Genetic analysis ofCitrus aurantiumL. (Rutaceae) cultivars by ISSR molecular markers

Abstract

Eight cultivars of Citrus aurantium L., showing peculiar morphological traits of the fruits and leaves, were analyzed genetically. Inter simple sequence repeat (ISSR) was chosen as molecular markers because they represent a highly efficient system for investigating variability at intraspecific level. The particular morphological traits were discussed, the genetic identity and distance matrix based on Nei index was calculated, and the unweighted pair group method with arithmetic mean (UPGMA) dendrogram was generated. A total of 53 clearly distinguished DNA bands were considered for the ISSR analysis of which 24 were polymorphic. A basic C. aurantium fingerprinting pattern was obtained. The ISSR profiles allowed to discriminate only two cultivars, while the other six ones did not show polymorphisms, although their phenotypes were notably different, indicating that mutations must have occurred without significantly modifying the DNA length between the simple sequence repeats. The results showed a very low level of genetic variability among the cultivars; ‘‘Canaliculata’’ formed a separated cluster with C. sinensis Osbeck, suggesting a probable hybrid origin derived from crossing between sour and sweet orange. This study confirmed the reliability and reproducibly of ISSR markers for Citrus fingerprinting purposes, although some morphological differences could not be detected; therefore, both morphological and genetic characters must be considered for cultivar or variety identification

Intercultivar Diversity of Sour Orange (Citrus aurantium L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial Analysis

“…However, it was recently demonstrated in citrus that the mutation of SSR markers is a rare event between two clonally propagated cultivars [26]. This conclusion concerning extremely low/absent genetic diversity is in accordance with previous work [20][21][22][23][24][25][26]49]. Studies using multilocus genotyping with dominant markers found higher diversity than studies using SSR markers [22,24].…”

Identification of genetic diversity among mutant lemon and mandarin varieties using different molecular markers

“…In this work, codominant SSR markers (Biswas et al, 2011), dominant ISSR markers (Lombardo et al, 2011), and SSCP markers that is sensitive and informative on mutation detection (DeğirmenciKarataş et al, 2010) were chosen for the determination of seedless mutant mandarins from nonmutant seedy parent mandarin and thornless lemons from nonmutant thorny parent lemon in citrus mutation breeding. In this study the genome was scanned at a high number of loci, as each of the 82 different primer combinations amplified an excess of 363 bands.…”

Chemical Compositions and Antioxidant Activities of Essential Oils, and Their Combinations, Obtained from Flavedo By-Product of Seven Cultivars of Sicilian Citrus aurantium L.

“…C. aurantium ‘Canaliculata’ (Voucher No. 109736) has lemon-like leaves and fruits with aureus-rubescent tuberculate epicarp; thick, spongy, flavorful mesocarp; and juicy, acidic flesh [ 36 ]. C. aurantium ‘Consolei’ (Voucher No.…”