Genetic analysis of resistance to blackspot (Diplocarpon rosae) in tetraploid roses

Malek, B. von; Debener, Thomas

doi:10.1007/s001220050731

Cited by 78 publications

(63 citation statements)

References 10 publications

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“…Three major genes for resistance against Diplocarpon rosae Wolf have been described for roses. Rdr1 and Rdr2 (Von Malek and Debener 1998;Hattendorf et al 2004) are probably located in the same cluster on LG1 of the ICM, whereas Rdr3 (Whitaker et al 2010) possibly represents a diVerent locus but is not yet intro-duced into a genetic map. A diVerent approach beyond mapping single loci by resistance screens in mapping populations is the ampliWcation of resistance gene-related sequences such as RGAs or NBS markers.…”

Section: Major Genes and Qtlsmentioning

confidence: 99%

Towards a unified genetic map for diploid roses

Spiller

Linde

Oyant³

et al. 2010

Theor Appl Genet

100

150

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We have constructed the Wrst integrated consensus map (ICM) for rose, based on the information of four diploid populations and more than 1,000 initial markers. The single population maps are linked via 59 bridge markers, on average 8.4 per linkage group (LG). The integrated map comprises 597 markers, 206 of which are sequence-based, distributed over a length of 530 cM on seven LGs. By using a larger eVective population size and therefore higher marker density, the marker order in the ICM is more reliable than in the single population maps. This is supported by a more even marker distribution and a decrease in gap sizes in the consensus map as compared to the single population maps. This uniWed map establishes a standard nomenclature for rose LGs, and presents the location of important ornamental traits, such as self-incompatibility, black spot resistance (Rdr1), scent production and recurrent blooming. In total, the consensus map includes locations for 10 phenotypic single loci, QTLs for 7 diVerent traits and 51 ESTs or gene-based molecular markers. This consensus map combines for the Wrst time the information for traits with high relevance for rose variety development. It will serve as a tool for selective breeding and marker assisted selection. It will beneWt future eVorts of the rose community to sequence the whole rose genome and will be useful for synteny studies in the Rosaceae family and especially in the section Rosoideae.

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Section: Major Genes and Qtlsmentioning

confidence: 99%

Towards a unified genetic map for diploid roses

Spiller

Linde

Oyant³

et al. 2010

Theor Appl Genet

100

150

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“…Polyploidization of diploids before crossing with tetraploids allows for tetraploid progeny (Ma et al, 2000;von Malek & Debener, 1998). The use of colchicine on shoot meristems has been the primary method of somatic polyploidization in roses (Basye, 1990;Ma et al, 1997;Roberts et al, 1990;Seminiuk & Arisumi, 1968).…”

Section: Introductionmentioning

confidence: 99%

Trifluralin-mediated polyploidization of Rosa chinensis minima (Sims) Voss seedlings

2005

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Many diploid rose species and cultivars possess valuable traits that can be introgressed into modern tetraploid cultivars. Interspecific, interploidy crosses are possible, but triploid hybrids typically have limited fertility, hindering further breeding and selection. Tetraploidizing diploids before mating with tetraploids can alleviate fertility barriers. The efficiency of trifluralin was investigated for polyploidization of Rosa chinensis minima (2n = 2x = 14) seedlings. Treatments were trifluralin at 0.086% and 0.0086%, colchicine (0.5%), and distilled water and contained 2% dimethyl sulfoxide and a surfactant. Approximately 5 µl of the treatment solution was applied to the apical meristem of seedlings (N = 337, 82-85 per treatment) in the process of cotyledon expansion. Guard cell length, pollen diameter, and root tip squashes of rooted cuttings were used to detect polyploidy in meristematic layer (L)I, LII, and LIII, respectively. Trifluralin (0.086%) was the most effective treatment for polyploidization (LI 20.2%, LII 12.9%, LIII 12.9%), followed by trifluralin (0.0086%) (LI 10.6%, LII 7.1%, LIII 4.7%) and colchicine (LI 2.4%, LII 0%, LIII 0%). Polyploidization consistently occurred from LI inward. Polyploids as a group had reduced pollen stainability and a lower leaflet length to width ratio than diploids. In addition, two diploid seedlings were identified which produce 2n pollen. Considerations in selecting germplasm and generating somatically-induced polyploids from seedlings versus clones for use in breeding are discussed.

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“…As a direct consequence, the inheritance of only a few morphological and physiological traits is known in roses. Thus, male sterility in Rosa setigera Michx., single seasonal blooming, moss character, dwarfness, resistance to blackspot disease, and double corolla are inherited as single dominant genes (Lewis and Basye 1961;Semeniuk 1971;Dubois 1978, 1984;Dubois and De Vries 1987;Von Malek and Debener 1998;Debener 1999). Moreover, the pink colour of the flower (versus white) and the presence of thorns are inherited either as single dominant genes or pairs of complementary genes (Debener 1999).…”

Section: Introductionmentioning

confidence: 99%

Mapping of qualitative and quantitative phenotypic traits in Rosa using AFLP markers

Crespel¹,

Chirollet²,

Durel

et al. 2002

Theor Appl Genet

116

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A segregating population of 91 hybrids issued from a cross between a dihaploid rose, derived from the haploidisation of a modern cultivar, and a diploid species was used to construct linkage maps of the parental genomes. As in other recent genetic studies in Rosa, AFLPs were used as molecular markers. Two segregating qualitative traits, recurrent blooming and double corolla, already known to be inherited as single recessive and dominant genes, respectively, were recorded in the mapping population. A quantitative trait, thorn density of the shoots, was also evaluated in this population. Sixty eight and 108 AFLP markers located on 8 and 6 linkage groups could be analysed in the female and male parent, respectively. The two recorded qualitative phenotypic markers were mapped as well as the quantitative one, after having performed QTL analyses on the parental maps in the latter case. It appears that thorn quantity is controlled by a major and a minor QTL which are located on the same linkage group at 36.5 and 3.2 cM from the single seasonal-blooming gene, respectively.

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Genetic analysis of resistance to blackspot (Diplocarpon rosae) in tetraploid roses

Cited by 78 publications

References 10 publications

Towards a unified genetic map for diploid roses

Towards a unified genetic map for diploid roses

Trifluralin-mediated polyploidization of Rosa chinensis minima (Sims) Voss seedlings

Mapping of qualitative and quantitative phenotypic traits in Rosa using AFLP markers

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