Genetic analysis of growth cone guidance in drosophila: Fasciclin II functions as a neuronal recognition molecule

Grenningloh, Gabriele; Rehm, E. Jay; Goodman, Corey S.

doi:10.1016/0092-8674(91)90571-f

Cited by 454 publications

(306 citation statements)

References 37 publications

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“…Anti-FasII-The anti-FasII antibody labeled a 97-kDa band in Western blot, which was absent in FasII null mutants (Grenningloh et al, 1991;Mathew et al, 2003). The labeling pattern observed in this study is identical to previous reports (Grenningloh et al, 1991;Landgraf et al, 2003;Mathew et al, 2003).…”

Section: Antibody Characterizationsupporting

confidence: 79%

Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment

MacNamee

Liu

Gerhard

et al. 2016

J of Comparative Neurology

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Anatomical, molecular, and physiological interactions between astrocytes and neuronal synapses regulate information processing in the brain. The fruit fly Drosophila melanogaster has become a valuable experimental system for genetic manipulation of the nervous system and has enormous potential for elucidating mechanisms that mediate neuron glia interactions. Here, we show the first electrophysiological recordings from Drosophila astrocytes and characterize their spatial and physiological relationship with particular synapses. Astrocyte intrinsic properties were found to be strongly analogous to those of vertebrate astrocytes, including a passive current-voltage relationship, low membrane resistance, high capacitance, and dye-coupling to local astrocytes. Responses to optogenetic stimulation of glutamatergic pre-motor neurons were correlated directly with anatomy using serial electron microscopy reconstructions of homologous identified neurons and surrounding astrocytic processes. Robust bidirectional communication was present: neuronal activation triggered astrocytic glutamate transport via Eaat1, and blocking Eaat1 extended glutamatergic interneuron-evoked inhibitory post-synaptic currents in motor neurons. The neuronal synapses were always located within a micron of an astrocytic process, but none were ensheathed by those processes. Thus, fly astrocytes can modulate fast synaptic transmission via neurotransmitter transport within these anatomical parameters. Graphical AbstractCorresponding Author: Sarah E. MacNamee, University of Arizona Dept. of Neuroscience, 1040 E 4 th St GS Rm 611, Tucson AZ 85721, (520) 621 6671, Smac3@email.arizona.edu. Role of AuthorsAll authors had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: SEM, LAO. Acquisition of data: SEM, KEL, SG, CTT, RDF, AC. Analysis and interpretation of data: SEM, KEL, CTT. Drafting of the manuscript: SEM. Critical revision of the manuscript for important intellectual content: LAO, LPT, AC. Statistical analysis: SEM. Obtained funding: LAO, LPT, AC. Administrative, technical, and material support: none. Study supervision: LAO, LPT. Conflict of Interest StatementThe authors have no conflict of interest to disclose. Data AccessibilityData generated in the lab and the subsequent analyses will be made available upon request to the Oland/Tolbert laboratory by qualified individuals as long as doing so would not compromise intellectual property interests or interfere with publication. Any shared data would include notations, standards, and the like that are necessary for interpretation of the data. HHMI Author Manuscript HHMI Author Manuscript HHMI Author ManuscriptThe authors show the first recordings from Drosophila astrocytes and find that their electrophysiological properties are strongly analogous to those of vertebrate astrocytes. Using correlative electron microscopy and physiology, they found no glial ensheathment of glutamatergic synap...

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Section: Antibody Characterizationsupporting

confidence: 79%

Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment

MacNamee

Liu

Gerhard

et al. 2016

J of Comparative Neurology

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“…In wild-type late embryos, as it is the case in y 1 P{SUPor-P}vav KG02022 embryos (Fig. 3A), the anti-FasII antibody labels three bundles of axons running longitudinally along each side of the midline (Grenningloh et al, 1991). While we could not detect any defects in vav mutant embryos at stages 13 and 14 (supplemental Fig.…”

Section: Embryonic Midline Guidance Defects In Vav Mutantscontrasting

confidence: 41%

The Guanine Exchange FactorvavControls Axon Growth and Guidance duringDrosophilaDevelopment

Malartre

Ayaz²,

Amador³

et al. 2010

J. Neurosci.

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The Vav proteins are guanine exchange factors (GEFs) that trigger the activation of the Rho GTPases in general and the Rac family in particular. While the role of the mammalian vav genes has been extensively studied in the hematopoietic system and the immune response, there is little information regarding the role of vav outside of these systems. Here, we report that the single Drosophila vav homolog is ubiquitously expressed during development, although it is enriched along the embryonic ventral midline and in the larval eye discs and brain. We have analyzed the role that vav plays during development by generating Drosophila null mutant alleles. Our results indicate that vav is required during embryogenesis to prevent longitudinal axons from crossing the midline. Later on, during larval development, vav is required within the axons to regulate photoreceptor axon targeting to the optic lobe. Finally, we demonstrate that adult vav mutant escapers, which exhibit coordination problems, display axon growth defects in the ellipsoid body, a brain area associated with locomotion control. In addition, we show that vav interacts with other GEFs known to act downstream of guidance receptors. Thus, we propose that vav acts in coordination with other GEFs to regulate axon growth and guidance during development by linking guidance signals to the cytoskeleton via the modulation of Rac activity.

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“…1B, the larval MBs are topologically similar to the adult MBs but have only two orthogonal lobes, ␣ L and ␤ L (3,5,6). Internally, the peduncles and lobes have simple concentric organization, in which the FAS II (19) and DIF (20) proteins are expressed homogeneously except for the central, unstained core (Fig. 4A).…”

Section: Resultsmentioning

confidence: 99%

Genetic control of development of the mushroom bodies, the associative learning centers in the Drosophila brain, by the eyeless, twin of eyeless , and dachshund genes

Kurusu

Nagao

Walldorf

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

123

125

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Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. By high-resolution neuroanatomy, we show that eyeless (ey), twin of eyeless, and dachshund (dac), which are implicated in eye development, also are expressed in the developing MBs. Mutations of ey completely disrupted the MB neuropils, and a null mutation of dac resulted in marked disruption and aberrant axonal projections. Genetic analyses demonstrated that, whereas ey and dac synergistically control the structural development of the MBs, the two genes are regulated independently in the course of MB development. These data argue for a distinct combinatorial code of regulatory genes for MBs as compared with eye development and suggest conserved roles of Pax6 homologs in the genetic programs of the olfactory learning centers of complex brains.

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Genetic analysis of growth cone guidance in drosophila: Fasciclin II functions as a neuronal recognition molecule

Cited by 454 publications

References 37 publications

Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment

Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment

The Guanine Exchange FactorvavControls Axon Growth and Guidance duringDrosophilaDevelopment

Genetic control of development of the mushroom bodies, the associative learning centers in the Drosophila brain, by the eyeless, twin of eyeless , and dachshund genes

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