Genetic analysis of developmental and adaptive traits in three doubled haploid populations of barley (Hordeum vulgare L.)

Abstract

Study of three interconnected populations identified 13 maturity QTL of which eight collocate with phenology genes, and 18 QTL for traits associated with adaptation to drought-prone environments. QTL for maturity and other adaptive traits affecting barley adaptation were mapped in a drought-prone environment. Three interconnected doubled haploid (DH) populations were developed from inter-crossing three Australian elite genotypes (Commander, Fleet and WI4304). High-density genetic maps were constructed using genotyping by sequencing and single nucleotide polymorphisms (SNP) for major phenology genes controlling photoperiod response and vernalization requirement. Field trials were conducted on the three DH populations in six environments at three sites in southern Australia and over two cropping seasons. Phenotypic evaluations were done for maturity, early vigour, normalized difference vegetation index (NDVI) and leaf chlorophyll content (SPAD), leaf waxiness and leaf rolling. Thirteen maturity QTL were identified, all with significant QTL × environment interaction with one exception. Eighteen QTL were detected for other adaptive traits across the three populations, including three QTL for leaf rolling, six for leaf waxiness, three for early vigour, four for NDVI, and two QTL for SPAD. The three interlinked populations with high-density linkage maps described in this study are a significant resource for examining the genetic basis for barley adaptation in low-to-medium rainfall Mediterranean type environments.

Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense

“…From an agronomic point of view, drought and/or heat tolerance ultimately result in yield stability (von Korff et al , 2006; Worch et al , 2011; Tondelli et al , 2014; Mikołajczak et al , 2016), which can be achieved by three different strategies: escape, avoidance, or tolerance. Escape strategists prevent excessive yield loss by stress-triggered reprogramming of reproductive development, ultimately leading to premature flowering (for a recent study, see Obsa et al , 2016). Avoidance comprises physiological responses that improve water use efficiency, such as stomatal closure (e.g.…”

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

“…An insignificant association of embryo production with plant regeneration, invaluable for the creation of mapping populations for genetic and genomic studies (Forster et al 2007) and the simplification of genome sequence assembly (Dunwell 2010). Through self-pollination, DH populations can be propagated indefinitely and stored as seed, thus allowing for multi-year and multi-location phenotyping for QTL discovery (Guo et al 2013), which has been particularly useful in cereal species (Cabral et al 2014;Sannemann et al 2015;Obsa et al 2016). In breeding programs, DH production replaces inbred line development through repeated self-pollination, so that major reductions in the time to cultivar release are achieved (Dwivedi et al 2015).…”

Quantitative trait loci for yield and grain plumpness relative to maturity in three populations of barley (Hordeum vulgare L.) grown in a low rain-fall environment

“…The primers used for detecting presence/absence of Vrn-H2 and for detecting SNP in HvCO2 and Ppd-H1 by high-resolution melting curve method were provided in supplementary file of [36]. KASP assays of HvAP2 , HvCO1 , HvFT5 , HvGI , HvPhyB , HvPhyC and HvTFL1 , were provided by LGC genomics; the sequences are in supplemental file, S1 File.…”