Genetic and Cytogenetic Characteristics of Pentaploidy in Water Frogs

Hermaniuk, Adam; Pruvost, Nicolas B. M.; Kierzkowski, Piotr; Ogielska, Maria

doi:10.1655/herpetologica-d-12-00037

Cited by 15 publications

(17 citation statements)

References 29 publications

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“…This was the first observation of pentaploidy within a P. esculentus hybrid complex. The detailed description of this unique individual, including erythrocyte size, DNA content, and microsatellites analysis, was published elsewhere (Hermaniuk et al 2013). The pentaploid specimen and the cross with mixed genotype of offspring were excluded from further procedures.…”

Section: Population Sources and Crossing Proceduresmentioning

confidence: 99%

Low Temperature and Polyploidy Result in Larger Cell and Body Size in an Ectothermic Vertebrate

Hermaniuk

Rybacki

Taylor

2016

Physiological and Biochemical Zoology

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Previous studies reported that low temperatures result in increases in both cell size and body size in ectotherms that may explain patterns of geographic variation of their body size across latitudinal ranges. Also, polyploidy showed the same effect on body size in invertebrates. In vertebrates, despite their having larger cells, no clear effect of polyploidy on body size has been found. This article presents the relationship between temperature, cell size, growth rate, and body size in diploid and polyploid hybridogenetic frog Pelophylax esculentus reared as tadpoles at 19° and 24°C. The size of cells was larger in both diploid and triploid tadpoles at 19°C, and triploids had larger cells at both temperatures. In diploid and triploid froglets, the temperature in which they developed as tadpoles did not affect the size of their cells, but triploids still had larger cells. Triploid tadpoles grew faster than diploids at 19°C and had larger body mass; there was no clear difference between ploidies in growth rate at 24°C. This indicates better adaptation of triploid tadpoles to cold environment. This is the first report on the increase of body mass of a polyploid vertebrate caused by low temperature, and we showed relationship between increase in cell size and increased body mass. The large body mass of triploids may provide a selective advantage, especially in colder environments, and this may explain the prevalence of triploids in the northern parts of the geographic range of P. esculentus.

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Section: Population Sources and Crossing Proceduresmentioning

confidence: 99%

Low Temperature and Polyploidy Result in Larger Cell and Body Size in an Ectothermic Vertebrate

Hermaniuk

Rybacki

Taylor

2016

Physiological and Biochemical Zoology

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“…Juveniles were analyzed separately, as we expected a higher frequency of polyploids than in adults because tetraplois and pentaploids do not survive until adulthood (Hermaniuk et al 2013, Christiansen 2009). Adult individuals (79 P. lessonae , 82 P. ridibundus and 111 P. esculentus ) were collected from 11 natural populations in Central Europe (Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…Some hybrids produce diploid gametes which give rise to triploid progeny (Uzzell et al 1975, Rybacki 1994) and the combinations of genomes provided by the mother and by the father result in two types of triploids, namely RRL and RLL. Progeny of higher ploidy (4n and 5n) were also recorded but such individuals died before completion of metamorphosis or soon after (Christiansen 2009, Hermaniuk et al 2013). …”

Section: Introductionmentioning

confidence: 99%

Variability of NOR patterns in European water frogs of different genome composition and ploidy level

Zaleśna¹,

Florek²,

Rybacki³

et al. 2017

CCG

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We studied water frogs from a complex composed of two species: Pelophylax lessonae (Camerano, 1882) (genome LL, 2n = 26) and P. ridibundus (Pallas, 1771) (RR, 2 = 26), and their natural hybrid P. esculentus (Fitzinger, 1843) of various ploidy and genome composition (RL, 2n = 26, and RRL or RLL, 3n = 39). Tetraploids RRLL were found (4n = 52) in juveniles. We applied cytogenetic techniques: AgNO3, chromomycin A3, PI and fluorescent in situ hybridization with a 28S rDNA probe. Results obtained by silver staining corresponded well with those stained with CMA3, PI and FISH. As a rule, NORs are situated on chromosomes 10. The number of Ag-NORs visible on metaphase plates was the same as the number of Ag-nucleoli present in interphase nuclei of the same individual. In all analyzed metaphases, NORs exhibited variations in size after AgNO3 and CMA3 stainings. Sixty-six individuals (out of 407 analyzed) were polymorphic for the localization and number of NORs. Fifty-one diploids had NORs only on one chromosome of pair 10. Three triploids (LLR and RRL) displayed two NORs, and two other triploid RRL individuals displayed one, instead of expected three NORs. In ten individuals extra NORs were detected on chromosomes other than 10 (chromosomes 2 and 9).

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“…This suggests that LR seems to be an essential component that can affect the survival of the other two triploid hybrids in environment. This is understandable since LR is the parental genotype of the triploid frog [6].…”

Section: (B) (C) (D) (A)mentioning

confidence: 97%

“…Pelophylax esculentus coexists in population with different ploidy levels, from diploid to pentaploid [6].…”

Section: Introductionmentioning

confidence: 99%

Coexistence analysis of diploid and triploid hybrid water frogs

Apri

Suandi

Soewono

2014

AIP Conference Proceedings

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Energy and exergy analysis of hybrid photovoltaic/thermal solar water heater considering with and without withdrawal from tank J. Renewable Sustainable Energy 2, 043106 (2010); 10.1063/1.3464754Effect of flexibility on surface tension and coexisting densities of water Abstract. A dynamical model for genotype distributions of all hybrid populations of Pelophylax esculentus in the absence of differential survival is studied here. Assuming that under natural condition the parental genotypes LL and RR do not survive into adult stage, the dynamic is then reduced into three-dimensional dynamical system of classes LR, LLR, LRR genotypes. Coexistence of diploid (LR) and triploid (LLR and LRR) genotypes is analyzed here.

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Genetic and Cytogenetic Characteristics of Pentaploidy in Water Frogs

Cited by 15 publications

References 29 publications

Low Temperature and Polyploidy Result in Larger Cell and Body Size in an Ectothermic Vertebrate

Low Temperature and Polyploidy Result in Larger Cell and Body Size in an Ectothermic Vertebrate

Variability of NOR patterns in European water frogs of different genome composition and ploidy level

Coexistence analysis of diploid and triploid hybrid water frogs

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