Genetic and Sequence Analysis of Markers Tightly Linked to the Resistance Gene, 3

Abstract

Soybean mosaic virus (SMV) is a major viral pathogen, affecting soybean [Glycine max (L.) Merr.] production worldwide. The Rsv3 gene of soybean confers resistance to three of the most virulent strains (G5–G7) of SMV. The objectives of this study were to map Rsv3 and develop polymerase chain reaction (PCR) based markers for marker‐assisted selection (MAS) purposes. Disease‐response data were collected from two F2 mapping populations, L29 (Rsv3) × Lee68 (rsv3) and Tousan 140 (Rsv3) × Lee68 (rsv3). Bulk segregant analysis based on amplified fragment length polymorphism (AFLP) markers demonstrated that the Rsv3 locus maps to the soybean molecular linkage group (MLG) B2 between restriction fragment length polymorphism (RFLP) markers A519 and Mng247. These two tightly linked RFLP markers were converted to PCR‐based markers to expedite MAS. Sequence analysis of the Mng247 genomic region revealed similarity to the consensus sequence of a leucine‐rich repeat (LRR) characteristic of the extracellular LRR class of disease resistance genes. Results from this study will be useful in pyramiding viral resistance genes and in cloning the Rsv3 gene.

Mapping of SMV resistance gene Rsc-7 by SSR markers in soybean

“…In addition, mapping of these resistance genes has been reported. Yu et al (1994) found that two RFLP markers (PA186 and PK644a) and one SSR marker (SM176) were closely linked to Rsv1, on the molecular linkage group (MLG) F. Subsequently, Rsv3 and Rsv4 were mapped to MLG B2 (Jeong et al, 2002) and MLG D1b+W (Hayes et al, 2000), respectively. These molecular markers identified for SMV resistance genes could have the potential to facilitate both marker-assisted selection (MAS) and map-based cloning of resistance genes.…”

Tropical Food Legumes

“…Pyramiding viral resistance genes against SbMV is reported to be benefited by gene mapping and markerassisted selection (MAS) with PCR-based markers for the Rsv3 gene conferring resistance to three of the most virulent strains G5, G6, and G7 of SbMV ( Jeong et al, 2002). Molecular markers of three resistance genes have been developed based on mapping with several molecular techniques such as restriction fragment length polymorphism, random amplified polymorphism DNA, amplified fragment length polymorphism (AFLP), simple sequence repeats (SSRs), and single nucleotide polymorphisms (SNPs) to assist in plant breeding programs (e.g., Hwang et al, 2006;Shi et al, 2009Shi et al, , 2011Wang et al, 2011).…”

Marker-assisted identification of resistance genes to soybean mosaic virus in soybean lines

“…Although Hwangkeumkong (Suwon 97) displayed immunity to the G1 to G7 SMV strains, the G5H, G6H, and G7H strains induced necrotic symptoms in this cultivar. The L29 soybean line, which carries Rsv3 as its sole SMV resistance gene (Buss et al 1999;Jeong et al 2002), showed mosaic symptoms in reaction to infection with the G1, G2, G3, G4, G7H, and G7a strains, and was resistant to the G5, G5H, G6, G6H, and G7 strains. The V94-5152 soybean line, which carries Rsv4 as its sole SMV resistance gene (Buss et al 1997), was resistant to all SMV strains ( Table 2).…”

“…The presence of the Rsv4 gene results in completely dominant resistance to seven SMV strains, masking the expression of the systemic necrosis resulting from the expression of the Rsv1 allele (Ma et al 1995). The availability of a single resistance gene-containing line from the inheritance studies has led to the molecular mapping of Rsv1, Rsv3, and Rsv4 on Chromosome (Chr) 13 (linkage groups (LGs) F), 14 (LG B2), and 2 (LG D1b), respectively (Yu et al 1994;Hayes et al 2000Hayes et al , 2004Jeong et al 2002).…”