Genetic and phylogenetic analysis of feline calicivirus isolates in China

Sun, Yuan; Deng, Mingliang; Peng, Zhong; Hu, Ruiming; Chen, Huanchun; Wu, Bin

doi:10.1016/j.tvjl.2016.12.014

Cited by 25 publications

(40 citation statements)

References 9 publications

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“…The phylogenetic analysis of this study showed two clusters (I and II) with a significant aLTR value for the variable region and isolates from this study and the most of Brazilian isolated were close related with FCV genogroup I. As previously reported by, 36 Chinese and Japanese isolates formed a single subgroup in genogroup II probably due the geographical proximity between these two countries. 36 …”

Section: Discussionsupporting

confidence: 81%

“…As previously reported by, 36 Chinese and Japanese isolates formed a single subgroup in genogroup II probably due the geographical proximity between these two countries. 36 …”

Section: Discussionsupporting

confidence: 78%

“…FCV is considered to belong to a single antigenically diverse serotype 34 and, so far, many studies have tried to classify the different variants based on hypervariable region E of the genome. Sato et al 35 and Sun et al 36 have proposed a classification dividing FCV isolates into two distinct genogroups based on phylogenetic analyses of capsid gene. Genogroup I include global isolates and genogroup II includes only isolates from Japan and China.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Pereira

Baumworcel

Fioretti

et al. 2018

Brazilian Journal of Microbiology

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The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic. Feline calicivirus were isolated in CRFK cells and characterized by reverse transcription PCR target to both conserved and variable regions of open reading frame 2. The amplicons obtained were sequenced. A phylogenetic analysis along with most of the prototypes available in GenBank database and an amino acid analysis were performed. Phylogenetic analysis based on both conserved and variable region revealed two clusters with an aLTR value of 1.00 and 0.98 respectively and the variants from this study belong to feline calicivirus genogroup I. No association between geographical distribution and/or clinical signs and clustering in phylogenetic tree was observed. The variants circulating in public short-term animal shelter demonstrated a high variability because of the relatively rapid turnover of carrier cats constantly introduced of multiple viruses into this location over time.

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Section: Discussionsupporting

confidence: 81%

“…As previously reported by, 36 Chinese and Japanese isolates formed a single subgroup in genogroup II probably due the geographical proximity between these two countries. 36 …”

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Pereira

Baumworcel

Fioretti

et al. 2018

Brazilian Journal of Microbiology

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“…The E region contains the major B cell epitopes and plays an important role in the formation of viral particle antigen structures. And conformational epitopes are probably more important than linear epitopes in viral neutralization (Radford et al 2007;Sun et al 2017). VP2 is essential for productive replication that results in the synthesis and maturation of infectious virions (Brunet et al 2019;Sosnovtsev et al 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the persistent surveillance should be performed for the prevalence of FCV isolates and can provide an important insight into the development of novel vaccines. In recent years, there have been some reports of Chinese FCV strains, but few reports of FCV strains from the Chinese stray cats were reported (Guo et al 2018;Sun et al 2017). In view of the larger sphere of activity for stray cats, it is easier to spread viruses.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of a Natural Mutant of Feline Calicivirus in China

Li¹,

Chen²,

Chen³

et al. 2021

Preprint

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During epidemiological surveillance of Feline calicivirus (FCV) isolates in Shanghai, China, a natural mutant of FCV, designated SH1909, was successfully isolated from a stray cat. The complete genome sequence of SH1909 was determined in this study. Sequence comparison and analysis showed that thirteen unique aa residues substitutions and single-aa insertion of N or Y were observed in SH1909, which indicated that SH1909 was a novel and natural mutant of FCV. Interestingly, phylogenetic analysis based on LC-VP1 showed SH1909 could be clustered into an independent evolutionary branch with some Chinese isolates and was more distantly related to vaccine strains, indicating its potential to escape from vaccine-elicited immunity. According to the predicted B-cell epitopes of LC-VP1, amino acid mutation sites and positive selective sites, peptide C (aa sites: 445–460) and, peptide D (aa sites: 425–440) located in the hypervariable regions of LC-VP1, may result in the decreased immunological protection. Moreover, amino acid sites 439 and 449 may be responsible for the potential immune escape of SH1909. This study provides an important insight into genetic variations of FCV and vaccine development.

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Isolation and phylogenetic analysis of three feline calicivirus strains from domestic cats in Jilin Province, China

et al. 2017

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Feline calicivirus (FCV) is a highly prevalent pathogen that can cause infectious felid upper respiratory tract disease. The majority of complete genome sequences of FCV strains reported to date are from the USA. In this study, three FCV strains, CH-JL1, CH-JL2 and CH-JL3, were isolated from domestic cats in Jilin Province, China. Sequence analysis revealed that except for strains HRB-SS, WZ-1, XH, 12Q087-1 and 12Q087-5, the 3' untranslated regions (UTRs) of CH-JL2 and CH-JL3 are more than 20 nucleotides longer than those of all other reference isolates. The complete sequences of the three CH-JLs were compared with other reference strains, with nucleotide sequence identity values in the range of 76.2%-82.2%, 76.8%-96.4 and 76.8%-96.4%. Phylogenetic analysis showed that CH-JL1 forms a branch with FB-NJ-13, GD, 12Q087-1 and 12Q087-5. CH-JL2 was found to be most closely related to CH-JL3, forming another branch together with the other isolates. CH-JL1 shares a long nucleotide span with CH-JL2 and CH-JL3. It can be inferred that many FCV strains are co-circulating in Jilin Province. The availability of complete genome sequences will serve as a reference for future epidemiological studies of FCV.

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Genetic and phylogenetic analysis of feline calicivirus isolates in China

Cited by 25 publications

References 9 publications

Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Molecular Characterization of a Natural Mutant of Feline Calicivirus in China

Isolation and phylogenetic analysis of three feline calicivirus strains from domestic cats in Jilin Province, China

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