Genetic analysis and molecular resistance to race 2 of Fusarium wilt in pigeonpea [ Cajanus cajan (L.) Millsp.]

Patil, Prakash; Dubey, Jyotirmay; Saabale, P. R.; Mishra, Raj Kumar; Pandey, Gaurav; Das, Alok; Rathore, Meenal; Singh, Farindra; Singh, Narendra Pratap

doi:10.1016/j.cropro.2017.06.016

Cited by 11 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In pigeonpea, different research groups have found significant MTAs and candidate genes for FW by using SSR (Patil et al, 2017a) and SNP markers Saxena et al, 2017).…”

Section: Identification Of Resistance Loci and Molecular Marker For Fmentioning

confidence: 99%

Breeding, Genetics, and Genomics Approaches for Improving Fusarium Wilt Resistance in Major Grain Legumes

et al. 2020

Self Cite

View full text Add to dashboard Cite

Fusarium wilt (FW) disease is the key constraint to grain legume production worldwide. The projected climate change is likely to exacerbate the current scenario. Of the various plant protection measures, genetic improvement of the disease resistance of crop cultivars remains the most economic, straightforward and environmental-friendly option to mitigate the risk. We begin with a brief recap of the classical genetic efforts that provided first insights into the genetic determinants controlling plant response to different races of FW pathogen in grain legumes. Subsequent technological breakthroughs like sequencing technologies have enhanced our understanding of the genetic basis of both plant resistance and pathogenicity. We present noteworthy examples of targeted improvement of plant resistance using genomics-assisted approaches. In parallel, modern functional genomic tools like RNA-seq are playing a greater role in illuminating the various aspects of plant-pathogen interaction. Further, proteomics and metabolomics have also been leveraged in recent years to reveal molecular players and various signaling pathways and complex networks participating in host-pathogen interaction. Finally, we present a perspective on the challenges and limitations of highthroughput phenotyping and emerging breeding approaches to expeditiously develop FW-resistant cultivars under the changing climate.

show abstract

“…In pigeonpea, different research groups have found significant MTAs and candidate genes for FW by using SSR (Patil et al, 2017a) and SNP markers Saxena et al, 2017).…”

Section: Identification Of Resistance Loci and Molecular Marker For Fmentioning

confidence: 99%

Breeding, Genetics, and Genomics Approaches for Improving Fusarium Wilt Resistance in Major Grain Legumes

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genetic variability among F. udum isolates from various locations of India and Kenya were identified through DNA-based sensitive and precise methods like randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) by several researchers 5 , 7 , 8 . Additionally, out crossing nature, long life cycle and difficulty in accurate phenotyping were other obstacles of conventional resistance breeding efforts 9 , 10 . Genetic improvement of pigeonpea was limited due to inadequate genomic resources and low level of genetic diversity in the primary gene pool 2 , 11 .…”

Section: Introductionmentioning

confidence: 99%

“…Earlier studies reported lipoxygenase and phytoalexins could be important biochemical markers for the development wilt resistant pigeonpea cultivars 12 – 14 . Molecular markers, such as RAPD 15 , simple sequence repeat (SSR) 10 and single nucleotide polymorphism (SNP) 16 ; and quantitative trait loci (QTLs) 11 were reported to be associated with pigeonpea wilt resistance. Singh et al 16 used sequencing-based bulk segregant analysis to map resistance genes for Fusarium wilt in pigeonpea.…”

Section: Introductionmentioning

confidence: 99%

“…Conflicting and inconsistent results suggested complexity in the inheritance of wilt resistance in pigeonpea 18 , 19 . Reports available on molecular markers for pigeonpea wilt resistance was very scanty; also, these reports lacked detailed information on pathogenic races/variants of F. udum 10 . In this scenario, detailed understanding of the molecular factors and signaling pathways behind disease susceptibility or resistance could help in development of resistant plants through different molecular breeding approaches 20 – 22 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection

Purohit

Ghosh

Ganguly

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Vascular wilt caused by Fusarium udum Butler is the most important disease of pigeonpea throughout the world. F. udum isolate MTCC 2204 (M1) inoculated pigeonpea plants of susceptible (ICP 2376) and resistant (ICP 8863) cultivars were taken at invasion stage of pathogenesis process for transcriptomic profiling to understand defense signaling reactions that interplay at early stage of this plant–pathogen encounter. Differential transcriptomic profiles were generated through cDNA-AFLP from M1 inoculated resistant and susceptible pigeonpea root tissues. Twenty five percent of transcript derived fragments (TDFs) were found to be pathogen induced. Among them 73 TDFs were re-amplified and sequenced. Homology search of the TDFs in available databases and thorough study of scientific literature identified several pathways, which could play crucial role in defense responses of the F. udum inoculated resistant plants. Some of the defense responsive pathways identified to be active during this interaction are, jasmonic acid and salicylic acid mediated defense responses, cell wall remodeling, vascular development and pattering, abscisic acid mediated responses, effector triggered immunity, and reactive oxygen species mediated signaling. This study identified important wilt responsive regulatory pathways in pigeonpea which will be helpful for further exploration of these resistant components for pigeonpea improvement.

show abstract

“…Fusarium wilt causes close to US $71 million in annual pigeon pea crop losses (Patil et al, 2017). Disease surveys conducted between 1997 and 2009 in wheat crops grown in Victoria, Australia,estimated average annual yield losses of at least 2-3% or $11-16 million per year due to the crown rot disease (Hollaway & Exell, 2010).…”

Section: Introductionmentioning

confidence: 99%

Fusarium species associated with grain sorghum and mungbean in Queensland

Kelly¹

View full text Add to dashboard Cite

Genetic analysis and molecular resistance to race 2 of Fusarium wilt in pigeonpea [ Cajanus cajan (L.) Millsp.]

Cited by 11 publications

References 27 publications

Breeding, Genetics, and Genomics Approaches for Improving Fusarium Wilt Resistance in Major Grain Legumes

Breeding, Genetics, and Genomics Approaches for Improving Fusarium Wilt Resistance in Major Grain Legumes

Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection

Fusarium species associated with grain sorghum and mungbean in Queensland

Contact Info

Product

Resources

About