Genes for NUE in rice: a way forward for molecular breeding and genome editing

Neeraja, C. N.; Barbadikar, Kalyani M.; Mangrauthia, Satendra K.; Rao, P. Raghuveer; Subrahmanayam, Desiraju; Sundaram, R. M.

doi:10.1007/s40502-021-00632-x

Cited by 10 publications

(2 citation statements)

References 122 publications

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“…miR5640 was confirmed to target phosphoenolpyruvate carboxylase (PEPC) which is vital in maintaining C/N balance [77]. Other transcription factors such as NLP7, Dof1, GATA were also found to play important roles in regulating nitrogen assimilation process [72,78]. An approach using methionine sulphoximine to inhibit glutamine synthetase and prevent ammonium assimilation identified many genes responding to downstream reduced nitrogen-containing compounds rather than nitrate as previously assumed [79].…”

Section: Genetic Basis Of Nitrogen Assimilation and Remobilizationmentioning

confidence: 78%

“…A number of transcription factors (TF) that regulate N transporters also play important roles in N uptake, such as MADS-box TF ANR1, LOB Domain-Containing proteins (LBD37/38/39), Teosinte branched 1/Cycloidea/Proliferatingcell Factor 1-20 (TCP20), NIN-like proteins (NLP6, NLP7), Nitrate regulatory gene 2 (NRG2), Nuclear factor Y (NF-YA), High nitrogen insensitive 9 (HNI9), Hypersensitivity to Low Pi-Elicited Primary Root Shortening 1 (HRS1), to name a few (reviewed by Plett et al [71]). It was also reported that some of the transcription factors or genes are induced by nitrogen starvation condition, such as basic leucine zipper (bZIP) transcription factor gene, AtTGA4, cytokinin synthesis gene isopentenyltransferase (IPT) [72]. Manipulating those genes through genetic approach can potentially improve nitrogen uptake.…”

Section: Genetic Regulation Of Nitrogen Uptakementioning

confidence: 99%

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Current Progress and Future Prospect of Wheat Genetics Research towards an Enhanced Nitrogen Use Efficiency

Zhao

Islam

Alhabbar

et al. 2023

Plants

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To improve the yield and quality of wheat is of great importance for food security worldwide. One of the most effective and significant approaches to achieve this goal is to enhance the nitrogen use efficiency (NUE) in wheat. In this review, a comprehensive understanding of the factors involved in the process of the wheat nitrogen uptake, assimilation and remobilization of nitrogen in wheat were introduced. An appropriate definition of NUE is vital prior to its precise evaluation for the following gene identification and breeding process. Apart from grain yield (GY) and grain protein content (GPC), the commonly recognized major indicators of NUE, grain protein deviation (GPD) could also be considered as a potential trait for NUE evaluation. As a complex quantitative trait, NUE is affected by transporter proteins, kinases, transcription factors (TFs) and micro RNAs (miRNAs), which participate in the nitrogen uptake process, as well as key enzymes, circadian regulators, cross-talks between carbon metabolism, which are associated with nitrogen assimilation and remobilization. A series of quantitative genetic loci (QTLs) and linking markers were compiled in the hope to help discover more efficient and useful genetic resources for breeding program. For future NUE improvement, an exploration for other criteria during selection process that incorporates morphological, physiological and biochemical traits is needed. Applying new technologies from phenomics will allow high-throughput NUE phenotyping and accelerate the breeding process. A combination of multi-omics techniques and the previously verified QTLs and molecular markers will facilitate the NUE QTL-mapping and novel gene identification.

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Section: Genetic Basis Of Nitrogen Assimilation and Remobilizationmentioning

confidence: 78%

Section: Genetic Regulation Of Nitrogen Uptakementioning

confidence: 99%

Current Progress and Future Prospect of Wheat Genetics Research towards an Enhanced Nitrogen Use Efficiency

Zhao

Islam

Alhabbar

et al. 2023

Plants

View full text Add to dashboard Cite

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Nitrate-responsive transcriptome analysis of rice RGA1 mutant reveals the role of G-protein alpha subunit in negative regulation of nitrogen-sensitivity and use efficiency

Prasanna,

Mandal,

Kumar

et al. 2023

Plant Cell Rep

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Nitrogen (N) use e ciency (NUE) is important for sustainable agriculture. G-protein signalling was implicated in N-response/NUE in rice, but needs genetic characterization of the role of α subunit (RGA1). We found that RGA1 knock-out mutant in japonica rice exhibited lesser nitrate-dose sensitivity, higher yield and NUE relative to the wild type (WT). We investigated its genomewide basis using nitrateresponsive rga1 transcriptome relative to WT. It revealed 3,416 differentially expressed genes (DEGs), including 719 associated with development, grain yield and phenotypic traits for NUE. The upregulated DEGs were related to photosynthesis, chlorophyll, tetrapyrrole and porphyrin biosynthesis, while the downregulated DEGs belonged to cellular protein metabolism and transport, small GTPase signalling, cell redox homeostasis etc. RT-qPCR validated 26 nitrate-responsive DEGs across functional categories. Physiological validation of nitrate-response in the mutant and WT at 1.5 and 15 mM doses revealed higher chlorophyll and stomatal length but decreased stomatal density, conductance and transpiration.The consequent increase in photosynthesis and water use e ciency may contribute to higher yield and NUE in the mutant, whereas the WT was N-dose sensitive. The mutant was not as N dose-responsive as WT in shoot/root growth, productive tillers and heading date, but equally responsive as WT in total N and protein content. RGA1mutant is less impacted by higher N-dose or salt stress in terms of yield, protein content, photosynthetic performance, relative water content, water use e ciency and catalase activity. PPI-network analyses revealed known NUE-related proteins as RGA1 interactors. Therefore, RGA1 regulates N-dose sensitivity and NUE in rice. Key MessageNitrate-responsive transcriptomic, phenotypic and physiological analyses of rice RGA1 mutant revealed many novel RGA1-regulated genes/processes/traits related to nitrogen use e ciency, and provided robust genetic evidence of RGA1-regulation of NUE.

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Expression levels of nitrogen assimilation-related genes, physiological responses, and morphological adaptations of three indica rice (Oryza sativa L. ssp. indica) genotypes subjected to nitrogen starvation conditions

et al. 2022

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Genes for NUE in rice: a way forward for molecular breeding and genome editing

Cited by 10 publications

References 122 publications

Current Progress and Future Prospect of Wheat Genetics Research towards an Enhanced Nitrogen Use Efficiency

Current Progress and Future Prospect of Wheat Genetics Research towards an Enhanced Nitrogen Use Efficiency

Nitrate-responsive transcriptome analysis of rice RGA1 mutant reveals the role of G-protein alpha subunit in negative regulation of nitrogen-sensitivity and use efficiency

Expression levels of nitrogen assimilation-related genes, physiological responses, and morphological adaptations of three indica rice (Oryza sativa L. ssp. indica) genotypes subjected to nitrogen starvation conditions

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