Genetic Characterization of Highly Pathogenic Avian Influenza (H5N8) Virus from Domestic Ducks, England, November 2014

Hanna, Amanda; Banks, Jill; Marston, Denise A.; Ellis, Richard J.; Brookes, Sharon M.; Brown, Ian H.

doi:10.3201/eid2105.141954

Cited by 58 publications

(44 citation statements)

References 11 publications

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“…By summer 2014, the virus was detected in Japan, Beringia and Siberia [16,17]. By the end of 2014, waterfowl migration played an essential role in spreading HPAIV H5N8 in Europe, North America and East Asia [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of highly pathogenic avian influenza A H5N8 viruses isolated from wild birds in Egypt

Kandeil

Kayed

Moatasim

et al. 2017

Journal of General Virology

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A newly emerged H5N8 influenza virus was isolated from green-winged teal in Egypt during December 2016. In this study, we provide a detailed characterization of full genomes of Egyptian H5N8 viruses and some virological features. Genetic analysis demonstrated that the Egyptian H5N8 viruses are highly pathogenic avian influenza viruses. Phylogenetic analysis revealed that the genome of the Egyptian H5N8 viruses was related to recently characterized reassortant H5N8 viruses of clade 2.3.4.4 isolated from different Eurasian countries. Multiple peculiar mutations were characterized in the Egyptian H5N8 viruses, which probably permits transmission and virulence of these viruses in mammals. The Egyptian H5N8 viruses preferentially bound to avian-like receptors rather than human-like receptors. Also, the Egyptian H5N8 viruses were fully sensitive to amantadine and neuraminidase inhibitors. Chicken sera raised against commercial inactivated avian influenza-H5 vaccines showed no or very low reactivity with the currently characterized H5N8 viruses in agreement with the genetic dissimilarity. Surveillance of avian influenza in waterfowl provides early warning of specific threats to poultry and human health and hence should be continued.

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Section: Introductionmentioning

confidence: 99%

Genetic characterization of highly pathogenic avian influenza A H5N8 viruses isolated from wild birds in Egypt

Kandeil

Kayed

Moatasim

et al. 2017

Journal of General Virology

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“…Thereafter, these viruses have been circulating in both avian populations in South Korea [3,4] and sporadically in neighbouring countries, including China and Japan. Since November 2014, H5N8 HPAIVs have also appeared in poultry and wild birds in Europe [5,6]. Genetic analyses revealed that these isolates were closely related to the H5N8 viruses circulating in Korean birds.…”

mentioning

confidence: 99%

Genetic diversity of highly pathogenic H5N8 avian influenza viruses at a single overwintering site of migratory birds in Japan, 2014/15

et al. 2015

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We isolated eight highly pathogenic H5N8 avian influenza viruses (H5N8 HPAIVs) in the 2014/15 winter season at an overwintering site of migratory birds in Japan. Genetic analyses revealed that these isolates were divided into three groups, indicating the co-circulation of three genetic groups of H5N8 HPAIV among these migratory birds. These results also imply the possibility of global redistribution of the H5N8 HPAIVs via the migration of these birds next winter.

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“…In January 2014, novel reassortant HPAI viruses of subtype H5N8 were reported in South Korea, Japan and China 2 . In late 2014, H5N8 HPAI viruses spread to Europe and North America and caused outbreaks in poultry [3][4][5] .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous subtyping and pathotyping of the novel reassortant influenza A (H5N8) virus from clinical samples using a diagnostic microarray

et al. 2016

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Highly pathogenic avian influenza (HPAI) viruses cause economic losses in the poultry industry and pose a severe threat to human health. Rapid and accurate diagnostic methods are important because they can help prevent further spread of the virus and reduce the time required for eradication of the virus. We developed a low-density microarray for the rapid detection and identification of avian influenza virus subtypes H5, H7, and H9 and their pathotypes in a pre vious study. In the present study, we report the develop ment of updated probe sets and evaluation of the diag nostic microarray using H5N8 clade 2.3.4.4 HPAI viruses including clinical samples, without the need for egg propagation. Cy3-labeled DNA targets were obtained by reverse transcription polymerase chain reaction using Cy3-labeled universal primers, and labeled amplicons were hybridized to the microarray. All posi tive samples from RT-PCR showed H5-specific and highly pathogenic pattern in the microarray, without purification of PCR products. Furthermore, it allowed for specific detection of the subtype and pathotype from low DNA concentration samples that did not allow direct sequence analysis. Therefore, this diagnostic microarray has enormous potential for the rapid subtyping and pathotyping from clinical samples without the need for culture.

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Genetic Characterization of Highly Pathogenic Avian Influenza (H5N8) Virus from Domestic Ducks, England, November 2014

Cited by 58 publications

References 11 publications

Genetic characterization of highly pathogenic avian influenza A H5N8 viruses isolated from wild birds in Egypt

Genetic characterization of highly pathogenic avian influenza A H5N8 viruses isolated from wild birds in Egypt

Genetic diversity of highly pathogenic H5N8 avian influenza viruses at a single overwintering site of migratory birds in Japan, 2014/15

Simultaneous subtyping and pathotyping of the novel reassortant influenza A (H5N8) virus from clinical samples using a diagnostic microarray

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