Salt-tolerant Gevas Sirik 57 (GS57) genotypes and salt-sensitive 4F-89 French variety, previously determined in our preliminary study, were used in the study. The genotype and the variety exposed to 100mM NaCl application developed different mechanisms to be protected against toxic effects of Na + ion. Salt-sensitive 4F-89 French variety let Na + accumulate in all organs. On the contrary, salt-tolerant GS57 did not avoid salt and acted selectively among ions; the majority of toxic ion Na + accumulated in old leaves and shoots and the plants did not transport them into young leaves. K + accumulation was high in organs in which Na + concentrations were low, and vice versa; Na + content was low in young and high in old leaves of GS57, but K + content was opposite. Ca 2+ content in young leaves of GS57 and 4F-89 decreased; still its content was the highest of all examined ions found in young leaves. This indicated that beans can develop different mechanisms to accept and adapt high levels of salt. Storing toxic ion (Na + ) in old leaves and having a limited transmission of salt into young leaves serves as a protection from detrimental effects of salt.
ABSTRACT. Green bean genotypes collected from eastern Turkey were characterized using simple sequence repeat (SSR) markers and morphological traits. Among 12 SSR markers, 10 produced successful amplifications and revealed DNA polymorphisms that were subsequently used to assess genetic relatedness of the genotypes. Based on the number of alleles generated and the probability of identity values, the most informative SSR loci were PVGLND5, PVMEIG, PVag001, and PV-ag004. Probably, due to the inbreeding nature of beans, the heterozygosity observed within genotypes was low at most of the SSR loci. The UPGMA dendrogram constructed based on the SSR data yielded two major clusters. The overall genetic distance was around 98%, among the genotypes. This information can be used to help select Turkish green bean lines.
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