Genetic and kinetic analysis of Neurospora crassa mtr mutants

Larimer, Frank W.; DeBusk, A. G.

doi:10.1128/jb.129.3.1636-1638.1977

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…2000). The neutral amino acid permease is required for the uptake and transport of neutral aliphatic and aromatic amino acids from exogenous source for growth (Larimer and DeBusk 1977). The Ras 2p GTPase is a membrane‐associated guanine nucleotide‐binding protein that plays a key role in regulating cell ageing through metabolic control in yeast (review by Jazwinski 2000); Ras 2p has also been directly implicated in the regulation of invasive cell growth and of cytoskeletal polarity through the stress response pathway (Stanhill et al.…”

Section: Discussionmentioning

confidence: 99%

Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone

Cuero

Ouellet

2005

J Appl Microbiol

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treatment also affected conidial differentiation and pigmentation. A cDNA suppression subtractive library was also produced and followed by sequencing of potential metal treatment-specific clones, thus determining induced genes.The genes uncovered included enzymes and regulators of cell growth and division, including many genes with unknown functions were uncovered. A Northern blot analysis was used to verify the expression pattern of the corresponding genes under metal treatment. The metal ions enhanced the expression of alcohol dehydrogenase Adh1 homologue by up to 33-fold in A. flavus and ca fourfold in F. graminearum. Encoding homologues of a neutral amino acid permease, were also used in the Northern analysis. However, the expression of the permease was not significantly affected by metal ion treatments. Conclusions:The results showed a significant effect of metal ions on expression of gene related to fungal growth, development, conidiation and production of both aflatoxin and zearalenone. Significant and Impact of the Study: At the molecular and cellular level, the significant effects of metal ions on fungal growth and development, conidiation, and production of both aflatoxin and zearalenone were demonstrated.

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Section: Discussionmentioning

confidence: 99%

Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone

Cuero

Ouellet

2005

J Appl Microbiol

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The ftr cistron of Coprinus cinereus is the structural gene for a multifunctional transport carrier molecule

Taj-Aldeen

Moore

1982

Curr Genet

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Mutants of the basidiomycete Coprinus cinereus which were selected for their resistance to growth inhibitions caused by hexose analogues are all alleles of the ftr cistron. They are shown to have approximately normal levels of activity of enzymes involved in intracellular sugar metabolism and to accumulate normal levels of sugar phosphates. However, the mutants show greatly depressed rates of sugar uptake. Uptake rates from 0.01 mM solutions of 2-deoxy-D-glucose were only 1 to 4% of the wild type rate, and from 15 mM solutions the mutant rates were between 16 and 40% of normal. Kinetic analysis showed that the mutant Vmax values were reduced to a few per-cent of normal while Km values were relatively little changed and in some cases the mutants had an increased affinity for the substrate. Reverse mutations restored the Vmax value and the Km to about the wild type level. Previous data had shown that position of mutants within the allele map depended on selection conditions in a way that implied some interaction between the ftr gene product and the substrate. Since the mutants are defective in transport from both high and low sugar concentrations, and since they exhibit coordinated alterations in Km and Vmax, it is concluded that the ftr cistron is the structural gene for a product involved in sugar translocation (both as carrier and energisation link) in both high and low affinity glucose transport systems.

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Mutagen sensitivities and mutator effects of MMS-sensitive mutants in neurospora

Käfer

1981

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Genetic and kinetic analysis of Neurospora crassa mtr mutants

Abstract: Allelic complementation occurs at the mtr (methyltryptophan resistance) locus. Kinetic properties of neutral amino acid transport are altered for mtr mutants.

Cited by 4 publications

References 5 publications

Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone

Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone

The ftr cistron of Coprinus cinereus is the structural gene for a multifunctional transport carrier molecule

Mutagen sensitivities and mutator effects of MMS-sensitive mutants in neurospora

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