Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species

Abstract

Fusarium proliferatum (Matsushima) Nirenberg is a common pathogen infecting numerous crop plants and occurring in various climatic zones. It produces large amounts of fumonisins, a group of polyketide-derived mycotoxins. Fumonisin biosynthesis is determined by the presence and activity of the FUM cluster, several co-regulated genes with a common expression pattern. In the present work, we analyzed 38 F. proliferatum isolates from different host plant species, demonstrating host-specific polymorphisms in partial sequences of the key FUM1 gene (encoding polyketide synthase). We also studied growth rates across different temperatures and sample origin and tried to establish the relationships between DNA sequence polymorphism and toxigenic potential. Phylogenetic analysis was conducted based on FUM1 and tef-1α sequences for all isolates. The results indicated the greatest variations of both toxigenic potential and growth patterns found across the wide selection of isolates derived from maize. Fumonisin production for maize isolates ranged from 3.74 to 4,500 μg/g of fumonisin B1. The most efficient producer isolates obtained from other host plants were only able to synthesize 1,820–2,419 μg/g of this metabolite. A weak negative rank correlation between fumonisin content and isolate growth rates was observed. All garlic-derived isolates formed a distinct group on a FUM1-based dendrogram. A second clade consisted of tropical and sub-tropical strains (isolated from pineapple and date palm). Interestingly, isolates with the fastest growth patterns were also grouped together and included both isolates originating from rice. The sequence of the FUM1 gene was found to be useful in revealing the intraspecific polymorphism, which is, to some extent, specifically correlated with the host plant.Electronic supplementary materialThe online version of this article (doi:10.1007/s13353-011-0059-8) contains supplementary material, which is available to authorized users.

Population Structure of the Gibberella fujikuroi Species Complex Associated with Rice and Corn in Korea

“…Additionally, the presence of ricespecific subclades of F. fujikuroi and F. proliferatum in trees may indicate that these isolates have a long evolutionary relationship with rice in Korea; those isolated from corn were possibly introduced into Korea more recently than rice isolates. This may be comparable to the presence of host-specific polymorphisms of F. proliferatum isolates from different host plants in partial sequences of FUM1 (Stepien et al, 2011). However, a comprehensive understanding of genetic diversity and population structures of these species from both crops requires further investigation, such as the determination of effective population size and genetic exchanges of multiloci using more field isolates obtained over longer time periods.…”

Formation of 6-n-pentyl-2H-pyran-2-one (6-PAP) and other volatiles by different Trichoderma species

“…These strains were identified on the basis of morphological, molecular, and biochemical (mycotoxin production) analyses by Stępień et al (2008Stępień et al ( , 2011.…”

The effects of storage duration, temperature and cultivar on the severity of garlic clove rot caused by Fusarium proliferatum

“…(Dissanayake et al, 2009;Garcia et al, 2011;Stepien et al, 2011). Moreover, the presence of F. proliferatum has been reported in garlic in other countries (Dugan et al, 2007;Ravi and Prasand, 2012) as well as its ability to produce fumonisin in garlic in Germany (Seefelder et al, 2002).…”

“…Moreover, F. proliferatum produces a number of toxins apart from fumonisins, such as moniliformin (Marasas et al, 1984), beauvericin (Logrieco et al, 1998;Plattner and Nelson, 1994), fusaric acid (FA) (Bacon et al, 1996) and fusaroproliferin (Ritieni et al, 1995). Because fresh garlic is consumed broadly in many countries, the production of mycotoxins in cloves infected with F. proliferatum must not be disregarded (Palmero et al, 2010b;Stankovic et al, 2007;Stepien et al, 2011).…”