Genetic analysis of
            <i>Toxocara cati</i>
            (Nematoda: Ascarididae) from Guangdong province, subtropical China

He, Xi; Lv, Meng-Na; Li, Guohua; Lin, Rui-Qing

doi:10.1080/24701394.2016.1258404

Cited by 11 publications

(13 citation statements)

References 35 publications

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“…Having used PCR diagnosis for the Ascarididae family nematodes and different primer pairs targeting various genes for discrimination of the species, He et al . (6) reported that cox1 gene sequencing is enough to accurately distinguish and identify T. cati . Similarly, in a study performed in Iran on the ascaridoid nematodes, Mikaeli et al .…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey

Oğuz

Özdal

Deger

2018

Journal of Veterinary Research

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IntroductionToxocara canis and Toxocara cati are roundworms of dogs and cats. The purpose of this study was to investigate the infection caused by these ascarids in cats and dogs, using microscopic and molecular analysis methods.Material and MethodsAdult ascarids were gathered from the faeces of dogs and cats in Van province, in 2015–2016. Existing keys and PCR sequencing of the ITS-2 fragment were used to identify the morphological features of the parasite species.ResultsIt was observed that out of 20 adult ascarids, 17 and 3 were found to be Toxocara canis and Toxocara cati, respectively. The ITS-2 gene region was amplified by PCR to perform molecular analysis. Genotyping indicated that the dogs and cats were infected with T. canis and T. cati, respectively, and none had Toxascaris leonina.ConclusionTo the best of our knowledge, this is the first report on the molecular characteristics of adult ascaridoid nematodes from cats and dogs in Turkey. The molecular approaches established in this study enable molecular identification and genetic structure studies of the ascaridoids.

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Section: Discussionmentioning

confidence: 99%

Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey

Oğuz

Özdal

Deger

2018

Journal of Veterinary Research

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“…Previous studies have commonly used the cox1 gene as genetic marker to identify Spirometra spp., Ascaris suum, Toxocara cati, Ancylostoma ceylanicum, Echinococcus granulosus [4,7,11,14,15]. Some authors have analysed the ribosomal (ITS1 and 18S) and mitochondrial (ND1) gene sequence of T. vitulorum.…”

Section: Discussionmentioning

confidence: 99%

“…The genus Toxocara includes parasitic nematodes of human and animal public health significance, such as T. canis, T. cati, Toxascaris leonina, T. malaysiensis, and T. vitulorum [7]. Of these, Toxocara vitulorum is a nematod living in small intestines of especially at a young age (1-3 months) cattle, water buffalos, and zebu.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, DNA techniques have used widely to the identification and genetic differentiation of T. vitulorum and other Toxocara species [6,7,10,12]. Various studies have demonstrated that some genetic regions, such as the mitochondrial (mt) DNA and nuclear ribosomal DNA (rDNA) could ensure reliable markers for determine the phylogenetic relationships among the Toxocara species [16,19,20,22].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Characterization of Toxocara vitilorum in Turkey by Mitochondrial Gene Markers (cox1)

Oğuz

2018

Acta Scientiae. Vet.

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Background: Toxocara vitulorum is a involved in the Ascaridoidea family and is a large roundworm with a semi translucent, soft body surface and pinkish color. Female worms measure 8-30cm in length, male worms 6-25cm. The major hosts of T.vitulorum are buffalo (Bubalus bubalis) and cattle (Bos species) in the humid tropics of Asia, Africa and South America. The diagnosis of T. vitulorum infections is usually made by observing characteristic eggs in routine fecal examination. Serological methods are also used to diagnose Toxocariasis. However, in recent years, PCR, a new generation molecular diagnostic method, has been used. The genetic structure of T. vitulorum is little known compared with data available from other parasites. The present sutudy was designed to determine the T. vitulorum isolates by the genetic characterization of the mitochondrial cytochrome c oxidase subunit I (cox1) gene.Materials, Methods & Results: Adult worms were collected from the feces of two calves (East Anatolian Red) during visits to the clinic at the Department of Internal Medicine of Van Yuzuncu Yil University, Faculty of Veterinary Medicine. Worms were washed thoroughly in 0.85 % saline to remove any debris and fixed into 70 % ethanol. After repeated and thoroughly washing the specimens, total genomic DNA of parasite extraction was performed be employing DNA extraction reagent kit (Thermo, GeneJET Genomic DNA Purification Kit) according to manufacturer’s recommendations. After DNA amplification, a 446 bp fragment of cox1 of T. vitulorum were obtained in all three isolates. All generated sequences were registered in GenBank database with accession numbers including MG905159, MG911729 and MG911730. The cox1 of T. vitulorum examined differed from another two isolates extracted from Germany beef cattle (KY313642.1) and Sri Lanka buffalo calf (FJ664617.1) at NCBI database. The MEGA 7 software was employed to calculate intra-species distance and similarity. The intra-species distance rate and similarity among the isolates were 0.005 and 99.995%, respectively. The cox 1 sequence of T. vitulorum did not differ from an isolate from Germany, but differed more from isolate from Sri Lanka. The phylogenetic tree that was constructed using the Neighbor-Joining (NJ) method. Bootstrap support (Bp) for ML trees was calculating 1000 bootstrap replicates. This results indicate that both the different species of Toxocara are host-specific and each member of the genus Toxocara spp. has a different about the molecular sequences. We used the phylogenies from the Maximum Parsimony (MP) method to construct another phylogenetic tree based on the cox1 (mtDNA) gene. The results again display that the cattle-calves (East Anatolian Red) isolates from Turkey homology with that obtained from the Germany beef cattle (accession no. KY313642.1).Discussion: The genetic analysis of parasites is a crucial factor in terms of determining epidemiology and the control parasitic diseases of humans and animals. Toxocara vitulorum is the most common gastrointestinal helmints infecting ruminants particularly in tropical regions. Phylogenetic analysis revealed that T. vitulorum is 100% homology with related to sequence of T. vitulorum from Germany. The characterization of cox1 region can provides a foundation for accurate identification of some helminth species using PCR. Even though the small sample size, the obtained results might provide useful information for further phylogenetic studies on the family Ascaridae.

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“…Toxocara cati and Toxascaris leonina are common gastrointestinal parasites of cats [6,7], both species may affect host fitness and even impair host health [8,9]. Infection of Toxascaris is generally with considerably lower prevalence compared to Toxocara infections in domestic cats [10].…”

Section: Introductionmentioning

confidence: 99%

Ascarid infection in wild Amur tigers (Panthera tigris altaica) in China

Peng

Yao

Liu³

et al. 2020

BMC Vet Res

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Background: Wild Amur tigers are a sparsely populated species, and the conservation of this species is of great concern worldwide, but as an important health risk factor, parasite infection in them is not fully understanding. Results: In this study, sixty-two faecal samples were collected to investigate the frequency and infection intensity of Toxocara cati and Toxascaris leonina in wild Amur tigers. The T. cati and T. leonina eggs were preliminary identified by microscopy, and confirmed by molecular techniques. Infection intensity was determined by the modified McMaster technique. Phylogenetic trees demonstrated that T. cati of wild Amur tiger had a closer relationship with which of other wild felines than that of domestic cats. T. leonina of Amur tiger and other felines clustered into one clade, showing a closer relationship than canines. The average frequency of T. cati was 77.42% (48/62), and the frequency in 2016 (100%) were higher than those in 2013 (P = 0.051, < 0.1; 66.6%) and 2014 (P = 0.079, < 0.1; 72.2%). The infection intensity of T. cati ranged from 316.6 n/g to 1084.1 n/g. For T. leonina, only three samples presented eggs when the saturated sodium chloride floating method was performed, indicating that the frequency is 4.83% (3/62). Unfortunately, the egg number in faecal smears is lower than the detective limitation, so the infection intensity of T. leonina is missed. Conclusions: This study demonstrated that ascarids are broadly prevalent, and T. cati is a dominant parasite species in the wild Amur tiger population.

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Genetic analysis of Toxocara cati (Nematoda: Ascarididae) from Guangdong province, subtropical China

Cited by 11 publications

References 35 publications

Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey

Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey

Genetic Characterization of Toxocara vitilorum in Turkey by Mitochondrial Gene Markers (cox1)

Ascarid infection in wild Amur tigers (Panthera tigris altaica) in China

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