Genetic alterations in peritoneal metastatic tumors predicted the outcomes for hyperthermic intraperitoneal chemotherapy

Nguyen, Quynh-Anh; Chou, Wan Hsuan; Hsieh, Mao‐Chih; Chang, Che‐Mai; Luo, Wei-Tzu; Tai, Yu‐Ting; Chang, Wei Chiao

doi:10.3389/fonc.2023.1054406

Cited by 1 publication

(1 citation statement)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cytotoxicity of the P1, P2, and P3 samples was evaluated for the AGP01 lineage, which originates from peritoneal metastasis and has genetic alterations capable of providing more resistance to the action of substances or chemotherapy [ 58 , 59 , 60 ]. As observed by the MTT assay, all piperine samples are cytotoxic against the tested model, with IC50 values of 16.81 µg/mL (P1), 12.06 µg/mL (P2), and 16.69 µg/mL (P3), as shown in Figure 8 .…”

Section: Resultsmentioning

confidence: 99%

Extraction, Characterization, and Evaluation of the Cytotoxic Activity of Piperine in Its Isolated form and in Combination with Chemotherapeutics against Gastric Cancer

et al. 2023

View full text Add to dashboard Cite

Gastric cancer is one of the most frequent types of neoplasms worldwide, usually presenting as aggressive and difficult-to-manage tumors. The search for new structures with anticancer potential encompasses a vast research field in which natural products arise as promising alternatives. In this scenario, piperine, an alkaloid of the Piper species, has received attention due to its biological activity, including anticancer attributes. The present work proposes three heating-independent, reliable, low-cost, and selective methods for obtaining piperine from Piper nigrum L. (Black pepper). Electronic (SEM) and optical microscopies, X-ray diffraction, nuclear magnetic resonance spectroscopies (13C and 1H NMR), and optical spectroscopies (UV–Vis, photoluminescence, and FTIR) confirm the obtention of piperine crystals. The MTT assay reveals that the piperine samples exhibit good cytotoxic activity against primary and metastasis models of gastric cancer cell lines from the Brazilian Amazon. The samples showed selective cytotoxicity on the evaluated models, revealing higher effectiveness in cells bearing a higher degree of aggressiveness. Moreover, the investigated piperine crystals demonstrated the ability to act as a good cytotoxicity enhancer when combined with traditional chemotherapeutics (5-FU and GEM), allowing the drugs to achieve the same cytotoxic effect in cells employing lower concentrations. These results establish piperine as a promising molecule for therapy investigations in aggressive gastric cancer, both in its isolated form or as a bioenhancer.

show abstract

Section: Resultsmentioning

confidence: 99%