Genetic analysis of in vitro wheat somatic embryogenesis

Buyser, J. De; Marcotte, J. L.; Henry, Y.

doi:10.1007/bf00024553

Cited by 20 publications

(6 citation statements)

References 23 publications

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“…Endogenous factors, such as the level of growth regulators within plant explants, together with the interaction between endogenous and exogenous hormones may also influence the embryogenic response [32]. Moreover, the ability to develop somatic embryogenesis was also shown to be under genetic control [33], and particularly in sweet potato, the embryogenic response depended on the genotype [10,14,34]. In this study, the absence of embryogenic response was confirmed for cv.…”

Section: Discussionsupporting

confidence: 53%

Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.)

Triqui

Guedira

Chlyah

et al. 2007

Comptes Rendus. Biologies

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Lateral buds of six cultivars of sweet potato were induced to form embryogenic callus in a culture medium solidified with two types of gelling agents, Agar or Gelrite, and supplemented with various concentrations of auxins, 2,4-D, 2,4,5-T and Picloram. Of the six cultivars screened, only three gave an embryogenic response. Best results with an average of 3.53% embryogenic response were obtained with the medium solidified with Agar, while in Gelrite only 0.45% of lateral buds gave rise to embryogenic callus. The interaction between the genotype and auxins was highly significant; particularly the optimal response was obtained with cv. Zho and 865 yielding 10.7 and 14.7% somatic embryogenesis, respectively, in the medium containing 2,4,5-T or Picloram. The plant conversion was dramatically improved by subculture of the embryogenic callus on the medium with the combination of 1 µM 2,4-D and 1 µM Kinetin or 5 µM ABA alone before transfer of mature embryos onto hormone-free medium. The embryogenic callus of sweet potato and its sustained ability to further regenerate plants have regularly been maintained for several years by frequent subculture in 5 µM 2,4,5-T or the combination of 10 µM 2,4-D and 1 µM BAP or kinetin. The embryo-derived plants seemed apparently genetically stable and similar to the hexaploid parental plants, based on morphological analysis and their ploidy level determined by using flow cytometry. To cite this article: Z.

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Section: Discussionsupporting

confidence: 53%

Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.)

Triqui

Guedira

Chlyah

et al. 2007

Comptes Rendus. Biologies

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“…The hexaploid wheat (Triticum aestivum L., 2n = 6x = 42) CS was used as a reference due to its high performances in somatic tissue culture (De Buyser et al, 1992). CS DT and NT lines were provided by S.M.…”

Section: Methodsmentioning

confidence: 99%

“…In plants either individual somatic or gametic cells can give rise to true embryos through in vitro culture. In CS wheat (Triticum aestivum) about 95% of the immature embryos cultured on a simple medium produce embryogenic cultures (De Buyser et al, 1992). Visual observation and histology confirm that the compact, yellowish embryogenic cultures possessed embryos and green areas at their surface and unorganized parts (i.e.…”

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confidence: 99%

Immunological Detection of Potential Signal-Transduction Proteins Expressed during Wheat Somatic Tissue Culture

Nato¹,

Mirshahi²,

Tichtinsky³

et al. 1997

Plant Physiology

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An immunochemical approach was used to detect the expression of putative guanine nucleotide-binding proteins (C-proteins), arrestin, and nucleoside diphosphate kinases during wheat (Triticum aestivum) tissue culture initiated from immature embryos. Both the soluble and membrane extracts from the immature embryos revealed bands of 58, 40, and 16 kD with antibodies to C-protein ( a subunit), arrestin, and nucleoside diphosphate kinase, respectively. These proteins were overexpressed in vitro in both nonembryogenic callus and embryogenic cultures. An additional soluble protein (32 kD) was detected by anti-Gcu antibodies in cultured tissues but not in immature embryos, suggesting a possible function in cell multiplication. Moreover, somatic embryogenesis was associated with the appearance of a 29-kD protein reactive with anti-arrestin antibodies, both in soluble and membrane fractions. Tissue-cultured genetic stocks of Chinese Spring wheat, including the disomic, 36 ditelosomic, and 6 nullisomic-tetrasomic wheat lines, were used to ascertain the chromosomal location of the genes encoding the 29-kD arrestin-like protein. The lack of a signal with the nonembryogenic ditelosomic 3 D short chromosome arm line suggests that the 3 D long chromosome arm possesses at least one gene involved in the expression of the 29-kD protein. The putative role of the 29-kD protein in signal-transduction regulating embryogenesis is discussed.

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“…Genetic control of regeneration has been reported to vary from dominant effect of nuclear genes in corn (Hodges et al, 1986) to additive gene action in wheat and rice (Lazar et al, 1984;Miah et al, 1985;Quimio and Zapata, 1990). De Buyser et al, (1992), assessed the involvement of few genes controlling regeneration in Brazilian wheat genotypes. The presence of a system exhibiting genetic variation for in vitro regeneration is important for enhancing regeneration responses.…”

Section: Introductionmentioning

confidence: 99%

A Study of in vitro Regeneration in Relation to Doses of Growth Regulators in Hybrids of Upland Cotton

Rauf

Rahman

2005

Plant Cell Tiss Organ Cult

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The frequency of in vitro callus induction and plant regeneration is influenced by several factors, including composition of culture medium, explant source, and the genotype. Crosses between regenerable and nonregenerable upland cotton cultivars were evaluated for hybrid vigour towards regeneration responses, which is consequential in recalcitrant crop species like cotton where regeneration is limited only to a few cultivars. The results indicated that regenerable and non-regenerable parental cultivars had similar potential of producing callus, but differed in producing callus weight and embryogenic calli. Mean performance of crosses, regarding callus induction, callus weight, callus growth rate, percent embryo induction, and percentage of germinating embryos, deviated considerably from the performance of their parents, signifying the presence of hybrid vigour for the expression of these traits. Magnitude of hybrid vigour varied across hormonal levels. Genetic component was evident for all the traits although of lower magnitude. The results indicated that genetic component in the phenotypic expression of callus growth, percentages of embryo induction and germinating embryos was higher than that of callus induction, callus weight and percentage of embryogenic calli. Hormonal concentration in the media had affect on the degree of gene expression responsible for regeneration in upland cotton. Over, partial-and additive-dominance types of gene effects were apparent in the expression of these traits. Genotype · growth regulator level interaction caused considerable variation in the expression of regeneration responses, suggesting that determination of specific level of growth regulator concentration in the medium was necessary for a particular genotype to obtain optimum response. Genotype · explant source interaction was, however, relatively less important. Differences among genotypes for percent embryo induction were clearly evident.

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Genetic analysis of in vitro wheat somatic embryogenesis

Cited by 20 publications

References 23 publications

Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.)

Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.)

Immunological Detection of Potential Signal-Transduction Proteins Expressed during Wheat Somatic Tissue Culture

A Study of in vitro Regeneration in Relation to Doses of Growth Regulators in Hybrids of Upland Cotton

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