Genetic characterization of green bean (Phaseolus vulgaris) genotypes from eastern Turkey

Abstract

ABSTRACT. Green bean genotypes collected from eastern Turkey were characterized using simple sequence repeat (SSR) markers and morphological traits. Among 12 SSR markers, 10 produced successful amplifications and revealed DNA polymorphisms that were subsequently used to assess genetic relatedness of the genotypes. Based on the number of alleles generated and the probability of identity values, the most informative SSR loci were PVGLND5, PVMEIG, PVag001, and PV-ag004. Probably, due to the inbreeding nature of beans, the heterozygosity observed within genotypes was low at most of the SSR loci. The UPGMA dendrogram constructed based on the SSR data yielded two major clusters. The overall genetic distance was around 98%, among the genotypes. This information can be used to help select Turkish green bean lines.

Preserving Biodiversity in Marginal Rural Areas: Assessment of Morphological and Genetic Variability of a Sicilian Common Bean Germplasm Collection

“…The discriminating power for each locus was comparable or higher than that reported for the same SSR locus (GATS91, BM210, BM160, PVBR25, BM172, PVBR163) in previous Italian collection studies [47][48][49]. Furthermore, mean He across eight SSR loci was higher (0.70) in our collection compared to those observed in other studies carried out on Italian [9,35,[47][48][49] as well as European collections [37,[60][61][62]. The overall loci probability of identity (PI) and PIC values obtained in our study prove the ability of this SSR panel to distinguish among common bean landraces of Nebrodi Mountains.…”

Molecular Diversity of Kenyan Lablab Bean (<i>Lablab purpureus</i> (L.) Sweet) Accessions Using Amplified Fragment Length Polymorphism Markers

“…In most cultivated plant species, higher mean heterozygosity (He) have been reported. Some of the mean heterozygosity reported for plants are 0.30 in soyabean [33]; 0.32 in common beans using AFLP markers [34]; 0.361 in green beans using SSR markers [35]; 0.444 for mung bean using SSR markers [36], 0.361 in tea using AFLP markers [37], 0.313 in rice using SSR markers [38] and 0.37 in wheat with SSR markers [39].…”

Characterization of genetic diversity in Turkish common bean gene pool using phenotypic and whole-genome DArTseq-generated silicoDArT marker information

“…Northeast Anatolian region. Another study reported genetic diversity of 30 genotypes from two districts of Van province i.e., Ercis and Gevas [ 30 ], while Nemli et al [ 31 ] used iPBS markers for the characterization of the 67 Turkish common bean landraces, and, in their recent work, Nemli et al [ 32 ] used SNP markers for the determination of diversity in the Turkish common bean.…”