Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution

Abstract

BackgroundThe entomopathogenic anamorphic fungus Beauveria bassiana is currently used as a biocontrol agent (BCA) of insects. Fifty-seven Beauveria bassiana isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of B. bassiana.ResultsGroup I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the B. bassiana s.s. (sensu stricto) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found.ConclusionMost Spanish B. bassiana isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish B. bassiana isolates were sampled.

Lethal effects of a Mexican Beauveria bassiana (Balsamo) strain against Meccus pallidipennis (Stal)

“…However, they have also shown that these approaches are insufficient to investigate species such as B. bassiana (Rehner and Buckley, 2005). Phylogenetic analysis of the sequences of EF1-a resulted in cladograms with higher resolution, consistent with the results of Rehner and Buckley (2005) and Garrido-Jurado et al (2011). The resulting topologies of phylogenetic analyses of sequences of the ITS and EF1-α genes separately, were highly similar to the topologies obtained by Rehner and Buckley (2005).…”

“…Species recognition in Beauveria is difficult due to a lack of taxonomically informative morphology. A molecular phylogenetic analysis, based on nuclear ribosomal internal transcribed spacer (ITS) and elongation factor 1-alpha (EF1-α) sequences for isolates from diverse geographic origins, habitats and insect hosts, was used to resolve six well supported clades within Beauveria bassiana Vuillemin (Rehner and Buckley, 2005; Garrido-Jurado et al ., 2011). …”

Diversity of entomopathogenic Hypocreales in soil and phylloplanes of five Mediterranean cropping systems

“…Studies on the genetic diversity of entomopathogenic Hypocreales have typically been performed using isolates from culture collections (Garrido-Jurado et al, 2011), by infecting a certain pest (Aquino de Muro et al, 2005;Rehner et al, 2006), by focusing on one habitat (Hollingsworth et al, 2011;Schneider et al, 2012;Inglis et al, 2008;Meyling and Eilenberg, 2006a,b), or by collecting specimens from regions worldwide (Freed et al, 2011). None of these studies has simultaneously increased the understanding of species ecology on a local scale and in habitats in different ecosystems (Meyling and Eilenberg, 2007).…”

Cepa nativa de Beauveria pseudobassiana como agente potencial en el control biológico de Monochamus galloprovincialis, vector del nematodo de la madera del pino

“…El gen nuclear EF-1α fue amplificado, secuenciado y analizado en las diferentes muestras. Un fragmento de 1100 pares de bases de dicho gen se amplificó con los primers tef1fw (O'Donnell et al, 1998) y 1750-R (Garrido-Jurado et al, 2011. Las alineaciones se generaron utilizando el programa MegAlign (DNASTAR, 1989-92, Londres, Reino Unido).…”