Genes Highly Expressed in the Early Phase of Murine Graft-versus-Host Reaction

Wakui, Masatoshi; Yamaguchi, Akihiro; Sakurai, Daiju; Ogasawara, Kunio; Yokochi, Taeko; Tsuchiya, Naoyuki; Ikeda, Yasuo; Tokunaga, Katsushi

doi:10.1006/bbrc.2001.4550

Cited by 5 publications

(2 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Overall, these markers exhibited expected and consistent expression patterns in that higher expression occurred in zone I and lower expression occurred in zone III. Zone II markers showed less consistency in both the amplitude of the signal intensity and the actual pattern of gene expression [4], [36]–[40].…”

Section: Resultsmentioning

confidence: 98%

See 1 more Smart Citation

Genome-Wide Analyses of Gene Expression during Mouse Endochondral Ossification

et al. 2010

View full text Add to dashboard Cite

BackgroundEndochondral ossification is a complex process involving a series of events that are initiated by the establishment of a chondrogenic template and culminate in its replacement through the coordinated activity of osteoblasts, osteoclasts and endothelial cells. Comprehensive analyses of in vivo gene expression profiles during these processes are essential to obtain a complete understanding of the regulatory mechanisms involved.Methodology/Principal FindingsTo address these issues, we completed a microarray screen of three zones derived from manually segmented embryonic mouse tibiae. Classification of genes differentially expressed between each respective zone, functional categorization as well as characterization of gene expression patterns, cytogenetic loci, signaling pathways and functional motifs both confirmed reported data and provided novel insights into endochondral ossification. Parallel comparisons of the microdissected tibiae data set with our previously completed micromass culture screen further corroborated the suitability of micromass cultures for modeling gene expression in chondrocyte development. The micromass culture system demonstrated striking similarities to the in vivo microdissected tibiae screen; however, the micromass system was unable to accurately distinguish gene expression differences in the hypertrophic and mineralized zones of the tibia.Conclusions/SignificanceThese studies allow us to better understand gene expression patterns in the growth plate and endochondral bones and provide an important technical resource for comparison of gene expression in diseased or experimentally-manipulated cartilages. Ultimately, this work will help to define the genomic context in which genes are expressed in long bones and to understand physiological and pathological ossification.

show abstract

Section: Resultsmentioning

confidence: 98%

“…Chondrogenesis is typified by the expression of Sox transcription factors 5,6 and 9 [1], [4]. Proliferating chondrocytes synthesize an ECM composed mainly of collagen II and aggrecan, among others, while the central ECM molecule expressed in hypertrophic cartilage is collagen X [5].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analyses of Gene Expression during Mouse Endochondral Ossification

et al. 2010

View full text Add to dashboard Cite

show abstract

Autonomous Biomolecular Computer Modeled after Retroviral Replication

Nitta

Suyama

2004

DNA Computing

View full text Add to dashboard Cite

Vitamin C and E supplementation prevents mitochondrial damage of ileum myocytes caused by intense and exhaustive exercise training

Rosa

Ribeiro

Pereira

et al. 2009

Journal of Applied Physiology

View full text Add to dashboard Cite

Intense and exhaustive exercise (IEE) is associated with oxidative stress in skeletal muscle, and we recently reported that intestine is sensitive to IEE. In the present study, we investigated the possible relationship between the effects of IEE on morphology and oxidative markers in the ileum and isolated mitochondria. C57BL/6 mice were ascribed either to a control group comprising two subgroups, one sedentary and another exercised for 10 days (E10), or to a corresponding supplemented control group again comprising two subgroups, one sedentary and another exercised for 10 days (E10-V). The IEE program consisted of a single daily treadmill running session at 85% of V(max), until animal exhaustion. Vitamins C (10 mg/kg) and E (10 mg/kg) were concurrently intraperitoneally administered 2 h before the exercise sessions. IEE was shown to cause 1) impairment of ileum internal membrane mitochondria verified by ultramicrography analysis; 2) increase in ileum carbonyl content (117%) and reduction in antioxidant capacity (36%); 3) increase in mitochondria carbonyl content (38%), increase in the percentage of ruptured mitochondria (25.3%), increase in superoxide dismutase activity (186%), and reduction in citrate synthase activity (40.4%) compared with control animals. Observations in the vitamin-supplemented exercised animals (E10-V) were 1) healthy appearance of myocyte mitochondria; 2) decrease in ileum carbonyl content (66%) and increase in antioxidant capacity (53%); 3) decrease in mitochondria carbonyl content (43%), decrease in the percentage of ruptured mitochondria (30%), slight increase in superoxide dismutase activity (7%), and significant increase in citrate synthase activity (121%) compared with E10 animals. Therefore, the present results strongly corroborate the hypothesis that IEE leads to marked disturbances in intestinal mitochondria, mainly in redox status, and affects whole intestinal redox status.

show abstract

Genes Highly Expressed in the Early Phase of Murine Graft-versus-Host Reaction

Cited by 5 publications

References 53 publications

Genome-Wide Analyses of Gene Expression during Mouse Endochondral Ossification

Genome-Wide Analyses of Gene Expression during Mouse Endochondral Ossification

Autonomous Biomolecular Computer Modeled after Retroviral Replication

Vitamin C and E supplementation prevents mitochondrial damage of ileum myocytes caused by intense and exhaustive exercise training

Contact Info

Product

Resources

About