Genetic characterization and recombination analysis of atypical porcine pestivirus

Guo, Zhenhua; Wang, Leyi; Qiao, Songlin; Deng, Ruiguang; Zhang, Gaiping

doi:10.1016/j.meegid.2020.104259

Cited by 17 publications

(28 citation statements)

References 25 publications

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“…Sequence identity between OVPV and CSFV was as high as 89.9% in the 5 UTR region. These results revealed that the Italian OVPV is more closely related to CSFV than BDV, BVDV, or any other of the existing or recently-discovered pestiviruses, such as Aydin, LINDA, or APPV [12][13][14].…”

Section: Introductionmentioning

confidence: 81%

Decrypting the Origin and Pathogenesis in Pregnant Ewes of a New Ovine Pestivirus Closely Related to Classical Swine Fever Virus

Wang¹,

Sozzi

Bohórquez³

et al. 2020

Viruses

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This study shows the origin and the pathogenic role of a novel ovine pestivirus (OVPV) isolated in 2017 in Italy, as a pathogenic agent causing severe abortions after infection in pregnant ewes and high capacity for virus trans-placental transmission as well as the birth of lambs suffering OVPV-persistent infection. The OVPV infection induced early antibody response detected by the specific ELISA against classical swine fever virus (CSFV), another important virus affecting swine. The neutralizing antibody response were similar against CSFV strains from genotype 2 and the OVPV. These viruses showed high identity in the B/C domain of the E2-glycoprotein. Close molecular diagnostics cross-reactivity between CSFV and OVPV was found and a new OVPV molecular assay was developed. The phylodynamic analysis showed that CSFV seems to have emerged as the result of an inter-species jump of Tunisian sheep virus (TSV) from sheep to pigs. The OVPV and the CSFV share the TSV as a common ancestor, emerging around 300 years ago. This suggests that the differentiation of TSV into two dangerous new viruses for animal health (CSFV and OVPV) was likely favored by human intervention for the close housing of multiple species for intensive livestock production.

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Section: Introductionmentioning

confidence: 81%

Decrypting the Origin and Pathogenesis in Pregnant Ewes of a New Ovine Pestivirus Closely Related to Classical Swine Fever Virus

Wang¹,

Sozzi

Bohórquez³

et al. 2020

Viruses

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“…According to phylogenetic dendrograms and pairwise sequence comparisons, APPVs are subdivided into three genotypes (Choe et al, 2020;Folgueiras-Gonzalez et al, 2020;Guo et al, 2020;Xie et al, 2019;Yan et al, 2019). Our analyses showed that Anna/2020 belonged to genotype 3; however, Anna/2020 formed an independent branch, and was distantly related to the Chinese genotype 3 strains.…”

Section: Discussionmentioning

confidence: 64%

“…While there is 94.0% to 99.7% sequence identity among the Chinese APPVs in genotype 3, Anna/2020 showed 87.0% to 89.3% identity to these Chinese APPVs in genotype 3 (Table 1). Recombination events are an important mechanism by which viruses acquire genetic diversity, and they have been identified in APPVs (Guo et al, 2020;Xiao et al, 2016). Thus, to find crossover sites between Anna/2020 and other APPVs, similarity plot analysis was performed using SimPlot software.…”

Section: Analysis Of the Whole Genome Sequence Of The Japanese Appvmentioning

confidence: 99%

Detection and genetic analysis of a novel atypical porcine pestivirus from piglets with congenital tremor in Japan

Kasahara-Kamiie

Kagawa²,

Mai

et al. 2021

Preprint

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Atypical porcine pestivirus (APPV), which has been confirmed to be associated with congenital tremor (CT) in pigs, is a newly discovered porcine virus that has been found in the Americas, Europe, and Asia; however, no report of APPV in Japan has been published. We identified an APPV in the central nervous system of Japanese piglets with CT, and firstly determined and analyzed the complete genome sequence. Phylogenetic analysis using the complete genome nucleotide sequence of the Japanese APPV, named Anna/2020, and those of APPVs from the NCBI database showed that APPVs were divided into three genotypes (genotypes 1 to 3), and that Anna/2020 clustered with the genotype 3 APPV strains, but distantly branched from these strains. Pairwise complete coding region nucleotide sequence comparisons revealed that there was 94.0% to 99.7% sequence identity among the genotype 3 strains, while Anna/2020 showed 87.0% to 89.3% identity to those genotype 3 strains, suggesting that Anna/2020 represents a novel APPV lineage within genotype 3. Retrospective examinations using RT-PCR revealed one genotype 1 and two novel genotype 3 APPVs from pigs without CT, and that novel genotype 3 APPVs have been prevalent in Japan since at least 2007.

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“…In the three ML trees (NS3, E2, and Npro) constructed for this study, Clade I contained four subclades; however, the APPV strains contained within these subclades were not clearly distinguished by the three ML trees. A previous study suggests that recombination events occur between Clades (Clades II and III) or within a Clade (Clade I) [ 25 ]. Therefore, further research is needed to determine whether the difference in subclade of South Korean APPV strains in three ML trees are because of the recombination events within Clade I.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Genetic Diversity of Atypical Porcine Pestivirus (APPV) Detected in South Korean Wild Boars

Choe

Park

et al. 2020

Viruses

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Atypical porcine pestivirus (APPV), currently classified as pestivirus K, causes congenital tremor (CT) type A-II in piglets. Eighteen APPV strains were identified from 2297 South Korean wild boars captured in 2019. Phylogenetic analysis of the structural protein E2 and nonstructural proteins NS3 and Npro classified the APPV viruses, including reference strains, into Clades I, II and III. Clade I was divided into four subclades; however, the strains belonging to the four subclades differed slightly, depending on the tree analysis, the NS3, E2, and Npro genes. The maximum-likelihood method was assigned to South Korean wild boar APPV strains to various subclades within the three trees: subclades I.1 and I.2 in the E2 tree, subclade I.1 in the Npro tree, and subclades I.1 and I.4 in the NS3 ML tree. In conclusion, APPV among South Korean wild boars belonging to Clade I may be circulating at a higher level than among the South Korean domestic pig populations.

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Genetic characterization and recombination analysis of atypical porcine pestivirus

Cited by 17 publications

References 25 publications

Decrypting the Origin and Pathogenesis in Pregnant Ewes of a New Ovine Pestivirus Closely Related to Classical Swine Fever Virus

Decrypting the Origin and Pathogenesis in Pregnant Ewes of a New Ovine Pestivirus Closely Related to Classical Swine Fever Virus

Detection and genetic analysis of a novel atypical porcine pestivirus from piglets with congenital tremor in Japan

Prevalence and Genetic Diversity of Atypical Porcine Pestivirus (APPV) Detected in South Korean Wild Boars

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