Genetic basis of resistance to<i>Pseudomonas syringae</i>pv.<i>tomato</i>in field tomatoes

Pitblado, R.E.; MacNeill, B.H.

doi:10.1080/07060668309501606

Cited by 42 publications

(21 citation statements)

References 9 publications

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“…This made it important to confirm that the susceptible individuals were derived from 76R. We scored DNA from the susceptible plants for restriction fragment length polymorphisms (RFLPs) specific to 76R; these RFLPs exist due to the introgression of L. pimpinellifolium DNA at the Pto locus (Pitblado and MacNeill, 1983;Martin et al, 1993a). DNAs from susceptible mutants analyzed with RFLP markers linked to the Pfo locus were found to carry fragments specific for 76R (data not shown), indicating that these lines were true mutants.…”

Section: Lsolation Of Tomato Mutants Susceptiblementioning

confidence: 99%

“…To quantify the degree to which the mutants were susceptible, the growth of Tl(avrPto) was monitored in the mutant plants, as described in Figure 1. Bacterial growth in the mutant plants was compared to growth of the same strain in the wild-type resistant line 76R and its near-isogenic-susceptible relative Rio Grande76s (76S), which differs from 76R in that it lacks the introgressed region containing the Pto locus (Pitblado and MacNeill, 1983 …”

Section: R S Tomatomentioning

confidence: 99%

“…s. tomato strains that express the avirulence gene avrffo (Ronald et al, 1992) is governed by the incompletely dominant Pto resistance locus on chromosome 5 (Pitblado et al, 1984;Carland and Staskawicz, 1993;Martin et al, 1993a). Pto was introgressed into Lycopefsicon esculentum cultivars from the sexually compatible resistant species L. pimpinellifolium (Pitblado and MacNeill, 1983). Morphological and molecular markers tightly linked to Pto have been identified (Carland and Staskawicz, 1993;Martin et al, 1993a), and molecular cloning of the Pto gene has recently been accomplished using a positional cloning strategy (Martin et al, 1993b).…”

Section: Introductionmentioning

confidence: 99%

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Tomato mutants altered in bacterial disease resistance provide evidence for a new locus controlling pathogen recognition.

et al. 1994

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We have employed a genetic approach to study the resistance of tomato to the phytopathogenic bacterium Pseudomonas syringae pv tomato. Resistance to I? s. tomato depends upon expression of the Pto locus in tomato, which encodes a protein with similarity to serinehhreonine protein kinases and recsgnizes pathogen strains expressing the avirulence gene avrPto. Eleven tomato mutants were isolated with altered resistance to I? s. tomato strains expressing avrPto. We identified mutations both in the Pto resistance locus and in a new locus designated Prf (for Pseudomonas resistance and fenthion sensitivity). The genetic approach allowed us to dissect the roles of these loci in signal transduction in response to pathogen attack. Lines carrying mutations in the Pto locus vary 200-fold in the degree to which they are susceptible to I? s. tomato strains expressing avrPto. The pto mutants retain sensitivity to the organophosphate insecticide fenthion; this trait segregates with Pto in genetic crosses. This result suggested that contrary to previous hypotheses, the Pto locus controls pathogen recognition but not fenthion sensitivity. Interestingly, mutations in the prf locus result in both complete susceptibility to I? s . tomato and insensitivity to fenthion, suggesting that Prf plays a role in tomato signaling in response to both pathogen elicitors and fenthion. Because pto and prf mutations do not alter recognition of Xanthomonas campestris strains expressing avrBsR an avirulence gene recognized by all tested tomato cultivars, Prf does not play a general role in disease resistance but possibly functionsspecifically in resistance against I? s. tomato.Genetic analysis of F2 populations from crosses of pto and prf homozygotes indicated that the Pto and Prf loci are tightly lin ked .

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Section: Lsolation Of Tomato Mutants Susceptiblementioning

confidence: 99%

Section: R S Tomatomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tomato mutants altered in bacterial disease resistance provide evidence for a new locus controlling pathogen recognition.

et al. 1994

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“…P. syringae pv. tomato is controlled by a single resistance locus, Pto (29). Pto was derived from the sexually compatible wild species Lycopersicon pimpinellifolium (28) and maps to the short arm of chromosome 5 at map position 30 (30).…”

mentioning

confidence: 99%

The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance gene

et al. 1992

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“…Therefore breeding of tomatoes for genetic resistance against bacterial speck seems to be very important and promising perspective. It was found that resistance of Lycopersicon esculentum 'Ontario 7710' to bacterial speck is determined by a single dominant gene (Pitblado and MacNeill, 1983). Also, the evidence that this resistance is governed by semi-dominant gene was found (Kozik, 2002).…”

Section: Introductionmentioning

confidence: 87%

Genetic and Morpho-Agronomic Evaluation of New Tomato Breeding Lines Resistant to Bacterial Speck (Pseudomonas Syringae pv. Tomato)

Kozik¹,

Nowakowska²

2010

JAS

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A breeding program was initiated at the Research Institute of Vegetable Crops, Skierniewice, Poland to select new
tomato lines resistant to bacterial speck (Psedomonas syringae pv. tomato (PST) and suitable for field growing. Two
tomato cultivars with good agronomic characteristics Luban and Rumba were crossed with resistant to PST ‘Ontario
7710’. Then backcross and pedigree breeding procedures were employed to obtain BC5, BC4S1, BC3S2, BC2S3, BC1S4
populations. Screening test among those hybrid populations was performed in a greenhouse and exhibited a large
amount of variation in response to PST infection. All plants within BC1S4 progeny of the ‘Luban’ x ‘Ontario 7710’
cross and two BC1S4, BC3S2 progenies of the ‘Rumba’ x ‘Ontario 7710’ cross had no symptoms of bacterial speck and
could be assigned to the homozygous category. The remaining progenies were segregating for resistance and
susceptibility and gave distribution of plants over all severity classes. That confirms heterozygosity for resistant gene
Pto of their latter resistant single plant selections before backcrossing or/and selfing. The BC5 populations had
significantly higher disease severity rates (DSI = 2.4) than all other selfed populations (DSI = 1.2 – 1.6). After selection
resistant genotypes of the populations were transplanted to the field for morphological traits evaluation. A noticeable
progress in breeding regarding most of the plant and fruit characteristics of the recurrent parents was approached in all
populations of the ‘Luban’ x ‘Ontario 7710’ cross. Although all progenies of the ‘Rumba’ x ‘Ontario 7710’ differed
from their recurrent parent as to one feature at least, however the differences showed improved shifting toward the
characteristics of susceptible parent

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Genetic basis of resistance toPseudomonas syringaepv.tomatoin field tomatoes

Cited by 42 publications

References 9 publications

Tomato mutants altered in bacterial disease resistance provide evidence for a new locus controlling pathogen recognition.

Tomato mutants altered in bacterial disease resistance provide evidence for a new locus controlling pathogen recognition.

The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance gene

Genetic and Morpho-Agronomic Evaluation of New Tomato Breeding Lines Resistant to Bacterial Speck (Pseudomonas Syringae pv. Tomato)

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