Genetic characterisation and breed assignment in Austrian sheep breeds using microsatellite marker information

Baumung, Roswitha; Čubrić-Ćurik, Vlatka; Schwend, Katharina; Achmann, Roland; Sölkner, Johann

doi:10.1111/j.1439-0388.2006.00583.x

Cited by 67 publications

(78 citation statements)

References 21 publications

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“…Lack of differentiation between the Sicilian breeds could be due to geographic proximity, similarities in environment and breeding practices, but most likely due to past gene flow among them. These estimates of genetic differentiation are comparable to those reported by other authors for indigenous Ethiopian (F st = 0.046, Gizaw et al, 2007), Portuguese (F st = 0.049, Santos-Silva et al, 2008), and Alpine (F st = 0.057, Dalvit et al, 2008) sheep breeds and/or populations, but lower than those reported by Baumung et al (2006) in Austrian sheep breeds and Arora et al (2011) in Indian sheep breeds (0.080 and 0.111, respectively). Moreover, Lawson Handley et al (2007) in a study on European sheep breeds, including the COM and the SAR, reported that the Southern breeds are characterized by higher within-breed diversity, lower genetic differentiation, and higher level of heterozygosity than Northern breeds.…”

Section: Discussionsupporting

confidence: 76%

Genetic diversity and population structure of Sicilian sheep breeds using microsatellite markers

Tolone

Mastrangelo

Rosa

et al. 2012

Small Ruminant Research

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a b s t r a c tGenetic diversity studies in domestic animals aim at evaluating genetic variation within and across breeds mainly for conservation purposes. In Sicily, dairy sheep production represents an important resource for hilly and mountain areas economy. Their milk is used for the production of traditional raw milk cheeses, sometimes protected designation of origin (PDO) cheeses. In some cases, the quality of these products is linked to a specific breed, i.e. mono-breed labelled cheeses and it is therefore important to be able to distinguish the milk of a breed from that of others, in order to guarantee both the consumer and the breed itself. In order to investigate the genetic structure and to perform an assignment test, a total of 331 individuals (Barbaresca, BAR n = 57, Comisana, COM n = 65, Pinzirita, PIN n = 75, Sarda, SAR n = 64, and Valle del Belice, VDB n = 70) were analysed using a panel of 20 microsatellite markers. A total of 259 alleles were observed with average polymorphic information content equal to 0.76, showing that the microsatellites panel used was highly informative. Estimates of observed heterozygosity ranged from 0.65 in the BAR breed to 0.75 in the COM breed. The low value of genetic differentiation among breeds (F st = 0.049) may indicate that these breeds are little differentiated probably due to common history and breeding practices. The low F is and F it values indicated low level of inbreeding within and among breeds. The unrooted neighbor-joining dendrogram obtained from the Reynold's genetic distances, and factorial correspondence analysis revealed a separation between BAR and the other sheep breeds. Recent migration rates were estimated, showing that four out of the five breeds have not received a significant proportion of migrants. Only for the PIN breed a recent introgression rate from the VDB breed (7.2%) was observed. The Bayesian assignment test showed that BAR and SAR breeds had a more definite genetic structure (proportion of assignment of 92% and 86.6%, respectively), whereas the lowest assignment value was found in the PIN breed (67.1%). Our results indicated high genetic variability, low inbreeding and low genetic differentiation, except for BAR breed, and were in accordance with geographical location, history, and breeding practices. The low robustness of the assignment test makes it unfeasible for traceability purposes, due to the high level of admixture, in particular for COM, PIN and VDB.

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Section: Discussionsupporting

confidence: 76%

Genetic diversity and population structure of Sicilian sheep breeds using microsatellite markers

Tolone

Mastrangelo

Rosa

et al. 2012

Small Ruminant Research

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“…To avoid bias because of unequal sample sizes, particularly for the Milchschaf breed, the bootstrapping method recommended by Simianer (2002) and Baumung et al (2006) was applied using 1000 samples with exactly 50 individuals per breed.…”

Section: Discussionmentioning

confidence: 99%

Genetic relationships between Spanish Assaf (Assaf.E) and Spanish native dairy sheep breeds

Legaz¹,

Álvarez

Royo

et al. 2008

Small Ruminant Research

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a b s t r a c tAt present, the Assaf is the main dairy sheep in Spain. The Spanish Assaf (Assaf.E) was formed by male-mediated absorption of native Spanish sheep. Here we assess the genetic relationships among the Assaf.E and major native Spanish dairy breeds using microsatellites to contribute to the knowledge of the formation and within-population genetic variability of the breed. Blood samples from 44 unrelated Assaf.E individuals from 23 different Assaf.E flocks spread throughout 6 different Spanish provinces were obtained and genotyped using 14 microsatellites. Up to 312 additional samples belonging to the Awassi and Milchschaf sheep breeds and to six native Spanish dairy sheep breeds (Castellana, Churra, Latxa, Manchega, and Rubia de El Molar) as well as samples from Merino individuals to be used as the outgroup were also analysed observed (H o ) and expected (H e ) heterozygosity, rarefacted number of alleles per locus and distances based on molecular coancestry information were computed. Probabilities of assignment of the Assaf.E individuals to native Spanish dairy sheep breeds and cryptic genetic structure in the whole dataset were also assessed. It can be concluded that the Assaf.E breed has low genetic variability and high genetic distance with respect native Spanish dairy sheep breeds. From our results, the formation of the Assaf.E breed basically occurred via the absorption of individuals belonging to the Entrefino type, particularly to the Castellana and Manchega populations. Furthermore, Churra individuals may have participated in the formation of the Assaf.E breed at an early moment of the introduction of the breed into Spain.

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“…have used 25 microsatellites in 20 native and 12 imported North European sheep breeds in order to evaluate the importance of each breed for gene diversity. Baumung et al, (2006) have used 25 microsatellite markers for genetic characterization and breed assignment in 11 Austrian sheep breeds. Peter et al, (2007) have examined in a comprehensive study the genetic diversity of 57 European and Middle Eastern sheep breeds, using 31 microsatellites markers.…”

Section: Analysis Of Genetic Variation In Animalsmentioning

confidence: 99%

“…Microsatellites are used, in livestock species for estimating genetic variation within and among breeds (Buchanan et al, 1994;Cronin et al, 2009;Diez-Tascon et al, 2000a;Dowling et al, 2008;Schmid et al, 1999;Saitbekova et al, 1999), for admixture studies (Alvarez et al, 2004;Freeman et al, 2004Freeman et al, , 2006MacHugh et al, 1997;Vicente et al, 2008) and for assigning individuals to breeds (Baumung et al, 2006;Cornuet et al, 1999;Maudet et al, 2002;Meadows et al, 2006;Troy et al, 2001). …”

Section: Analysis Of Genetic Variation In Animalsmentioning

confidence: 99%

Genetic Characterization of Albanian Sheep Breeds by Microsatellite Markers

Hoda¹,

Marsan²

2012

Analysis of Genetic Variation in Animals

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Genetic characterisation and breed assignment in Austrian sheep breeds using microsatellite marker information

Cited by 67 publications

References 21 publications

Genetic diversity and population structure of Sicilian sheep breeds using microsatellite markers

Genetic diversity and population structure of Sicilian sheep breeds using microsatellite markers

Genetic relationships between Spanish Assaf (Assaf.E) and Spanish native dairy sheep breeds

Genetic Characterization of Albanian Sheep Breeds by Microsatellite Markers

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