statements)

References 28 publications

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“…Many studies with clinical strains of E . faecalis have reported the expression of efa , gelE , asa , cyl in the isolates [63, 64]. Genes involved in adhesion and antibiotic resistances were found to be overexpressed in biofilm bacteria [65] as observed in our system.…”

Section: Discussionsupporting

confidence: 55%

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

et al. 2017

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Propolis obtained from bee hives is a natural substance with antimicrobial properties. It is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of Malaysian propolis were prepared. Both the extracts displayed antimicrobial and anti-biofilm properties against Enterococcus faecalis, a common bacterium associated with hospital-acquired infections. High performance liquid chromatography (HPLC) analysis of propolis revealed the presence of flavonoids like kaempferol and pinocembrin. This study investigated the role of propolis developed into nanoparticles with chitosan for its antimicrobial and anti-biofilm properties against E. faecalis. Bacteria that grow in a slimy layer of biofilm are resistant to penetration by antibacterial agents. The use of nanoparticles in medicine has received attention recently due to better bioavailability, enhanced penetrative capacity and improved efficacy. A chitosan-propolis nanoformulation was chosen based on ideal physicochemical properties such as particle size, zeta potential, polydispersity index, encapsulation efficiency and the rate of release of the active ingredients. This formulation inhibited E. faecalis biofilm formation and reduced the number of bacteria in the biofilm by ~90% at 200 μg/ml concentration. When tested on pre-formed biofilms, the formulation reduced bacterial number in the biofilm by ~40% and ~75% at 200 and 300 μg/ml, respectively. The formulation not only reduced bacterial numbers, but also physically disrupted the biofilm structure as observed by scanning electron microscopy. Treatment of biofilms with chitosan-propolis nanoparticles altered the expression of biofilm-associated genes in E. faecalis. The results of this study revealed that chitosan-propolis nanoformulation can be deemed as a potential anti-biofilm agent in resisting infections involving biofilm formation like chronic wounds and surgical site infections.

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Section: Discussionsupporting

confidence: 55%

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

et al. 2017

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“…Recently, Padilla et al (2012) studied the presence of some bacteriocin determinants in clinical E. faecalis strains isolated in Chile during the period 2008-2009. Contrary to our results, they found significantly higher frequency for entlA (31.3 vs 19.7 %, p < 0.01) and as-48 (9.3 % / 0.7 %, p < 0.02).…”

Section: Discussionmentioning

confidence: 99%

Molecular genetic study of potentially bacteriocinogenic clinical and dairy Enterococcus spp. isolates from Bulgaria

et al. 2015

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A total of 424 Enterococcus spp. isolates from clinical samples (n =398) and traditional Bulgarian artisanal cheeses (n=26) were studied in order to evaluate the incidence and expression of bacteriocin genetic determinants. Structural genes coding for enterocin A (entA), enterocin P (entP), enterocin B (entB), enterocin AS-48 (as-48) and enterolysin A (entlA) were detected by polymerase chain reaction (PCR), while reverse transcription PCR was performed to establish the gene expression. Of the total isolates, 163 (38.4 %) were potentially bacteriocinogenic. The genes typical of Enterococcus faecium (entA, entB and entP) were found in 84.0, 8.4 and 15.1 % of the E. faecium isolates, respectively. Focusing on E. faecalis isolates, the frequencies of entlA and as-48 were 19.3 and 0.7 %, respectively. No significant differences in the gene presence between clinical and dairy isolates were ascertained. Bacteriocin genes were found to be expressed at transcriptional level in 83.4 % of the bacteriocinogenic Enterococcus spp. isolates: a single gene in 113 samples (69.3 %), two genes in 21 (12.9 %) and three genes in 2 isolates (1.2 %). The present investigation may serve as a basis for further studies for elucidating the spread of the bacteriocin genetic determinants in the two enterococcal populations (clinical and dairy).

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“…Recently Padilla et al published their data on incidence of virulence genes amongst strains of E. faecalis isolated from UTIs and bacteremia in Chile, for the period of 2008–2009. 35 The rates for the following genes from urine samples in our study were significantly higher than those reported in Chile: efaA (94.3%/62%, p < 0.001), eep (98.9%/72%, p < 0.001), gelE (83%/28%, p < 0.001), cylA (62.5%/40%, p < 0.01); while the presence of agg was significantly lower (43.2%/78%, p < 0.001). Our isolates from bacteremia showed a significantly higher frequency of efaA compared to the Chilean isolates (66.7%/28%, p < 0.001).…”

Section: Discussionmentioning

confidence: 99%

Incidence of virulence determinants in clinical Enterococcus faecalis and Enterococcus faecium isolates collected in Bulgaria

Strateva

Atanasova

Savov

et al. 2016

The Brazilian Journal of Infectious Diseases

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Most E. faecalis attaches to abiotic surfaces in hospital environment, which correlates with higher prevalence of gene encoding for virulence factors involved in biofilm formation, such as enterococcal surface protein, aggregation substance, and gelatinase. The intestinal tract is an important reservoir for opportunistic enterococcal pathogens and allows them to access infectious sites through different virulence factors, demonstrated in outpatient isolates in this study.

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Genes de virulencia y bacteriocinas en cepas de Enterococcus faecalis aisladas desde diferentes muestras clínicas en la Región del Maule, Chile

Cited by 8 publications

References 28 publications

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Molecular genetic study of potentially bacteriocinogenic clinical and dairy Enterococcus spp. isolates from Bulgaria

Incidence of virulence determinants in clinical Enterococcus faecalis and Enterococcus faecium isolates collected in Bulgaria

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