Genes that control neuromuscular specificity in Drosophila

Vactor, David Van; Sink, Helen; Fambrough, Douglas M.; Tsoo, Rosalie; Goodman, Corey S.

doi:10.1016/0092-8674(93)90643-5

Cited by 401 publications

(296 citation statements)

References 52 publications

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“…The mAbs (culture media) that recognize subsets of neurons in the embryonic CNS and peripheral nervous system (PNS) were obtained from the Developmental Studies Hybridoma Bank at the University of Iowa (Iowa City) and were used as follows: mAb 22C10 (anti-futsch), 1:100 dilution, for both CNS and PNS neuron and axon subsets (15); mAb BP102, 1:100 dilution, to label some CNS axons (16); and mAb 1D4 (anti-FasII), 1:100 dilution, to stain some motor neurons and their axons (17). Ab-antigen complexes were detected by using biotinylated anti-mouse IgG (Vector Laboratories) and the Vectastain Elite ABC and DAB substrate kits (Vector Laboratories).…”

Section: Methodsmentioning

confidence: 99%

Genes required for Drosophila nervous system development identified by RNA interference

Ivanov

Rovescalli

Pozzi

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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RNA interference was used to screen 3,314 Drosophila doublestranded RNAs, corresponding to Ϸ25% of Drosophila genes, for genes that affect the development of the embryonic nervous system. RNA-interference-mediated gene silencing in Drosophila embryos resulted in loss-of-function mutant phenotypes for 43 genes, which is 1.3% of the genes that were screened. We found 18 genes that were not known previously to affect the development of the nervous system. The functions of some of the genes are unknown. Other genes encode protein kinases, transcription factors, and signaling proteins, as well as proteins with other functions.embryonic nervous system ͉ neural mutants ͉ neural development

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Section: Methodsmentioning

confidence: 99%

Genes required for Drosophila nervous system development identified by RNA interference

Ivanov

Rovescalli

Pozzi

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…Embryos were prepared and stained with antibodies as previously described (12). Antibodies were used at the following concentrations: mAb 1D4 anti-Fasciclin II, 1:10; mAb 3C10 anti-Even-skipped, 1:2; mAb BP102, 1:5; mAb 7G10 anti-Fasciclin III, 1:20. kuz mutations were introduced in a FasIII mutant background (11,12), and mAb 7G10 was used to identify homozygous mutant embryos. The phenotypes described in this paper do not depend on the absence of Fasciclin III.…”

Section: Methodsmentioning

confidence: 99%

“…To determine which genes are necessary for proper axon growth and targeting in the Drosophila embryo, we have recently undertaken genetic screens to look for mutations which disrupt the development of the central nervous system (CNS) and motor axon pathways (11,12). These screens have identified several genes required for axon guidance and targeting (13)(14)(15).…”

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confidence: 99%

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The cell surface metalloprotease/disintegrin Kuzbanian is required for axonal extension in Drosophila

Fambrough

Pan

Rubin

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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172

105

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It has long been suspected that proteolytic activity associated with advancing growth cones may be required for axon extension. We have isolated mutations in the kuzbanian (kuz) gene, which is expressed in the nervous system and encodes a putative zinc metalloprotease with a disintegrin domain. Drosophila embryos with loss-of-function mutations in kuz have dramatic defects in the development of central nervous system axon pathways, with many axons stalling and failing to extend through the nerve cord. This phenotype is rescued by panneural expression of kuz mRNA in the embryo. These results show that the Kuz metalloprotease is required for axon extension, suggesting a requirement for proteolytic activity at the growth cone surface.

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“…In situ hybridization was performed essentially as described previously (Tautz and Pfeifle, 1989), with slight modification (I. Duncan, personal communication). The following antibodies and concentrations were used for protein localization: mAbBP102 (1:10; Klambt and Goodman, 1991); mouse anti-Fasciclin II (1:10; Vactor et al, 1993); rat anti-RK2 (1:1,000; Campbell et al, 1994); mouse anti-engrailed (1:10; Patel et al, 1989); and mouse anti-eve (1:10; Brown et al, 1997). Immunocytochemistry and ␤-galactosidase staining were performed as described elsewhere (Rushton et al, 1995;Boquet et al, 2000).…”

Section: Mrna and Protein Localizationmentioning

confidence: 99%

Drosophila NAB (dNAB) is an orphan transcriptional co‐repressor required for correct CNS and eye development

Clements

Duncan

Milbrandt

2002

Developmental Dynamics

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The mammalian NAB proteins have been identified previously as potent co-repressors of the EGR family of zinc finger transcription factors. Drosophila NAB (dNAB), like its mammalian counterparts, binds EGR1 and represses EGR1-mediated transcriptional activation from a synthetic promoter. In contrast, dNAB does not bind the Drosophila EGR-related protein klumpfuss. dnab RNA is expressed exclusively in a subset of neuroblasts in the embryonic and larval central nervous system (CNS), as well as in several larval imaginal disc tissues. Here, we describe the creation of targeted deletion mutations in the dnab gene and the identification of additional, EMS-induced dnab mutations by genetic complementation analysis. Null alleles in dnab cause larval locomotion defects and early larval lethality (L1-L2). A putative hypomorphic allele in dnab instead causes early adult lethality due to severe locomotion defects. In the dnab -/-CNS, axon outgrowth/guidance and glial development appear normal; however, a subset of eve؉ neurons forms in reduced numbers. In addition, mosaic analysis in the eye reveals that dnab -/-clones are either very small or absent. Similarly, dNAB overexpression in the eye causes eyes to be very small with few ommatidia. These dramatic eye-specific phenotypes will prove useful for enhancer/suppressor screens to identify dnab-interacting genes. Developmental Dynamics 226:67-81, 2003.

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Genes that control neuromuscular specificity in Drosophila

Cited by 401 publications

References 52 publications

Genes required for Drosophila nervous system development identified by RNA interference

Genes required for Drosophila nervous system development identified by RNA interference

The cell surface metalloprotease/disintegrin Kuzbanian is required for axonal extension in Drosophila

Drosophila NAB (dNAB) is an orphan transcriptional co‐repressor required for correct CNS and eye development

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