Genetic and biological characterization of a densovirus isolate that affects dengue virus infection

Mosimann, Ana Luiza Pamplona; Bordignon, Juliano; Mazzarotto, Giovanny Camacho Antevêre; Motta, María Cristina M.; Hoffmann, Federico G.; Santos, Claudia Nunes Duarte dos

doi:10.1590/s0074-02762011000300006

Cited by 18 publications

(18 citation statements)

References 39 publications

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“…Reference densovirus strains were selected after blasting the NCBI NT database with a 398 bp fragment assembled with the small RNAs. Numbers in parentheses indicates the reference number of the particular virus stain [14,15,16,17,18,19,20,21,22]. The virus strain identified in this work has been assigned the name Culex tritaeniorhynchus densovirus YN2009 (indicated by a solid arrow).…”

Section: Discussionmentioning

confidence: 99%

“…To understand the evolutionary status of the densovirus identified here, a phylogenetic tree was generated with Mega 4.0 using maximum parsimony and bootstrap 500 methods (Figure 4). The reference densovirus strains [14,15,16,17,18,19,20,21,22] were downloaded from GenBank after blasting the NT database with a 398 bp segment assembled from the small RNAs. The phylogenetic tree obtained infers that newly identified Culex tritaeniorhynchus densovirus YN2009 is a close relative of the mosquito densoviruses prevalent in South and Southwest China.…”

Section: Phylogenetic Analysis Of the Newly Identified Densovirusmentioning

confidence: 99%

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Discovery of DNA Viruses in Wild-Caught Mosquitoes Using Small RNA High throughput Sequencing

Huang

ZhengDa

et al. 2011

PLoS ONE

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BackgroundMosquito-borne infectious diseases pose a severe threat to public health in many areas of the world. Current methods for pathogen detection and surveillance are usually dependent on prior knowledge of the etiologic agents involved. Hence, efficient approaches are required for screening wild mosquito populations to detect known and unknown pathogens.Methodology/principal findingsIn this study, we explored the use of Next Generation Sequencing to identify viral agents in wild-caught mosquitoes. We extracted total RNA from different mosquito species from South China. Small 18–30 bp length RNA molecules were purified, reverse-transcribed into cDNA and sequenced using Illumina GAIIx instrumentation. Bioinformatic analyses to identify putative viral agents were conducted and the results confirmed by PCR. We identified a non-enveloped single-stranded DNA densovirus in the wild-caught Culex pipiens molestus mosquitoes. The majority of the viral transcripts (.>80% of the region) were covered by the small viral RNAs, with a few peaks of very high coverage obtained. The +/− strand sequence ratio of the small RNAs was approximately 7∶1, indicating that the molecules were mainly derived from the viral RNA transcripts. The small viral RNAs overlapped, enabling contig assembly of the viral genome sequence. We identified some small RNAs in the reverse repeat regions of the viral 5′- and 3′ -untranslated regions where no transcripts were expected.Conclusions/significanceOur results demonstrate for the first time that high throughput sequencing of small RNA is feasible for identifying viral agents in wild-caught mosquitoes. Our results show that it is possible to detect DNA viruses by sequencing the small RNAs obtained from insects, although the underlying mechanism of small viral RNA biogenesis is unclear. Our data and those of other researchers show that high throughput small RNA sequencing can be used for pathogen surveillance in wild mosquito vectors.

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Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analysis Of the Newly Identified Densovirusmentioning

confidence: 99%

Discovery of DNA Viruses in Wild-Caught Mosquitoes Using Small RNA High throughput Sequencing

Huang

ZhengDa

et al. 2011

PLoS ONE

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“…aegypti egg densities [129]. A direct inhibitory effect of MDVs on arboviral replication in cell lines has also been shown [130,131,132] which could work synergistically with pathogenic effects on the mosquito vector. However, co-infection of MDV and CHIKV in adult Ae.…”

Section: Releasing Mosquitoes For Disease Controlmentioning

confidence: 99%

Biological Control of Mosquito Vectors: Past, Present, and Future

Benelli

Jeffries

Walker

2016

Insects

337

216

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Mosquitoes represent the major arthropod vectors of human disease worldwide transmitting malaria, lymphatic filariasis, and arboviruses such as dengue virus and Zika virus. Unfortunately, no treatment (in the form of vaccines or drugs) is available for most of these diseases and vector control is still the main form of prevention. The limitations of traditional insecticide-based strategies, particularly the development of insecticide resistance, have resulted in significant efforts to develop alternative eco-friendly methods. Biocontrol strategies aim to be sustainable and target a range of different mosquito species to reduce the current reliance on insecticide-based mosquito control. In this review, we outline non-insecticide based strategies that have been implemented or are currently being tested. We also highlight the use of mosquito behavioural knowledge that can be exploited for control strategies.

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“…An increasing number of arthropod-specific viruses (ASVs) are being discovered in haematophagous arthropods over the world [8], and there is a growing interest on mesoniviruses interactions with viruses of public health concern to develop strategies that could curb arbovirus transmission through dual infection and competition, as implemented with the Wolbachia mediated strategy [9]. Some studies suggested for instance that ISVs negatively affect the fitness of West Nile (WNV) [10][11][12] and dengue (DENV) [13,14] viruses in the mosquito, while other studies demonstrated that there is no effect [15] or even an enhancing effect [11]. However, there are still few studies on mesoniviruses phenotypic behaviour in mosquito vectors.…”

Section: Introductionmentioning

confidence: 99%

Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal

Gaye

Diagne

Ndiaye

et al. 2020

Emerging Microbes & Infections

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The mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named Dianke virus (DKV) from common mosquitoes from eastern Senegal. Actually, their vector competence for this newly described DKV is unknown. We, therefore, estimated the vector competence of Culex tritaeniorhynchus, Culex quinquefasciatus, Aedes aegypti, and Anopheles gambiae mosquitoes collected in Senegal for DKV using oral infection. Whole bodies, legs/wings, and saliva samples were tested for DKV by RT-PCR to estimate infection, dissemination, and transmission rates. The infectivity of virus particles in the saliva was confirmed by infecting C6/36 cells. Virus transmission rates were up to 95.45% in Culex tritaeniorhynchus, 28% in Cx. quinquefasciatus and 9.09% in Aedes aegypti. Viral particles in the saliva were confirmed infectious by C6/36 cell culture. An. gambiae was able to disseminate DKV only at 20 days post-infection. This study shows that Culex mosquitoes are more competent than Ae. aegypti for DKV, while Anopheles gambiae is not likely a competent vector.

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Genetic and biological characterization of a densovirus isolate that affects dengue virus infection

Cited by 18 publications

References 39 publications

Discovery of DNA Viruses in Wild-Caught Mosquitoes Using Small RNA High throughput Sequencing

Discovery of DNA Viruses in Wild-Caught Mosquitoes Using Small RNA High throughput Sequencing

Biological Control of Mosquito Vectors: Past, Present, and Future

Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal

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