Genetic analysis of resistance to bacterial leaf spot in the heirloom lettuce cultivar Reine des Glaces

Genomics and Marker-Assisted Improvement of Vegetable Crops

“…Many genes of interest have been mapped in lettuce using a diverse set of populations, including F 2 and early BC generations, RILs, BILs, NILs, and diversity panels. The majority of RIL populations used to identify QTLs have been based on intraspecific crosses between two different types of the cultivated lettuce, for example, iceberg  Batavia (Hayes et al, 2014a;Simko et al, 2015a), iceberg  butterhead (Hayashi et al, 2008), iceberg  romaine (Simko et al, 2009;Simko et al, 2011), Latin  Batavia (Mamo et al, 2019;Sandoya et al, 2019), and Batavia  leaf (Simko et al, 2013). Less frequently, however, mapping populations have also been developed from crosses between cultivars of the same type, for example, Batavia  Batavia (Sandoya et al, 2019) and iceberg  iceberg (Aruga et al, 2012;Jenni et al, 2013;Macias-Gonz alez et al, 2019), or between two distinct genotypes of the wild species L. serriola (Bell et al, 2015).…”

“…The majority of RIL populations used to identify QTLs have been based on intraspecific crosses between two different types of the cultivated lettuce, for example, iceberg  Batavia (Hayes et al, 2014a;Simko et al, 2015a), iceberg  butterhead (Hayashi et al, 2008), iceberg  romaine (Simko et al, 2009;Simko et al, 2011), Latin  Batavia (Mamo et al, 2019;Sandoya et al, 2019), and Batavia  leaf (Simko et al, 2013). Less frequently, however, mapping populations have also been developed from crosses between cultivars of the same type, for example, Batavia  Batavia (Sandoya et al, 2019) and iceberg  iceberg (Aruga et al, 2012;Jenni et al, 2013;Macias-Gonz alez et al, 2019), or between two distinct genotypes of the wild species L. serriola (Bell et al, 2015). Various interspecific mapping populations have also been developed, including crosses between iceberg type and L. serriola (probably the most frequently used population in mapping studies) (Argyris et al, 2005;Simko et al, 2009;Truco et al, 2013), butterhead and L. serriola (Uwimana et al, 2012), and leaf type and L. saligna (Jeuken and Lindhout, 2002;Jeuken et al, 2008).…”

“…lactucae formerly known as X. campestris pv. vitians) including both race-specific resistance genes "Xar1" (Hayes et al, 2014b) and "Xcvr" (Wang et al, 2016a) and QTLs for quantitative resistance (Lu et al, 2014;Sandoya et al, 2019). Loci for resistance to viral diseases have been identified for lettuce mosaic virus (LMV)-"mo1" (Nicaise et al, 2003) and Mo-2 (McHale et al, 2009), lettuce dieback caused by tomato bushy stunt virus (TBSV), and lettuce necrotic stunt virus (LNSV)-"Tvr1" (Grube et al, 2005;Simko et al, 2009), turnip mosaic virus (TuMV)-"Tu" (Montesclaros et al, 1997), and big vein caused by mirafiori lettuce big-vein virus (MLBVV)-several QTLs (Hayes et al, 2010;Michelmore, 2010).…”

Bacterial Leaf Spot of Lettuce Disease: An Unpredictable Enemy to the Florida Lettuce Industry

“…Bacterial leaf spot of lettuce (BLS) was discovered in the United States over 100 years ago in South Carolina (Brown 1918). As lettuce acreage increased in the country, the disease became more prominent in major production areas in California and later in Florida because it is favored by the warm, humid conditions during the lettuce season in south Florida, which lasts from October (planting) until mid-April (last harvesting) (Sandoya et al 2019). BLS is an unpredictable threat to the lettuce cultivated on soils with rich organic matter called muck soils in the EAA located on the southern edge of Lake Okeechobee.…”

Innovative breeding technologies in lettuce for improved post-harvest quality

“…“Eruption” x “Reine des Glaces” RIL population Mamo et al, 2019 Bacterial leaf spot Significant marker-trait associations for bacterial leaf spot resistance Diversity panel of cultivars representing the major leaf types Lu et al, 2014 QTL for bacterial leaf spot resistance L. sativa cvs. “Eruption” x “Reine des Glaces” and “Delsay” x “Reine des Glaces” RIL populations Sandoya et al, 2019 Root rot QTL for race 2 major resistance locus L. sativa cvs. “VP1013” x “Patriot” F 2 population Aruga et al, 2012 Lettuce aphid Identified significant marker-trait associations for aphid resistance Diverse panel of wild and cultivated lettuce accessions, representing all major leaf types and of worldwide origin Walley et al, 2017 Post-harvest Shelf life Identification of multiple QTL for shelf life and association physiological, biochemical and biophysical traits, trait data collected in two field trials in two geographic locations L. sativa cv.…”