Genetic analysis of lactoferrin content in bovine milk

Abstract

Bovine lactoferrin (LF) is mainly present in milk and shows important physiological and biological functions. The aim of this study was to estimate the heritability and correlation values of LF content in bovine milk with different economic traits as milk yield (MY), fat and protein percentages, and somatic cell score (SCS). Variance components of the studied traits were estimated by REML using a multiple-trait mixed model. The obtained heritability (0.22) for LF content predicted using mid-infrared spectrometry (pLF) suggested the possibility of animal selection based on the increase of LF content in milk. The phenotypic and genetic correlation values calculated between pLF and SCS were moderate (0.31 and 0.24, respectively). Furthermore, a preliminary study of bovine LF gene polymorphism effects was performed on the same production traits. By PCR, all exons of the LF gene were amplified and then sequenced. Three new polymorphisms were detected in exon 2, exon 11, and intron 8. We examined the effects of LF gene polymorphisms of exons 2, 4, 9, 11, and 15, and intron 8 on pLF, MY, fat and protein percentages, and SCS. The different observed effects did not reach a significant level probably because of the characteristics of the studied population. However, the results were promising, and LF may be a potential indicator of mastitis. Further studies are necessary to evaluate the effect of genetic selection based on LF content on the improvement of mastitis resistance.

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

“…This observation confirms the previous conclusions of many reports showing the increase in lactoferrin content in milk from infected udders compared with healthy cows (Kawai et al 1999, Hagiwara et al 2003, Cheng et al 2008b, Arnould et al 2009). LTF level in milk is believed to be a good indicator of mastitis because of a high positive correlation between LTF and SCC (Lindmark-Mansson 2006, Soyeurt et al 2007, Arnould et al 2009) and is also useful in detecting the false-positive results of screening tests for antibiotic residues in milk (Malinowski et al 2008). Cheng et al (2008b) observed a significant positive correlation of lactoferrin concentrations between serum and milk in early phase of the immune response.…”

“…The low LTF concentration during the first 2 months of lactation and the highest during the last 3 months could be explained by the dilution effect observed by Arnould et al (2009) and Soyeurt et al (2007) who obtained a negative genetic correlation between daily milk production and LTF content of -0.25 and -0.36, respectively. A significant increase in LTF concentration in the following stages of lactation was in agreement with the results obtained by other authors (Hagiwara et al 2003, Cheng et al 2008a.…”

Hubungan Polimorfisme Gen Laktoferin dengan Kualitas Susu pada Sapi Perah Friesian-Holstein

“…Beberapa laporan menyebutkan aktivitas antibakterial laktoferin baik pada penelitian in vitro maupun in vivo (Arnould et al, 2009;Nibbering et al, 2001;Pawlik et al, 2009, Wakabayashi et al, 2006. Laktoferin menyebabkan membran bakteri gram negatif tidak stabil karena terjadi pemblokiran interaksi antara LPS dengan LPS signaling (CD 14) (Rainard and Riollet, 2006).…”

“…Isolasi DNA dari sampel darah dilakukan dengan menggunakan metode ekstraksi phenol-chloroform (Sambrook et al 1989) yang telah dimodifikasi untuk sampel darah yang disimpan dalam alkohol. Isolasi DNA dari sampel susu dilakukan dengan menggunakan metode ekstraksi DNA dari sel somatik (Arnould et al, 2009 …”

“…Arnould et al (2009) yang melakukan penelitian mengenai kontrol gen laktoferin pada populasi sapi perah Belgian Blue, NonRed Holstein, Holstein, Jersey, Brown Swiss dan Montbeliarde terhadap produksi susu, kadar protein dan kadar lemak melaporkan adanya hubungan yang positif antara polimorfisme pada exon 2, 4, 9, 11, 15 dan intron 8 dengan produksi susu, kadar protein dan kadar lemak. Ng- Kwai-Hang et al (1990) menerangkan bahwa perbedaan pengaruh varian genotipe terhadap sejumlah sifat kualitas susu dapat terjadi dikarenakan perbedaan ukuran populasi, bangsa sapi, frekuensi munculnya varian genotipe yang dipertimbangkan, parameter yang diperiksa (seperti uji per hari, per laktasi, sifat kualitas susu), dan tentunya yang juga penting adalah metode statistik yang dipakai dalam mengelimasi berbagai faktor seperti pengaruh umur induk, paritas, musim, tahap laktasi, status kesehatan dan variasi genetik itu sendiri.…”