Genetic and antibiotic resistance profiles of thermophilic Campylobacter spp. isolated from quails (Coturnix coturnix japonica) in a Portuguese slaughterhouse

Fraqueza, Maria João; Ribeiro, Simone Alves Mendes; Pereira, Simone Cardoso Lisboa; Fernandes, Maria José; Fernandes, Mariana Lima; Barreto, António Salvador

doi:10.1016/j.foodcont.2015.06.008

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Furthermore, in the 15 strains tested, the occurrence of multidrug resistance (resistant to at least one agent in three or more antimicrobial categories) was high, such that all strains were multidrug-resistant except one ( C. jejuni 65E). A high frequency (69%) of multidrug resistance was also reported by Fraqueza et al (2016) in isolates obtained from quails (in a total of 91 isolates, C. coli n = 87; and C. jejuni n = 4).…”

Section: Resultsmentioning

confidence: 56%

“…On the contrary, all strains were susceptible to chloramphenicol and gentamicin. The observed levels of resistance towards AMP and NA were expected (100%), due to the known widespread resistance to these antibiotics in Portugal ( Duarte et al 2013 , Fraqueza et al, 2016 ). The high susceptibility to chloramphenicol ( CHL ) and gentamicin ( GEN ) was also expected since resistance to these antibiotics is reported to be very rare ( Whitehouse et al, 2018 ).…”

Section: Resultsmentioning

confidence: 98%

“…Campylobacter enumeration and isolation were performed according to ISO 10272-1 and 10272-2 (2017) . Fecal samples were obtained from cecum extracted from broiler carcasses as Fraqueza et al (2016) described. Fecal samples were streaked with a loop on mCCD agar ( Campylobacter selective agar, Neogen) supplemented with Cefoperazone and Amphotericin (Neogen).…”

Section: Methodsmentioning

confidence: 99%

“…Antibiotic susceptibility assessment was performed for ten antibiotics by the standard disc diffusion method, according to EUCAST (2020) and Fraqueza et al (2016) . Isolates were recovered, and bacteria cells were removed from COS agar plates (bioMérieux) to prepare suspensions adjusted to 0.5 MacFarland.…”

Section: Methodsmentioning

confidence: 99%

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Assessment of biofilm formation by Campylobacter spp. isolates mimicking poultry slaughterhouse conditions

et al. 2022

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This research aimed to assess the biofilm formation ability of Campylobacter strains under temperature and oxygen stress conditions, similar to those found in the industrial environment, to explain the persistence of this pathogen on the poultry slaughter line. A collection of C. jejuni and C. coli isolates (n = 143) obtained from poultry samples (cecal content and neck skin), collected at slaughterhouse level, from diverse flocks, on different working days, was genotyped by flaA -restriction fragment length polymorphism ( RFLP ) typing method. A clustering analysis resulted in the assignment of 10 main clusters, from which 15 strains with different flaA -RFLP genotypes were selected for the assessment of biofilm formation ability and antimicrobial susceptibility. Biofilm assays, performed by crystal violet staining method, were conducted with the goal of mimicking some conditions present at the slaughterhouse environment, based on temperature, atmosphere, and contamination levels. Results indicated that many C. jejuni strains with similar flaA -RFLP profiles were present at the slaughterhouse on different processing days. All the strains tested (n = 15) were multidrug-resistant except for one. Biofilm formation ability was strain-dependent, and it appeared to have been affected by inoculum concentration, temperature, and tolerance to oxygen levels. At 10°C, adherence levels were significantly lower than at 42°C. Under microaerobic and aerobic atmospheres, at 42°C, 3 strains ( C. jejuni 46E, C. jejuni 61C, and C. coli 65B) stood out, exhibiting significant levels of biofilm formation. C. jejuni strains 46E and 61C were inserted in clusters with evidence of persistence at the slaughterhouse for a long period of time. This study demonstrated that Campylobacter strains from broilers are capable of forming biofilms under conditions resembling the slaughterhouse environment. These results should be seen as a cue to improve the programs of hygiene implemented, particularly in those zones that can promote biofilm formation.

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Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Assessment of biofilm formation by Campylobacter spp. isolates mimicking poultry slaughterhouse conditions

et al. 2022

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“…from cattle in Poland (20.9%) [52], C. jejuni from broiler carcasses in Belgium (47%) [67] and C. jejuni from chicken meat in France (53.6%) [51] to extremely high in isolates of C. coli from pigs in France (93%) [66], as well as Campylobacter spp. from quails in Portugal (96.7%) [68]. In general, C. coli exhibited higher resistance levels [5].…”

Section: Antimicrobial Resistance In Campylobactermentioning

confidence: 99%

Antimicrobial Resistance of Common Zoonotic Bacteria in the Food Chain: An Emerging Threat

Rozman¹,

Matijašić²,

Možina³

2019

Antimicrobial Resistance - A Global Threat

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Antimicrobial resistance in the food chain is currently a subject of a major interest. The excessive use or rather misuse of antimicrobials coupled with a poor hygiene in the food production chain has led to a rise of resistant zoonotic bacteria, commonly transmitted by food. They pose a serious threat to human health. Campylobacteriosis is the leading bacterial food-borne illness and most commonly reported zoonosis in humans in the European Union for more than a decade. Salmonellosis is most frequently diagnosed in food-borne outbreaks. Fluoroquinolones are considered as critically important for treatment of severe cases of both zoonoses in humans. Due to an extremely prevalent resistant isolates, especially from broilers and meat, also the treatment of human Campylobacter infections with fluoroquinolones has become compromised. Salmonella isolates from poultry and poultry meat tend to be highly resistant to fluoroquinolones as well. Beside the resistance to this group of antibiotics, the threat of multiple drug resistant (MDR) Campylobacter and Salmonella strains is discussed in the light of most recent reports of animal, food and human clinical surveillance systems.

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Antimicrobial-resistant foodborne pathogens in the Middle East: a systematic review

Alsayeqh

Baz²,

Darwish

2021

Environ Sci Pollut Res

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Genetic and antibiotic resistance profiles of thermophilic Campylobacter spp. isolated from quails (Coturnix coturnix japonica) in a Portuguese slaughterhouse

Cited by 7 publications

References 25 publications

Assessment of biofilm formation by Campylobacter spp. isolates mimicking poultry slaughterhouse conditions

Assessment of biofilm formation by Campylobacter spp. isolates mimicking poultry slaughterhouse conditions

Antimicrobial Resistance of Common Zoonotic Bacteria in the Food Chain: An Emerging Threat

Antimicrobial-resistant foodborne pathogens in the Middle East: a systematic review

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