Genetic analysis of variation in micropropagated plants of Melia azedarach L.

Olmos, Sofı́a; Lavia, Graciela Inés; Renzo, M. Di; Mroginski, L. A.; Echenique, Viviana

doi:10.1079/ivp2002319

Cited by 14 publications

(7 citation statements)

References 38 publications

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“…It has been suggested that DNA methylation may contribute to the generation of RAPD polymorphisms (Olmos et al 2002). Although the m 5 C content in genomic DNA of cedar TC progenies was genotype-dependent, it was not possible to establish a correlation between a particular hypomethylation (or hypermethylation) event and the detected RAPD variability.…”

Section: Discussionmentioning

confidence: 99%

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Assessments of somaclonal variation in micropropagated shoots of Cedrus: consequences of axillary bud breaking

Renau‐Morata

Nebauer²,

Arrillaga

et al. 2005

Tree Genetics & Genomes

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Reversed-phase HPLC analysis and random amplified polymorphic DNA (RAPD) markers were used to monitor DNA methylation status and genetic stability of C. atlantica and C. libani shoots generated through axillary bud proliferation. Average DNA methylation in C. atlantica or C. libani seedlings and mature 200-year-old trees of C. libani was 19.8, 19.5 and 22.3%, respectively. These global amounts showed no significant variation after the in vitro establishment of seedling-originated shoot stocks. In contrast, in vitro culture caused a significant decrease in the amount of 5-methylcytosine in genomic DNA of the tissue culture (TC) progenies of one of the adult C. libani genotypes. This DNA demethylation event accompanied an enhancement of the regrowth capacity of this genotype. Detected RAPD variation between mother plants and their TC progenies was species-related, with C. libani TC progenies being genetically more stable than those of C. atlantica. Nevertheless, similarity indices ranged from 0.97 to 1 among mother plants and their TC progenies. Furthermore, the analyses of molecular variance (AMOVA) suggest that RAPD variation among the mother plants and their TC progenies might be considered as not significant. The application of various statistical approaches, including clusterbased genetic distance methods and AMOVA, demonstrates that RAPD markers discriminate C. atlantica and C. libani appropriately.

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Section: Discussionmentioning

confidence: 99%

“…The complexity of somaclonal variation requires the use of several approaches so that plants can be correctly evaluated. This attains a special interest in species with extended growing periods, such as forest trees, where identifying variants as early as possible is essential to avoid the propagation of mutant plants (Olmos et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Assessments of somaclonal variation in micropropagated shoots of Cedrus: consequences of axillary bud breaking

Renau‐Morata

Nebauer²,

Arrillaga

et al. 2005

Tree Genetics & Genomes

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“…Analysis of variation at the nuclear genome level using RAPDs has advantages over RFLPs, as a single primer produces several loci, covering a large portion of the genome [10]. However, uncertain homology of co-migrating fragments in gel electrophoresis and reproducibility have some limitation [11], that can be minimized by carefully adjusting the reaction and detection conditions [12]. Venkatachalam et al [13], by ISSR and RAPD markers has shown that use of high level of cytokinins does not involve any genetic fidelity in all the banana plantlets analyzed.…”

Section: Introductionmentioning

confidence: 99%

RAPD Fingerprint to Appraise the Genetic Fidelity of In Vitro Propagated Araucaria excelsa R. Br. var. glauca Plantlets

et al. 2011

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Randomly amplified polymorphic DNA (RAPD) was used as a tool to assess the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var. glauca with explants taken from orthotropic stem along with their related mother plants after treatment with kinetin, 2iP, BA (0.02-0.26 mg/l) and TDZ (0.001-1 mg/l) to produce axillary shoots. TDZ and kinetin induced more shoot and higher length per explant. Results showed a total of 1,676 fragments were generated with 12 RAPD primers in micropropagated plants and their donor mother plants. The number of loci ranged from 6 in OPB 12-18 in OPY 07 with a size ranging from 250 bp in OPH 19-3500 bp in OPH 11. Cluster analysis of RAPD data using UPGMA (unweighted pair group method with arithmetic average) revealed more than 92% genetic similarities between tissue cultured plants and their corresponding mother plant measured by the Jaccard's similarity coefficient. Similarity matrix and PCoA (two dimensional principal coordinate analysis) resulted in the same affinity. Primers had shown 36% polymorphism. However, careful monitoring of tissue culture derived plants might be needed to determine that rooted shoots are adventitious in origin.

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“…For instance, assessment of 4-and 6-year-old almond plantlets, produced by axillary branching [37] and in vitro regenerated dessert banana from leaf base explants [38] via using RAPD and ISSR analysis, has shown no somaclonal variation or very low rate of polymorphism between regenerants of long-term propagated 8-year-old London plane tree [39]. On the contrary, morphologically similar in vitro-grown plantlets which had no variation in chromosome number and DNA content per cell were shown to contain genetic variability at the DNA sequence level such as Populus deltoides clone L34 (RAPD, [40]), Populus tremuloides (microsatellites, SSR, [41]), Coffee arabica (mtRFLP, RAPD, ISSR, [42]), Eucalyptus tereticornis (rDNA, RFLP, [43]), Robinia ambigua (RAPD, ISSR, [44,45]), Melia azedarach (RAPD, [46]), and many others [47].…”

Section: Discussionmentioning

confidence: 99%

Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers

Akdemir

Süzerer

Tilkat

et al. 2016

Appl Biochem Biotechnol

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Determination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0.226), ISSR (0.220), and AFLP (0.241) showed that micropropagation of pistachio for different periods of time resulted in "reasonable polymorphism" among donor plant and its 18 clones. Mantel's test showed a consistence polymorphism level between marker systems based on similarity matrices. In conclusion, this is the first study on occurrence of genetic variability in long-term micropropagated mature pistachio plantlets. The obtained results clearly indicated that different marker approaches used in this study are reliable for assessing tissue culture-induced variations in long-term cultured pistachio plantlets.

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Genetic analysis of variation in micropropagated plants of Melia azedarach L.

Cited by 14 publications

References 38 publications

Assessments of somaclonal variation in micropropagated shoots of Cedrus: consequences of axillary bud breaking

Assessments of somaclonal variation in micropropagated shoots of Cedrus: consequences of axillary bud breaking

RAPD Fingerprint to Appraise the Genetic Fidelity of In Vitro Propagated Araucaria excelsa R. Br. var. glauca Plantlets

Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers

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