Genetic and metabolic engineering approaches for improving accessibilities of lignocellulosic biomass toward biofuels generations

Ghosh, Dipankar; Das, Somnath

doi:10.1016/b978-0-12-817953-6.00002-6

Cited by 6 publications

(3 citation statements)

References 107 publications

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“…Chemical and physicochemical treatments like organosolvent, oxidative delignification, wet oxidation, alkaline pretreatment, microwave heating with catalyst technology, natural dyes treatment, ionic liquids, acidic pretreatment, etc., can be used for delignification, but that processes are neither sustainable nor economically feasible. [7][8][9] Hence biological root is much better, having few limitations. 10 The industrial utilization of fungal enzymes is very much restricted in accordance with its genetic manipulations, protein expression level, and lower stability under high pH conditions 11 .…”

Section: Introductionmentioning

confidence: 99%

Isolation and Screening of Dye Degrading Lignocellulolytic Bacteria from Sundarban Mangrove Ecosystem, West Bengal, India

Das¹,

Sinha²,

Sen³

et al. 2023

J. Pure Appl. Microbiol.

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The present research work was carried out on isolating lignocellulolytic enzyme-producing natural bacterial isolates of the Sundarban Mangrove ecosystem in West Bengal, India. Multiple bacterial isolations and qualitative as well as quantitative screening of the isolates was conducted by selective media plates and dye decolorization assay. Growth characterization of the top three isolates, namely GD1, GD2, and GD3, was determined in both nutrient broth and selective media. GD1 showed laccase activity in ABTS plate and decolorized congo red, malachite green, and methylene blue by 64.82%, 47.69%, and 33.33%, respectively, which is the inherent property of laccase. Along with the maximum laccase activity (9.72U/L), it also showed a little amount of cellulase (5.8U/ml) and pectinase (0.55U/ml) activity in the enzyme assay. GD2 showed maximum cellulase activity (6.56U/ml) with only 3.3% degradation of congo red and 39.43% degradation of malachite green. Like GD1, GD2 also bears 5.6U/L laccase and 0.12U/ml pectinase enzyme activity. The last isolate, GD3 specialized in pectinase production and having 4.4U/ml enzyme activity, degraded congo red by 66.75% and malachite green by 54.26%.All isolates showed activity between 30° and 37°C, and pH ranges from 4.5 to 7. The outcomes of this research will be useful in the fiber industry like jute or banana, dye industry, pulp-paper industry, and textile industry for waste remediation, recycling, and fiber modification.

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Section: Introductionmentioning

confidence: 99%

Isolation and Screening of Dye Degrading Lignocellulolytic Bacteria from Sundarban Mangrove Ecosystem, West Bengal, India

Das¹,

Sinha²,

Sen³

et al. 2023

J. Pure Appl. Microbiol.

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“…Another thing is that genetic alteration in the genome of an organism modifies its substrate-binding affinity, which is determined by its Michaelis Menten constant, which is the Km value. In maximum cases, the Km value is reduced, which signifies the increased amount of substrate specificity of the enzymes (Ghosh and Das 2020). When the bacterial, fungal, and algal systems are modified, Figure1 Jute industry waste treatment, recycling and conversion positive results have been generated.…”

Section: Introductionmentioning

confidence: 99%

Potential of lignocellulolytic biocatalysts of native and proposed genetically engineered microbial cell factories on jute fiber modification and jute waste recycling: A review

Das¹,

Ghosh²

2022

J Exp Bio & Ag Sci

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The lignocellulolytic microbial systems from different parts of the world responsible for lignocellulosic biomass (LCB) like jute (Corchorus spp.) waste degradation, fiber modification, and bioenergy production are not limited to a specific prokaryotic or eukaryotic group. The industrial applications of these highly efficient bacterial, fungal and algal communities are related to the production of lignocellulolytic enzymes such as cellulase, hemicellulase, lignin-peroxidase, versatile peroxidase, laccase, thermostable oxidants, pectinase, etc. They are a blessing for the jute, dye, paper, pulp, and biofuel industries as they help to generate a sustainable ecosystem. The jute plant is lignocellulosic biomass so it can be utilized in various ways, from everyday goods to power generation. Jute industries generally use different physicochemical strategies to generate quality fiber and post-retting activities, but these approaches cannot produce desired products; hence microbial routes are best for quality fiber generation, waste remediation, and biofuel generation. To this end, this review summarizes the most important milestones of the development of the leading enzyme-producing cell factories and their engineering by genetic, metabolic, and synthetic biology approaches with the emergence of high throughput methods, such as site-directed mutagenesis and others that can analyze the relevant mutations to accelerate our understanding of lignocellulolytic enzymology.

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“…However, it fails to release the cellulose from LCW following physical, chemical, biological, physicochemical, mechanical, irradiation, extrusion, and ozonolysis pre-treatment procedures time to time. With this enzymesubstrate interaction processes ennoble the hydrolysis possibilities for upward biodiesel formation [8]. After the transformation of lignocellulosic biomass to low fermentable sugars, also another microbial catalyst has been used, for example, Zymomonas mobilis, Saccharomyces cerevisiae, Clostridium thermocellum, Clostridium phytofermentans, Trichoderma reesei, etc.…”

Section: Introductionmentioning

confidence: 99%

Mangrove algae as sustainable microbial cell factory for cellulosic biomass degradation and lipid production

Debnath¹,

Ghosh²

2022

J App Biol Biotech

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Human civilization faces a huge energy crisis due to climate alterations and reduction in fossil fuel reserves. Biofuels are sustainable platform that can combat this burning issue currently. Biodiesel is a potential fossil fuel alternative from cooking, soybean, or plant-derived oil resources. Existing biodiesel production processes are directly competing with food chain. Algae are impressive alternatives for biodiesel generation as algae grow without competition with food chain, have an inherently faster growth rate, and have higher lipid content under diverse nutrient-limiting environments. Lignocellulosic waste (LCW) accumulation has been increasing through anthropogenic activities. Accumulation of LCW causes ill effects on environment and human health. Thus, a faster paradigm shift needs to be required for establishing a single platform to perform lignocellulosic biomass degradation along with higher lipid production as biodiesel precursor. Few studies show algal endoglucanase or cellulase activities for degrading LCW to generate monomeric constituents which can further be channelized to produce higher biomass and lipid contents as biodiesel precursor. Thus, the present study focuses on a feasibility attempt to show that mangrove algal isolates could be a potential microbial cell factory for cellulose degradation as a major constituent of LCW and higher lipid production as potential precursor for biodiesel production in near future.

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Genetic and metabolic engineering approaches for improving accessibilities of lignocellulosic biomass toward biofuels generations

Cited by 6 publications

References 107 publications

Isolation and Screening of Dye Degrading Lignocellulolytic Bacteria from Sundarban Mangrove Ecosystem, West Bengal, India

Isolation and Screening of Dye Degrading Lignocellulolytic Bacteria from Sundarban Mangrove Ecosystem, West Bengal, India

Potential of lignocellulolytic biocatalysts of native and proposed genetically engineered microbial cell factories on jute fiber modification and jute waste recycling: A review

Mangrove algae as sustainable microbial cell factory for cellulosic biomass degradation and lipid production

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