Genetic characterization and phylogenetic position of Echinococcus felidis   (Cestoda: Taeniidae) from the African lion

Hüttner, M.; Nakao, Minoru; Wassermann, Marion; Siefert, L.; Boomker, Jacob Diederik Frederik; Dinkel, Anke; Sako, Yasuhito; Mackenstedt, Ute; Römig, Thomas; Itō, Akira

doi:10.1016/j.ijpara.2007.10.013

Cited by 250 publications

(222 citation statements)

References 38 publications

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“…shiquicus, the nuclear DNA phylogenies of Knapp et al (2011) are in agreement with the mtDNA phylogenies of Nakao et al (2007Nakao et al ( , 2013b and Hüttner et al (2008), while the phylogeny of Saarma et al (2009) suggests a different topology. With respect to E. canadensis, all phylogenies indicate a monophyletic origin, except for that based on exon sequences of nuclear DNA genes by Knapp et al (2011), which suggests that the G6 genotype is more closely related to E. ortleppi than to the other genotypes of E. canadensis.…”

Section: Phylogenetic Patternsupporting

confidence: 61%

“…They occur in the same localities in southern and eastern Africa, with fixed differences in both mtDNA and nuclear DNA sequences, as well as consistent differences in adult morphology and definitive host occurrence; usually canids for E. granulosus and lion (Panthera leo) or spotted hyena (Crocuta crocuta) for E. felidis (Hüttner et al, 2008;Kagendo et al, 2014).…”

Section: Echinococcus Granulosus and E Felidismentioning

confidence: 87%

“…Le et al, 2002;McManus et al, 2002;Obwaller et al, 2004;Lavikainen et al, 2003Lavikainen et al, , 2006Thompson et al, 2006;Nakao et al, 2007Nakao et al, , 2013bHüttner et al, 2008;Moks et al, 2008) and nuclear DNA sequences (e.g. Lavikainen et al, 2003;Bart et al, 2006;Saarma et al, 2009;Knapp et al, 2011).…”

Section: Phylogenetic Patternmentioning

confidence: 99%

“…Nakao et al (2007) subsequently proposed that the remaining strains (G6, G7, G8 and G10) be afforded species status as E. canadensis. In addition to these taxonomic changes, Xiao et al (2005) described a new species (E. shiquicus), closely related to E. multilocularis, from Tibet, and Hüttner et al (2008) resurrected the species E. felidis, originally described by Ortlepp (1934Ortlepp ( , 1937) from South Africa. As a consequence of these revisions and new descriptions, nine valid species are currently recognised in the genus Echinococcus (Table 1).…”

mentioning

confidence: 99%

“…In many cases, where there have been no obvious differences in morphology between putative species, a genetic yardstick has been used; species status has been recommended if taxa are as genetically different from each other as the difference between well-established species in the same or related genera (e.g. Thompson and McManus, 2002;Nakao et al, 2007;Hüttner et al, 2008). While a genetic yardstick approach may sometimes provide a useful guide to species status, it is essentially an appeal to authority, or at least to past practice.…”

mentioning

confidence: 99%

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Phylogenetic Pattern, Evolutionary Processes and Species Delimitation in the Genus Echinococcus

Lymbery

2017

Advances in Parasitology

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Section: Phylogenetic Patternsupporting

confidence: 61%

Section: Echinococcus Granulosus and E Felidismentioning

confidence: 87%

Section: Phylogenetic Patternmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Phylogenetic Pattern, Evolutionary Processes and Species Delimitation in the Genus Echinococcus

Lymbery

2017

Advances in Parasitology

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Cystic Echinococcosis

Brunetti

2009

Tropical Diseases in Travelers

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Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning‐phylogenetic approach

et al. 2009

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In the present study, we have extended earlier taxonomic, biochemical and experimental investigations to characterize Echinococcus granulosus from various hosts in Iran utilizing DNA regions (designated pcox1 and pnad1) within the cytochrome c oxidase subunit 1 and NADH dehydrogenase 1 mitochondrial genes, respectively. An emphasis was placed on the characterization of E. granulosus isolates (cyst material) from humans, sheep, goats, cattle and camels, and on assessing their genetic relationships. PCR-based SSCP analysis of pcox1 and pnad1 amplicons derived from individual isolates (n=148) of E. granulosus revealed five (pc1-pc5) and nine (pn1-pn9) electrophoretic profiles, respectively. Sequencing of pcox1 and pnad1 amplicons representing unique SSCP profiles demonstrated that each profile was linked unequivocally to a particular sequence and that single point mutations were readily detectable by SSCP. Phylogenetic analyses of pcox1 and/or pnad1 nucleotide sequence data were conducted using Bayesian inference and maximum likelihood tree-building methods. Following the phylogenetic analyses of concatenated pcox1+pnad1 sequence data, including representatives of all presently recognized Echinococcus species/genotypes as well as Taenia saginata (as the outgroup), the majority of cyst isolates (142 of 148; 95.9%) from humans, ruminants (sheep, goats and cattle) and camels were assigned to the G1-G3 complex of E. granulosus (or E. granulosus sensu stricto), whereas some E. granulosus cysts (6 of 19; 31.6%) from camels were assigned to the G6-G10 complex (or E. canadensis). The present study reinforces the advantages of the mutation scanning-sequencing-phylogenetic approach to explore variation in multiple mitochondrial loci within and among Echinococcus populations, which provides a platform for future, detailed studies of the molecular epidemiology of E. granulosus in Iran and other countries. (Note: The sequences determined in the present study have been deposited in the GenBank database under accession numbers: FJ796203-FJ796207 (pcox1) and FJ796208-FJ796216 (pnad1)).

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Genetic characterization and phylogenetic position of Echinococcus felidis (Cestoda: Taeniidae) from the African lion

Cited by 250 publications

References 38 publications

Phylogenetic Pattern, Evolutionary Processes and Species Delimitation in the Genus Echinococcus

Phylogenetic Pattern, Evolutionary Processes and Species Delimitation in the Genus Echinococcus

Cystic Echinococcosis

Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning‐phylogenetic approach

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