Genetic Basis of Resistance to Trauma in Inbred Strains of Mice

Radojicić, C; Andrič, B.; Simovic, Misho O.; Dujić, A; Marinković, Darko

doi:10.1097/00005373-199002000-00014

Cited by 14 publications

(6 citation statements)

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“…In contrast to T helper (Th) 2-dominant BALB/c mice, C57BL/6 mice are Th1-dominant and more resistant to trauma-induced sepsis (35) and Th1-dependent pathological infections, such as Leishmania major (36, 37). Thus, female C57BL/6 mice were used extensively as an animal of choice for inducing experimental sepsis.…”

Section: Notesmentioning

confidence: 99%

Sepsis Induced by Cecal Ligation and Puncture

Wen

2013

Methods in Molecular Biology

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Summary Despite advances in intensive care unit interventions, including the use of specific antibiotics and anti-inflammation treatment, sepsis with concomitant multiple organ failure is the most common cause of death in many acute care units. In order to understand the mechanisms of clinical sepsis and develop effective therapeutic modalities, there is a need to use effective experimental models that faithfully replicate what occurs in patients with sepsis. Several models are commonly used to study sepsis, including intravenous endotoxin challenge, injection of live organisms into the peritoneal cavity, establishing abscesses in the extremities, and the induction of polymicrobial peritonitis via cecal ligation and puncture (CLP). Here, we describe the surgery procedure of CLP in mice, which has been proposed to closely replicate the nature and course of clinical sepsis in humans.

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Section: Notesmentioning

confidence: 99%

Sepsis Induced by Cecal Ligation and Puncture

Wen

2013

Methods in Molecular Biology

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“…Previous studies have shown that survival and wound-healing rates vary significantly depending on strain, gender, and age, 16–19 and genetic differences may account for some of the variations observed. 17 In these studies, however, there was little information on the impact of these factors on the basic metrics of the hypermetabolic response (resting energy expenditure, heart rate, and core body temperature). This information could substantially facilitate a better understanding of the role of hypermetabolism on postburn survival and subsequently enable optimized models and treatment strategies.…”

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confidence: 99%

Effects of Burn Injury on Markers of Hypermetabolism in Rats

Izamis

Uygun

et al. 2009

Journal of Burn Care & Research

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The basic metrics of hypermetabolism have not been thoroughly characterized in rat burn injury models. We examined three models expected to differ in sensitivity to burn injury to identify that which group(s) exhibited the most clinically relevant metabolic response. Six and 12 weeks old male CD (6 week mCD and 12 week mCD) rats, and 12 weeks old female Fischer (12 week fFI) rats received a 20% total body surface area burn, followed by saline resuscitation. Activity, core body temperature, heart rate (via implanted telemetry devices), body weight, food and water intake, and fecal output were measured daily for 1 week before and after burn. Rats lost weight initially postburn but resumed weight gain by 1 week, except for 12 week mCD rats. Core body temperature increased above normal 2 days postburn and returned to baseline by 1 week. Food intake, normalized to body weight, remained unchanged postburn for 12 week mCD rats, but decreased in 6 week mCD rats and increased in 12 week fFI rats. Heart rate in the 12 week mCD and 12 week fFI rats remained at 10 to 15% above baseline, whereas, in 6 week mCD, heart rates returned to baseline after 4 days. Activity levels were unchanged for 12 week fFI and 6 week mCD rats postburn, but decreased for 12 week mCD rats. Postburn hypermetabolism was most significant and sustained in 12 week mCD rats, of least consequence and brief in 6 week mCD rats, and intermediate in 12 week fFI rats. The disparate responses indicate that the choice of animal model should be carefully considered in hypermetabolism studies.

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“…However, C57BL/6 mice have relative tendency to express Th1 phenotype [25]. BALB/c mice were clearly more sensitive to applied different trauma than C57BL/6 mice and this was correlated with the degree of genetic difference between two strains [26]. The Th2 phenotype produces relatively more anti-inflammatory cytokines, including IL-10 than pro-inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%