Genetic association of interleukin 18 (-607C/A, rs1946518) single nucleotide polymorphism with asthmatic children, disease severity and total IgE serum level

Ezzat, Dina Ahmed; Morgan, Dalia Saber; Mohamed, Rabab A; Mohamed, Asmaa

doi:10.5114/ceji.2019.89603

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…40 In addition, the contents of IL-4, IL-5, and IL-13 were increased in the sera of asthma patients. 41 The present study showed that the number of total cells, that is, neutrophils, eosinophils, macrophages, and lymphocytes, was significantly increased in OVA-treated mice, similar to the results reported by Zhang et al 42 Moreover, OVA induced a prominent upregulation in the contents of IL-4, IL-5, and IL-13 in BALF, consistent with the previous reports. 24,42 However, these effects were counteracted with the treatment of aloin.…”

Section: Discussionsupporting

confidence: 93%

Protective effects of aloin on asthmatic mice by activating Nrf2/HO-1 pathway and inhibiting TGF-β/Smad2/3 pathway

Xia

Yang

et al. 2023

Allergol Immunopathol

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Background: Asthma is a severe chronic respiratory disease affecting all age groups with increasing prevalence. Anti-inflammatory strategies are promising options for the treatment of asthma. Although the inhibitory effect of aloin on inflammation has been demonstrated in various diseases, its effect on asthma remains unknown. Methods: A mice asthma model was established by treating with ovalbumin (OVA). The effects and mechanism of aloin on the OVA-treated mice were determined by enzyme-linked-immunosorbent serologic assay, biochemical examination, hematoxylin and eosin and Masson's staining, and Western blot assay. Results: OVA treatment in mice significantly increased the number of total cells, neutrophils, eosinophils, and macrophages and the concentration of interleukin (IL)-4, IL-5, and IL-13, which were attenuated with the administration of aloin. The content of malondialdehyde was enhanced in OVA-treated mice, with the decreased levels of superoxide dismutase and glutathione, which were reversed with aloin treatment. Aloin treatment reduced the airway resistance of OVA-induced mice. The inflammatory cell infiltration around small airways was accompanied by the thickening and contraction of bronchial walls and pulmonary collagen deposition in OVA-treated mice; however, these conditions were ameliorated with aloin treatment. Mechanically, aloin upregulated the expression of nuclear factor erythroid 2-related fac-tor 2 (Nrf2)—heme oxygenase 1 (HO-1) pathway but inhibited the level of transforming growth factor beta–SMAD2/3 genes (TGF-β/Smad2/3) axis in OVA-induced mice. Conclusion: Aloin treatment lessened airway hyperresponsiveness, airway remodeling, inflammation, and oxidative stress in OVA-treated mice, and was closely related to the activation of Nrf2/HO-1 pathway and the weakening of TGF-β/Smad2/3 pathway.

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Section: Discussionsupporting

confidence: 93%

Protective effects of aloin on asthmatic mice by activating Nrf2/HO-1 pathway and inhibiting TGF-β/Smad2/3 pathway

Xia

Yang

et al. 2023

Allergol Immunopathol

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“…In addition, the inflammation reaction of asthma is mainly related to IgE-mediated type I allergic reaction; therefore, the secretion of IgE and related inflammation-related factors (IL-4, IL-5, IL-13) is up-regulated in the serum of asthma patients. 31 We then further detected the OVA-IgE and the secretion of these factors. The results confirmed that curcumol mitigated the up-regulated serum OVA-IgE, IL-4, IL-5, and IL-13 in chronic asthmatic mice.…”

Section: Discussionmentioning

confidence: 99%

Curcumol Ameliorates Lung Inflammation and Airway Remodeling via Inhibiting the Abnormal Activation of the Wnt/β-Catenin Pathway in Chronic Asthmatic Mice

Jia¹,

Guo²,

Lü³

et al. 2021

DDDT

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Background Curcumol exhibits anti-inflammatory effect, but its effect on chronic asthma lacked research. Therefore, this study explored the role of curcumol in asthma. Methods A chronic asthmatic mice model was established by ovalbumin induction. After treatment with curcumol, airway resistance in mice was detected by forced oscillation technique. The histopathological features of airway tissues, pulmonary inflammation, and inflammation cell recruitment in the bronchoalveolar lavage fluid (BALF) of mice were detected by hematoxylin-eosin staining. Collagen deposition in the airways of mice was examined by Masson staining. The secretion of ovalbumin-IgE, IL-4, IL-5, IL-13 in mouse serum and VEGFA secretion in BALF were analyzed by ELISA. Finally, the expressions of β-catenin, Wnt5a, VEGFA, TGF-β1, Fibronectin, and MMP-9 in mice lung tissues were determined by Western blot or immunohistochemical. Results Curcumol attenuated airway hyperresponsiveness, airway remodeling, and pulmonary inflammation in chronic asthmatic mice. Curcumol relieved collagen deposition in airway tissues, inflammation cell recruitment in BALF, and reduced the up-regulation of serum ovalbumin-IgE, IL-4, IL-5, and IL-13 and BALF VEGFA in chronic asthmatic mice. In addition, curcumol attenuated the up-regulated expressions of β-catenin, Wnt5a, VEGFA, TGF-β1, Fibronectin, and MMP-9 in the lung tissues of chronic asthmatic mice, but curcumol treatment did not show such effects on healthy mice. Conclusion Our findings revealed that curcumol could ameliorate lung inflammation and airway remodeling by inhibiting the abnormal activation of the Wnt/β-catenin pathway in chronic asthmatic mice, indicating that curcumol could be used as a novel anti-asthma drug for basic and clinical research.

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“…Further on, these sufferers with the TT/TG genotype for this SNP were linked to a higher risk of asthma (OR = 6.417 and CI: 2.432-17.289). Moreover, incidence of the heterozygous GT genotype and T allele in this SNP was higher in asthmatic kids, and there was also no relationship between the intensity of asthma and this variant [40].…”

Section: Experimental Standpointmentioning

confidence: 87%

IL-18 and CD14 variants in chronic HBV predisposition: an experimental–bioinformatics study focused on transcription and splicing

Sarhadi

Pahlavani

Razavi

et al. 2022

Preprint

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Background Hepatitis B virus (HBV), a vaccine-avoidable infection, is a health concern worldwide, leading to liver disorders such as acute self-constraint and chronic hepatitis, liver failure, hepatic cirrhosis, and even hepatocellular carcinoma if untreated. ‘Immunogeneticprofiling,’ genetic variations of the pro- and anti-inflammatory cytokines responsible for regulating the immune responses, cause person-to-person differences and impact the clinical manifestation of the disease. The current experimental–bioinformatics research was conducted to examine whether promoteric IL-18–rs187238 C > G and –rs1946518 T > G and intronic CD14–rs2569190 A > G variations are associated with chronic HBV. Methods A total of 400 individuals (200 in each case and control group) participated in the study and were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The data was also assessed bioinformatics-wise for conservation, genomic transcription and splicing, and protein interactions. Results Findings proposed that unlike the IL-18–rs1946518 T > G and CD14–rs2569190 A > G, the IL-18–rs187238 C > G is a protector against chronic HBV (odds ratio [OR] = 0.62, 95% confidence intervals [CI]: 0.46–0.83, and p = 0.002). The TG/CC/AA, TG/CC/AG, TT/CC/AG, and GG/CC/AA combined genotypes significantly increased chronic HBV risk (p < 0.05), while the IL-18 G/T and G/G haplotypes lessened it (p < 0.05). Moreover, in contrast to the IL-18–rs1946518 T > G, IL-18–rs187238 C > G is likely to create novel binding sites for transcription factors, and the CD14–rs2569190 A > G presumably changed the ribonucleic acid splicing pattern. Conclusions The IL-18–rs187238 C > G might protect against chronic HBV and is likely to generate novel binding sites for transcription factors.

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Genetic association of interleukin 18 (-607C/A, rs1946518) single nucleotide polymorphism with asthmatic children, disease severity and total IgE serum level

Cited by 5 publications

References 22 publications

Protective effects of aloin on asthmatic mice by activating Nrf2/HO-1 pathway and inhibiting TGF-β/Smad2/3 pathway

Protective effects of aloin on asthmatic mice by activating Nrf2/HO-1 pathway and inhibiting TGF-β/Smad2/3 pathway

Curcumol Ameliorates Lung Inflammation and Airway Remodeling via Inhibiting the Abnormal Activation of the Wnt/β-Catenin Pathway in Chronic Asthmatic Mice

IL-18 and CD14 variants in chronic HBV predisposition: an experimental–bioinformatics study focused on transcription and splicing

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