Genetic characterization of indigenous anatolian water buffalo breed using microsatellite dna markers

Abstract

One indigenous water buffalo population to Anatolia was characterised with 11 cattle autosomal microsatellite loci. A set of 4 cattle microsatellite loci was found to be polymorphic in the Anatolian buffalo genome. Genotyping of these polymorphic microsatellite loci revealed alleles ranging from 3 to 9. The observed heterozygosity ranged from 0.550 to 0.775 and the expected heterozygosity ranged from 0.494 to 0.815. The FIS value within each locus, changed from –0.101 to 0.205. Total FIS was 0.043 indicating that Anatolian water buffalo population samples seemed to be in Hardy- Weinberg expectation

Genetic diversity of brazilian buffaloes (Bubalus bubalis) using DNA microsatellites

“…The Jafarabadi population showed the most deviation with 10 markers, while the other populations showed disequilibrium with five to eight markers. These results are in agreement with those reported by Soysal et al (2005), in Turkey and Flamand et al …”

“…The mean of observed heterozygosity (H o ) and expected heterozygosity or gene diversity (H e ) are close to those reported by Soysal et al (2005), Arora et al (2004), Flamand et al (2003) and Navani et al (2002). Moioli et al (2001) found values significantly lower in buffaloes from Greece, Italy and Egypt.…”

“…This allele mean number coincides with Soysal et al (2005) and Arora et al (2004) in buffaloes from Turkey and North of India, and Flamand et al (2003) and Barker et al (1997) in Buffaloes from Nepal, SouthEastern Asia and Australia. Four markers showed a great deficit of heterozygotes mainly in tree populations (table III).…”

“…They also found high variability within and between populations. In Turkey, Soysal et al (2005) also found a great genetic variability in the buffaloes of this country. In the opposite, Arora et al (2004) found a low level of variability in river buffalo populations in the North of India.…”

Anatolian water buffaloes husbandry in Turkey: preliminary results on somatic characterization

“…These results are a preliminary step for characterization of Anatolian buffalo. Characterization of this buffalo under various aspect is very important as the high degree of genetic variability, tested by means of microsatellite markers (Soysal et al, 2007), induces to think that there is a good perspective of genetic improvement. and suggests that this buffalo may be an important tool to increase productivity both in small farms located in villages systems and in medium-large farms.…”