Genetic and chemical comparison of Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii)

Abstract

Boi and its original plant Sinomenium acutum from Japan were compared with Seifuto and its botanical origins from China in terms of their internal transcribed spacer (ITS) sequences and major chemical components. Boi, Seifuto, and their botanical origins overall showed seven variable sites in the ITS sequence and six genotypes. Japanese S. acutum and Boi had one nucleotide variation at position 593 to show two genotypes (J1 and J2) and their heterozygote (J3). Seifuto samples and their botanical origins, S. acutum and S. acutum var. cinereum from China, showed three genotypes (C1, C2, and C3), which did not agree with the botanical classification, indicating that they cannot be distinguished according to their ITS sequences. All Seifuto samples from Henan market showed the same ITS genotype (C1). The Japanese and Chinese genotypes differed in the nucleotide position 424, which can be used to distinguish the country of origin of these materials. In the HPLC analysis of six major components, sinomenine (1), magnoflorine (2), menisperine (3), 6-O-methyllaudanosoline glucoside (4), liriodendrin (5), and menisdaurin (6), all were detected in Boi, whereas five (all except for menisdaurin) were detected in Seifuto. The main component in the rhizome of Seifuto was sinomenine, whereas magnoflorine was the main component in the rhizome and the climbing stem of Boi. The content of sinomenine in Seifuto was almost twice that in Boi. Although the individual content of alkaloids 1-4 differed between Boi and Seifuto, the total contents of these alkaloids were comparable between them both in the climbing stem and rhizome.

Integrated Network Pharmacology and Experimental Validation Approach to Investigate the Mechanisms of Stigmasterol in the Treatment of Rheumatoid Arthritis

“…However, in recent years, more and more in-depth researches on Sinomenium acutum and the action mechanism reveal that, in addition to Sinomenine, Sinomenium acutum also contains other active compounds playing antiinflammatory and anti-bone destruction roles; for example, Stigmasterol, Magnoflorine, and Michelalbine are the active compounds contribute to the regulation of immune and inflammatory responses. 42 The exploration for other active compounds of Sinomenium acutum involving in RA treatment is critical for the investigation of the treatment mechanism of RA.…”

Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences

“…Thus, it is necessary for users to distinguish Bang Gi from other herbs. Boi and its original plant Sinomenium acutum from Japan were compared with Seifuto and its botanical origins from China in terms of their internal transcribed spacer sequences and major chemical components 14) . Based on results from BLAST searches, our sequencing result was identified in the genome sequence of Sinomenium acutum (JN051718), Stephania tetrandra (JN051727), C. trilobus (JN051704) in GenBank accession number.…”

“…Many studies have used this gene to reconstruct phylogenies or as a molecular marker within medicinal plant families [7][8][9][10][11][12][13] . A study about Bang Gi was reported by Sano et al that compared Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii) using internal transcribed spacer region sequence analysis 14) .…”