Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Brown Rot in China

Wang, Li; Wang, Bingsen; Zhao, Guang‐Rong; Cai, Xingkui; Jabaji, Suha; Séguin, Philippe; Chen, Huilan

doi:10.1007/s12230-017-9576-2

Cited by 21 publications

(29 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phylotype I strains reported in China comprise 15 sequevars and phylotype II strains are consisting of 2 sequevars, see Table 1 . R. pseudosolanacearum strains possess high level of phylogenetic diversity, comprising a total of 10 sequevars (12 – 18, 34, 44, and 48) with the largest host range ( Xu et al, 2009 ; Xue et al, 2011 ; Wang L. et al, 2017 ). Recently, new sequevars were identified including sequevar 14M ( Wang L. et al, 2017 ) isolated from potato and peanut in 2015 and sequevars 54 ( Li et al, 2016 ) and 55 ( Liu et al, 2017 ) isolated from tobacco in 2016.…”

Section: Distribution and Species Complexitymentioning

confidence: 99%

Bacterial Wilt in China: History, Current Status, and Future Perspectives

et al. 2017

Self Cite

View full text Add to dashboard Cite

Bacterial wilt caused by plant pathogenic Ralstonia spp. is one of the most important diseases affecting the production of many important crops worldwide. In China, a large scientific community has been dedicated to studying bacterial wilt and its causative agent, Ralstonia pseudosolanacearum and R. solanacearum. Most of their work was published in Chinese, which has hindered international communication and collaboration in this field. In this review, we summarize the status of knowledge on geographical distribution, diversity, and host range of Ralstonia spp., as well as, the impact of bacterial wilt on important crops and disease control approaches, in China. We present areas of research and publications by Chinese scientists and propose the promotion of collaborative research within China and with the international community.

show abstract

Section: Distribution and Species Complexitymentioning

confidence: 99%

Bacterial Wilt in China: History, Current Status, and Future Perspectives

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The major pathovar infecting potato belongs to phylotype IIB, which is widely distributed worldwide (Fegan et al ., ; Hayward, ; Janse, ; Seal et al ., ). Phylotype IIB pathogens have adapted to cool temperatures and have migrated worldwide, probably through contaminated potato tubers (Hayward, ; Hooker, ; Huerta et al ., ; Wang et al ., ; Wicker et al ., ). Even though potato and R. solanacearum have a long‐term co‐evolutionary history, very little is known about the interaction between them, as a result of both a lack of resistance materials and technical barriers involving potato genetic manipulation (Chen et al ., , ).…”

Section: Introductionmentioning

confidence: 99%

A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear‐localized effector that suppresses immune responses in potato

Zheng

Wang

et al. 2019

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

Summary Both Solanum tuberosum and Ralstonia solanacearum phylotype IIB originated in South America and share a long‐term co‐evolutionary history. However, our knowledge of potato bacterial wilt pathogenesis is scarce as a result of the technical difficulties of potato plant manipulation. Thus, we established a multiple screening system (virulence screen of effector mutants in potato, growth inhibition of yeast and transient expression in Nicotiana benthamiana ) of core type III effectors (T3Es) of a major potato pathovar of phylotype IIB, to provide more research perspectives and biological tools. Using this system, we identified four effectors contributing to virulence during potato infection, with two exhibiting multiple phenotypes in two other systems, including RipAB. Further study showed that RipAB is an unknown protein with a nuclear localization signal (NLS). Furthermore, we generated a ripAB complementation strain and transgenic ripAB ‐expressing potato plants, and subsequent virulence assays confirmed that R. solanacearum requires RipAB for full virulence. Compared with wild‐type potato, transcriptomic analysis of transgenic ripAB ‐expressing potato plants showed a significant down‐regulation of Ca 2+ signalling‐related genes in the enriched Plant–Pathogen Interaction (PPI) gene ontology (GO) term. We further verified that, during infection, RipAB is required for the down‐regulation of four Ca 2+ sensors, Stcml5 , Stcml23 , Stcml‐cast and Stcdpk2 , and a Ca 2+ transporter, Stcngc1 . Further evidence showed that the immune‐associated reactive oxygen species (ROS) burst is attenuated in ripAB transgenic potato plants. In conclusion, a systematic screen of conserved R. solanacearum effectors revealed an important role for RipAB, which interferes with Ca 2+ ‐dependent gene expression to promote disease development in potato.

show abstract

“…But the dominant strain is different in different regions. For example, phylotype I and phylotype IV strains are dominant in Japan and Korea and phylotype IIB-1 strains are dominant in China (Xu et al, 2009; Cellier and Prior, 2010; Sagar et al, 2013; Wang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, our group has identified a R. solanacearum strain HA4-1, also addressed as PeaHuB4, to be phylotype I, sequevar 14M and biovar 3 (Wang et al, 2017). HA4-1 is shown to be highly virulent in most given potato species, while hypovirulent, even avirulent in other potato species.…”

Section: Introductionmentioning

confidence: 99%

“…HA4-1 is shown to be highly virulent in most given potato species, while hypovirulent, even avirulent in other potato species. Notably, HA4-1 showed contrasting virulence in certain potato species when compared with GMI1000 and UW551, the latter being the major pathovars infecting potato in most regions (Hayward, 1991; Fegan and Prior, 2005; Xu et al, 2009; Janse, 2010; Wang et al, 2017). To dissect the differential pathogenesis of HA4-1 and other strains, we sequenced and analyzed the HA4-1 genome by combined strategies including Illumina HiSeq2000, PacBio RS II platform and genomic BAC-end sequencing.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Complete Genome Sequence of Sequevar 14M Ralstonia solanacearum Strain HA4-1 Reveals Novel Type III Effectors Acquired Through Horizontal Gene Transfer

Tan

Qiu

et al. 2019

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Ralstonia solanacearum , which causes bacterial wilt in a broad range of plants, is considered a “species complex” due to its significant genetic diversity. Recently, we have isolated a new R. solanacearum strain HA4-1 from Hong’an county in Hubei province of China and identified it being phylotype I, sequevar 14M (phylotype I-14M). Interestingly, we found that it can cause various disease symptoms among different potato genotypes and display different pathogenic behavior compared to a phylogenetically related strain, GMI1000. To dissect the pathogenic mechanisms of HA4-1, we sequenced its whole genome by combined sequencing technologies including Illumina HiSeq2000, PacBio RS II, and BAC-end sequencing. Genome assembly results revealed the presence of a conventional chromosome, a megaplasmid as well as a 143 kb plasmid in HA4-1. Comparative genome analysis between HA4-1 and GMI1000 shows high conservation of the general virulence factors such as secretion systems, motility, exopolysaccharides (EPS), and key regulatory factors, but significant variation in the repertoire and structure of type III effectors, which could be the determinants of their differential pathogenesis in certain potato species or genotypes. We have identified two novel type III effectors that were probably acquired through horizontal gene transfer (HGT). These novel R. solanacearum effectors display homology to several YopJ and XopAC family members. We named them as RipBR and RipBS. Notably, the copy of RipBR on the plasmid is a pseudogene, while the other on the megaplasmid is normal. For RipBS, there are three copies located in the megaplasmid and plasmid, respectively. Our results have not only enriched the genome information on R. solanacearum species complex by sequencing the first sequevar 14M strain and the largest plasmid reported in R. solanacearum to date but also revealed the variation in the repertoire of type III effectors. This will greatly contribute to the future studies on the pathogenic evolution, host adaptation, and interaction between R. solanacearum and potato.

show abstract

Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Brown Rot in China

Cited by 21 publications

References 28 publications

Bacterial Wilt in China: History, Current Status, and Future Perspectives

Bacterial Wilt in China: History, Current Status, and Future Perspectives

A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear‐localized effector that suppresses immune responses in potato

Complete Genome Sequence of Sequevar 14M Ralstonia solanacearum Strain HA4-1 Reveals Novel Type III Effectors Acquired Through Horizontal Gene Transfer

Contact Info

Product

Resources

About