Genetic characteristics and geographic segregation of Giardia duodenalis in dairy cattle from Guangdong Province, southern China

Cui, Zhaohui; Wang, Luyang; Cao, Letian; Sun, Mingfei; Liang, Nan; Wang, Haiyan; Chang, Yankai; Lin, Xuhui; Yu, Linzeng; Wang, Rongjun; Ning, Changshen; Zhang, Longxian

doi:10.1016/j.meegid.2018.09.019

Cited by 22 publications

(20 citation statements)

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“…Other studies conducted in Shanghai (37.0% or 303/818; [11]), Henan (36.2% or 108/298; [8]), and northeastern China (32.1% or 61/190 to 47.7% or 72/151; [18, 54]) reported higher detection rates of Cryptosporidium spp. In contrast, the 74.2% (288/388) detection rate of G. duodenalis obtained in this study is the highest among detection rates reported in pre-weaned dairy calves in China to date with previous studies reporting detection rates of 2.7% (11/404) to 60.1% (492/818) [12–14, 16, 17, 24, 25, 27, 29, 32, 44, 47, 55, 56]. The detection rate of E. bieneusi in this study was found to be 15.7% (61/388), which is within the range of reported detection rates of 2.0% (3/148) to 37.6% (35/93) in China [8, 17, 27, 36, 39–41, 46, 57].…”

Section: Discussioncontrasting

confidence: 69%

“…in dairy cattle in the province, which identified C. bovis , C. ryanae and C. andersoni in pre-weaned dairy calves. There has also only been one study on G. duodenalis in the province, which reported a positive rate of 8.4% and identified the presence of assemblage E [47]. No studies have been conducted on E. bieneusi infections in pre-weaned dairy calves in this province.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in pre-weaned dairy calves in Guangdong, China

et al. 2019

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BackgroundCryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi are common enteric pathogens in humans and animals. Data on the transmission of these pathogens are scarce from Guangdong, China, which has a subtropical monsoon climate and is the epicenter for many emerging infectious diseases. This study was conducted to better understand the prevalence and identity of the three pathogens in pre-weaned dairy calves in Guangdong.MethodsThe occurrence and genetic identity of three pathogens were analyzed by polymerase chain reaction. PCR-positive products were sequenced to determine the species and genotypes. A Chi-square test was used to compare the prevalence of pathogens among sampling dates, age groups, or clinical signs.ResultsThe detection rates of Cryptosporidium spp., G. duodenalis and E. bieneusi were 24.0% (93/388), 74.2% (288/388) and 15.7% (61/388), respectively. Three Cryptosporidium species were detected, including C. bovis (n = 73), C. parvum (n = 12) and C. ryanae (n = 7); one animal had concurrence of C. bovis and C. parvum. C. parvum was the dominant species during the first two weeks of life, whereas C. bovis and C. ryanae were mostly seen at 3–9 weeks of age. Sequence analysis identified the C. parvum as subtype IIdA19G1. Assemblage E (n = 282), assemblage A (n = 1), and concurrence of A and E (n = 5) were identified among G. duodenalis-positive animals using multilocus genotyping (MLG). Altogether, 15, 10 and 17 subtypes of assemblage E were observed at the bg, gdh and tpi loci, respectively, forming 49 assemblage E MLGs. The highest detection rate of G. duodenalis was found in winter. Sequence analysis identified genotypes J (n = 57), D (n = 3) and one concurrence of J and D among E. bieneusi-positive animals. The detection rate of E. bieneusi was significantly higher in spring (38.0%; 41/108) than in summer (7.2%; 8/111) and winter (7.1%; 12/169).ConclusionsThese results indicate a common occurrence of C. parvum subtype IIdA19G1, G. duodenalis assemblage E, and E. bieneusi genotype J in pre-weaned dairy calves in Guangdong. More studies are needed to understand the unique genetic characteristics and zoonotic potential of the three enteric pathogens in the province.

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Section: Discussioncontrasting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in pre-weaned dairy calves in Guangdong, China

et al. 2019

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“…duodenalis , which was similar to the occurrence in Hubei (37.8%, 28/74) [16], but much higher than the majority of provinces in China. These provinces included Jilin (4.4%, 5/114) [14], Liaoning (3.1%, 3/98) [14], Heilongjiang (12.5%, 3/24) [14], Shaanxi (17.54%, 30/171) [20], Xinjiang (16.6%, 46/277) [15] and Guangdong (1.1%, 5/533) [17]. Compared with studies in other countries, the overall infection rate in the present study was higher than in Maryland, USA (32.1%, 125/390) [11]; Malaysia (8.3%, 10/120) [8]; India (12.5%, 9/72) [10] and New Zealand (2%, 2/100) [9], but lower than that in another study in the USA (52%, 237/456) [12].…”

Section: Resultsmentioning

confidence: 99%

“…MLG E69, MLG E78, MLG E83, MLG E87 and MLG E98 were singletons. To better understand the diversity between assemblage E MLGs in Sichuan and in the other provinces of China (Henan [30], Gansu [31], Ningxia [31], Shaanxi [20], Xinjiang [15], Shanghai [28] and Guangdong [17, 27]), a phylogenetic evolutionary tree was constructed. The phylogenetic tree (S2 Fig) showed that MLG E3 has also been found in Gansu [31], Xinjiang [15], Shanghai [28] and Guangdong [27], which indicates the wide distribution of this MLG subtype in China.…”

Section: Resultsmentioning

confidence: 99%

“…duodenalis in dairy calves, and the occurrence has been found to be significantly different between pre- and post-weaned stages [8–13]. In China, studies have also revealed different infection rates in pre- and post-weaned dairy calves, e.g., in Liaoning [14], Xinjiang [15], Hubei [16] and Guangdong [17] provinces. In our previous study, we conducted a preliminary study on G .…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China

et al. 2019

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Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G. duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3–12 months old) from 10 farms, including 4 intensive feeding farms and 6 free-ranging farms. The overall infection rate of G. duodenalis was 41.2% (126/306) based on the PCR results at any of the three genetic loci: beta-giardin (bg), triose-phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Giardia duodenalis assemblages E (n = 115, 91.3%), A (n = 3, 2.4%), and A mixed with E (n = 8, 6.3%) were identified among the 126 positive specimens. Multilocus sequence typing of G. duodenalis revealed 34 assemblage E multilocus genotypes (MLGs), 1 assemblage A MLG and 7 mixed assemblage (A and E) MLGs. The eBURST data showed a high degree of genetic diversity within assemblage E MLGs. The phylogenetic tree revealed that MLG E3 was the primary MLG subtype in Sichuan province and also the most widely distributed in China.

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Infection patterns, clinical significance, and genetic characteristics of Enterocytozoon bieneusi and Giardia duodenalis in dairy cattle in Jiangsu, China

Wang

Jiang

et al. 2019

Parasitol Res

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Genetic characteristics and geographic segregation of Giardia duodenalis in dairy cattle from Guangdong Province, southern China

Cited by 22 publications

References 37 publications

Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in pre-weaned dairy calves in Guangdong, China

Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in pre-weaned dairy calves in Guangdong, China

Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China

Infection patterns, clinical significance, and genetic characteristics of Enterocytozoon bieneusi and Giardia duodenalis in dairy cattle in Jiangsu, China

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