Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens

Kalica, Anthony R.; Greenberg, Harry B.; Wyatt, Richard G.; Flores, Jorge; Sereno, Mitzi M.; Kapikian, Albert Z.; Chanock, Robert M.

doi:10.1016/0042-6822(81)90285-3

Cited by 276 publications

(193 citation statements)

References 9 publications

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“…Vice versa, it was found, that viruses of different species belonging to the L electropherotype differed in their subgroup (Thouless, Beards & Flewett, 1982). This is conceivable, as the antigen defining subgroup specificity is coded for by RNA segment 6 Kalica et al 1981 b) so that mutational changes (or reassortment) in only this segment could affect subgroup specificity. An analogous statement can be made for serotypes whose specificity is dependent on the protein product of segment 9 (Both, Mattick & Bellamy, 1983).…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Follett

Sanders

Beards

et al. 1984

J. Hyg.

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SUMMARYThe molecular epidemiology of rotavirus infections in Glasgow and the west of Scotland during 1981/82 and 1982/83 was investigated by electron microscopy, ELISA testing and RNA migration pattern analysis. In 1981/82, rotaviruses of both the 'long' and the 'short 'electropherotype (in different variants) co-circulated from the onset throughout the winter peak of the outbreak. Approximately 80 o of the children were infected during the first year of life. No differences in incidence were found between sexes. In 1982/83 the isolated rotaviruses were almost exclusively of the 'long' electropherotype (in different variants) and 360 of the children were infected beyond the first year of life. Rotaviruses of the 'long' electropherotype serologically were of subgroup ii and serotype 1 and those of the 'short' electropherotype of subgroup i and serotype 2.

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Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Follett

Sanders

Beards

et al. 1984

J. Hyg.

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“…Rotavirus particles consist of an outer and inner capsid and contain 11 segments of genomic RNA. Two major neutralization proteins on the outer capsid, VP4 and VP7, are encoded respectively by RNA segment 4 (Mason et al, 1983;Kalica et al, 1983) and segments 7, 8 or 9 depending on the strain (Greenberg et al, 1983a;Kalica et al, 1981;Ward et al, 1988). Most of the serotype-specific neutralization epitopes are located on VP7 (Greenberg et al, 1983bTaniguchi et al, 1985), while common antigens among different serotypes are mainly on VP4 (Mackow et aL, 1988b;Taniguchi et al, 1988b).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Newly Identified Neutralization Epitopes on VP7 of Human Rotavirus Serotype 1

Kobayashi

Taniguchi²,

Urasawa³

1991

Journal of General Virology

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Neutralizing monoclonal antibodies (MAbs) directed to the VP7 protein and neutralization-resistant mutants were used to analyse the antigenic structure of VP7 of human rotavirus serotype 1. Cross-neutralization tests using the MAbs and the resistant mutants indicated the existence of two functionally independent neutralization epitope regions (S1 and $2) on VP7. Region S1 corresponds to a single epitope domain of VP7 which has been detected previously. Two MAbs prepared in this study recognized the S1 region, and the resistant mutants they selected had amino acid substitutions at positions 94 or 213. On the other hand, region $2 is considered to be a novel epitope. Single or double amino acid substitutions were detected in the variable regions (amino acid positions 145, 217 and 221) and in the constant regions (positions 104, 201 and 291) of the VP7 protein of mutants selected by MAbs directed to the $2 region. It was suggested that the variable region E (amino acids 208 to 221) includes two independent neutralization sites, and that amino acid substitutions in the constant region of VP7 also affect serotype-specific neutralization epitopes. Neutralization epitopes on VP7 are considered to be highly dependent on the conformation of VP7.

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“…In rotaviruses, the two outer capsid proteins, VP4 and VP7, have been shown to be the neutralizable antigens (Kalica et al, 1981 ;Greenberg et al, 1983). However, no studies have been conducted in order to identify the neutralizing antigens in aquareoviruses.…”

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confidence: 99%

Cloning, sequence analysis and expression of the major outer capsid protein gene of an aquareovirus.

Lupiani¹,

Reddy

Subramanian

et al. 1997

Journal of General Virology

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The nucleotide and deduced amino acid sequences of genome segment 10 of aquareovirus strain SBR, encoding the major outer capsid protein (VP7), have been determined. Genome segment 10 of SBR virus is 986 nucleotides long and encodes a polypeptide of 298 amino acids with a predicted molecular mass of 32 430 Da. There are 26 nontranslated nucleotides at the 5h end and 66 nontranslated nucleotides at the 3h end. Using a recombinant baculovirus system, the VP7 protein of SBR virus was expressed to a high level. The baculovirus-produced VP7 protein was similar both in its size and antigenic properties to the authentic aquareovirus VP7 protein. Antiserum from a rabbit immunized with the baculovirus-produced VP7 protein failed to neutralize the homologous aquareovirus strain. As determined by Western blotting, this antiserum reacted with aquareovirus strains belonging to the same genogroup as SBR virus, but did not react with aquareovirus strains belonging to the other genogroups.

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Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens

Cited by 276 publications

References 9 publications

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Analysis of the Newly Identified Neutralization Epitopes on VP7 of Human Rotavirus Serotype 1

Cloning, sequence analysis and expression of the major outer capsid protein gene of an aquareovirus.

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