Genetic architecture of a wing size measure in Drosophila hibisci from two populations in eastern Australia

Wolf, Larry L.; Starmer, William T.; Polák, Michal; Barker, J. S. F.

doi:10.1046/j.1365-2540.2000.00763.x

Cited by 11 publications

(5 citation statements)

References 36 publications

(33 reference statements)

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“…Because the BW of MIP>TNT flies immediately after eclosion (day 0) was not different from that of controls, it was unlikely that silencing MIP neurons increased BW simply by promoting growth during development. Consistent with this idea, there was no difference between MIP>TNT and controls in the body length [13,14] and wing length [15] shortly after eclosion (Figures S1C and S1D). Furthermore, we examined whether adultspecific silencing of MIP neurons increases BW by combining tub-GAL80 ts that encodes a ubiquitously expressing temperature-sensitive GAL80 [16] with either MIP>TNT or MIP>impTNT.…”

Section: Resultssupporting

confidence: 72%

Identification of a Peptidergic Pathway Critical to Satiety Responses in Drosophila

et al. 2016

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Although several neural pathways have been implicated in feeding behaviors in mammals [1-7], it remains unclear how the brain coordinates feeding motivations to maintain a constant body weight (BW). Here, we identified a neuropeptide pathway important for the satiety and BW control in Drosophila. Silencing of myoinhibitory peptide (MIP) neurons significantly increased BW through augmented food intake and fat storage. Likewise, the loss-of-function mutation of mip also increased feeding and BW. Suppressing the MIP pathway induced satiated flies to behave like starved ones, with elevated sensitivity toward food. Conversely, activating MIP neurons greatly decreased food intake and BW and markedly blunted the sensitivity of starved flies toward food. Upon terminating the activation protocol of MIP neurons, the decreased BW reverts rapidly to the normal level through a strong feeding rebound, indicating the switch-like role of MIP pathway in feeding. Surprisingly, the MIP-mediated BW decrease occurred independently of sex peptide receptor (SPR), the only known receptor for MIP, suggesting the presence of a yet-unknown MIP receptor. Together, our results reveal a novel anorexigenic pathway that controls satiety in Drosophila and provide a new avenue to study how the brain actively maintains a constant BW.

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Section: Resultssupporting

confidence: 72%

Identification of a Peptidergic Pathway Critical to Satiety Responses in Drosophila

et al. 2016

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“…If recessive mutations accumulate at a rate higher in laboratory culture than they do in wild populations, we would expect dominance effects to be more apparent in populations that have been in laboratory culture for longer periods of time. An emerging pattern seems to agree with this prediction; there is a higher incidence of dominance in line‐cross studies involving long established laboratory populations (Gilchrist and Partridge 1999; Kennington et al 2001) compared to those based on freshly collected populations (Wolf et al 2000; Schiffer et al 2006; this study).…”

Section: Discussionsupporting

confidence: 69%

THE GENETIC ARCHITECTURE OF WING SIZE DIVERGENCE AT VARYING SPATIAL SCALES ALONG A BODY SIZE CLINE INDROSOPHILA MELANOGASTER

Kennington

Hoffmann

2010

Evolution

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Latitudinal clines in quantitative traits are common, but surprisingly little is known about the genetic bases of these divergences and how they vary within and between clines. Here, we use line-cross analysis to investigate the genetic architecture of wing size divergences at varying spatial scales along a body size cline in Drosophila melanogaster. Our results revealed that divergences in wing size along the cline were due to strong additive effects. Significant nonadditive genetic effects, including epistasis and maternal effects, were also detected, but they were relatively minor in comparison to the additive effects and none were common to all crosses. There was no evidence of increased epistasis in crosses between more geographically distant populations and, unlike in previous studies, we found no significant dominance effects on wing size in any cross. Our results suggest there is little variation in the genetic control of wing size along the length of the Australian cline. They also highlight marked inconsistencies in the magnitude of dominance effects across studies, which may reflect different opportunities for mutation accumulation while lines are in laboratory culture.

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“…To avoid recording residence times that are unlikely to be due to flies being arrested there by properties of the patch, the residence time was only established if the patch visit lasted for at least 30 s. As soon as the flies left the patch they were removed from the vials and put into fresh tubes inside an ice basin. A wing was removed from paralyzed individuals under the stereomicroscope and the length was measured and used as a proxy for their body size (Figure 2 ; Wolf et al, 2000 ). Then, flies were individually crushed in 1 mL saline (NaCl: 0.8%, Tween‐80: 0.01%) with a pestle in 2 mL tubes to collect yeast cells in/on the body as a proxy to yeast load of a fly (yeast load).…”

Section: Methodsmentioning

confidence: 99%

Transmission of beneficial yeasts accompanies offspring production in Drosophila—An initial evolutionary stage of insect maternal care through manipulation of microbial load?

Cho

Rohlfs

2023

Ecology and Evolution

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Parent‐to‐offspring transmission of beneficial microorganisms is intimately interwoven with the evolution of social behaviors. Ancestral stages of complex sociality–microbe vectoring interrelationships may be characterized by high costs of intensive parental care and hence only a weak link between the transmission of microbial symbionts and offspring production. We investigate the relationship between yeast symbiont transmission and egg‐laying, as well as some general factors thought to drive the “farming” of microscopic fungi by the fruit fly Drosophila melanogaster, an insect with no obvious parental care but which is highly dependent on dietary microbes during offspring development. The process of transmitting microbes involves flies ingesting microbes from their previous environment, storing and vectoring them, and finally depositing them to a new environment. This study revealed that fecal materials of adult flies play a significant role in this process, as they contain viable yeast cells that support larval development. During single patch visits, egg‐laying female flies transmitted more yeast cells than non‐egg‐laying females, suggesting that dietary symbiont transmission is not random, but linked to offspring production. The crop, an extension of the foregut, was identified as an organ capable of storing viable yeast cells during travel between egg‐laying sites. However, the amount of yeast in the crop reduced rapidly during periods of starvation. Although females starved for 24 h deposited a smaller amount of yeast than those starved for 6 h, the yeast inoculum produced still promoted the development of larval offspring. The results of these experiments suggest that female Drosophila fruit flies have the ability to store and regulate the transfer of microorganisms beneficial to their offspring via the shedding of fecal material. We argue that our observation may represent an initial evolutionary stage of maternal care through the manipulation of microbial load, from which more specialized feedbacks of sociality and microbe management may evolve.

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Genetic architecture of a wing size measure in Drosophila hibisci from two populations in eastern Australia

Cited by 11 publications

References 36 publications

Identification of a Peptidergic Pathway Critical to Satiety Responses in Drosophila

Identification of a Peptidergic Pathway Critical to Satiety Responses in Drosophila

THE GENETIC ARCHITECTURE OF WING SIZE DIVERGENCE AT VARYING SPATIAL SCALES ALONG A BODY SIZE CLINE INDROSOPHILA MELANOGASTER

Transmission of beneficial yeasts accompanies offspring production in Drosophila—An initial evolutionary stage of insect maternal care through manipulation of microbial load?

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