Genetic characteristics of mumps viruses isolated in Korea from 2007 to 2012

Abstract

Mumps is a vaccine-preventable viral disease. Despite vaccine coverage of >95%, the incidence of mumps has increased in Korea since 2007. This study aimed to genetically characterize mumps virus (MuV) strains that circulated in Korea between 2007 and 2012 to determine the factors underlying mumps outbreaks. MuV was isolated from 175 clinical specimens between 2007 and 2012 in Korea. Upon analysis of the SH gene in Korean mumps virus isolates, three different genotypes were identified: I, H, and F. The MuV genotypes I and H co-circulated in Korea, and eight isolates of Korean genotype F were found within the same time period in 2008. An analysis of HN amino-acid sequence data showed that Korean isolates had no changes in their glycosylation sites. At putative neutralizing epitope sites, the Jeryl-Lynn strain showed 4-5 different amino acid sequences from those observed in Korean isolates. Korean isolates of genotypes I and H shared distinctive point mutations on putative neutralizing epitope positions in each genotype. This report describes the genetic characteristics of MuV strains circulating in Korea and provides information on endemic mumps infections. This information may be important to help prevent mumps and control outbreaks of mumps in Korea. J. Med. Virol. 88:1479-1486, 2016. © 2016 Wiley Periodicals, Inc.

Viral etiology of sporadic cases of parotitis among children in Korea during 2013‐2014

“…All of these sequences were identified as genotype I (data not shown). Based on our previous study, the MuV genotypes I and H were co‐circulated in Korea in 2007‐2012, and the mumps mainly occurred in middle school and high school students . The incidence of mumps is generally highest during the school year in Korea (from March to July and from September to December), but the sporadic cases of mumps in this study did not match this general pattern.…”

“…The sequences of the primers and probes were as follows: forward primer, 5′‐AGT GTC GAT CTC ATY AGG TAC TA‐3′; reverse primer, 5′‐TGG ATY GCC GGC ATA GTG C‐3′; probe, 5′‐(FAM) ACG GCA GGG TCA CGA GA (MGBNFQ)‐3′. The cycling conditions were 50°C for 30 min; 95°C for 10 min; 40 cycles of 95°C for 15 s, followed by 60°C for 60 s. MuV genotyping was performed using the amplified SH gene, and the DNA was sequenced using Genetic Analyzer (Applied Biosystems, Foster City, CA). Positive samples determined by real‐time RT‐PCR were confirmed by the isolation of mumps virus in Vero cells.…”

Parotiditis en Chile: caracterización clínica y molecular de dos casos en una población altamente inmunizada

“…Durante los últimos años, se han descrito brotes de parotiditis prácticamente en todo el mundo [11][12][13][14][15] . Estos brotes, frecuentes en comunidades cerradas, han demostrado la "re-emergencia" de la enfermedad y la transmisión efectiva en una población con alta cobertura de la vacuna 8,11 .…”

“…La tasa de incidencia de parotiditis disminuyó de 198 casos x 100.000 habitantes, antes de la vacunación, a 9 casos x 100.000 habitantes, en el año 2007 9,10 . Sin embargo, a pesar de la efectividad de la vacuna, se continúan reportando brotes en todo el mundo [11][12][13][14][15] . Además, la vacunación ha generado un cambio epidemiológico, trasladándose la edad de presentación clínica de la parotiditis desde niños y adolescentes a adultos jóvenes 16 .…”