Genetic characterization of L-Zagreb mumps vaccine strain

Ivančić, Jelena; Gulija, Tanja Košutić; Forčić, Dubravko; Baričević, Marijana; Jug, Renata; Mesko-Prejac, Majda; Mažuran, Renata

doi:10.1016/j.virusres.2004.11.013

Cited by 28 publications

(17 citation statements)

References 45 publications

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“…To minimize the possible discrepancies and differences between antigen content of the 3 virus samples to be compared expressed as CCID 50 (infective virus quantity) and their total antigen content that was not monitored in this study, all 3 viruses were prepared (propagated) exactly in the same way and formulated equally. JL5 (genotype A), Urabe (genotype B) and LZG (genotype N) were highly cross-reactive to each other although belonging to different genotypes 36,37 and were also cross-reactive to the wild-type virus of genotype G 37 supporting the historically accepted fact that MuV exists as a single serotype, also verified in recent years. 38,39 Irrespective of which virus was used as a hemagglutinating agent, LZG showed significantly higher immunogenicity in comparison to JL5 strain.…”

Section: Discussionsupporting

confidence: 60%

Factors influencing preclinicalin vivoevaluation of mumps vaccine strain immunogenicity

Halassy

Kurtović

Brgles

et al. 2015

Human Vaccines & Immunotherapeutics

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Keywords: design of Experiments (DoE), in vivo bioassay optimization, mumps virus, non-clinical vaccine immunogenicity assessmentImmunogenicity testing in animals is a necessary preclinical assay for demonstration of vaccine efficacy the results of which are often the basis for the decision whether to proceed or withdraw the further development of the novel vaccine candidate. However, in vivo assays are rarely, if at all, optimized and validated. Here we clearly demonstrate the importance of in vivo assay (mumps virus immunogenicity testing in guinea pigs) optimization for gaining reliable results and the suitability of Fractional factorial design of experiments (DoE) for such a purpose. By the use of DoE with resolution IV (2 IV (4-1) ) we clearly revealed that the parameters significantly increasing assay sensitivity were interval between animal immunizations followed by the body weight of experimental animals. The quantity (0 versus 2%) of the stabilizer (fetal bovine serum, FBS) in the sample was shown as non-influencing parameter in DoE setup. However, the separate experiment investigating only the FBS influence, and performed under other parameters optimally set, showed that FBS also influences the results of immunogenicity assay. Such finding indicated that (a) factors with strong influence on the measured outcome can hide the effects of parameters with modest/low influence and (b) the matrix of mumps virus samples to be compared for immunogenicity must be identical for reliable virus immunogenicity comparison. Finally the 3 mumps vaccine strains widely used for decades in the licensed vaccines were for the first time compared in an animal model, and results obtained were in line with their reported immunogenicity in human population supporting the predictive power of the optimized in vivo assay.

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Section: Discussionsupporting

confidence: 60%

Factors influencing preclinicalin vivoevaluation of mumps vaccine strain immunogenicity

Halassy

Kurtović

Brgles

et al. 2015

Human Vaccines & Immunotherapeutics

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“…The presence of mumps virus was determined by hemadsorption with 0.5 % guinea pig erythrocyte suspension and a neutralization test with mumps polyclonal sera (Institute of Immunology, Inc., Zagreb, Croatia). RT-PCR for SH gene, detection in agarose gel, sequencing of SH genes (on ABI Prism 377 automatic DNA sequencer, Applied Biosystems, Foster City, Ca, USA) and alignment with SH gene of L-Zagreb mumps vaccine strain was then done as previously described (9).…”

Section: Isolation and Determination Of Virusesmentioning

confidence: 99%

Naive and Memory CD4⁺T-cells in the Cerebrospinal Fluid of Children with Aseptic Meningitis Following Measles-Mumps-Rubella Vaccination and Enteroviral Meningitis

Lepej

Tešović

Sternak

et al. 2007

Immunological Investigations

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“…Another hypothesis we tested was uneven efficiency of PCR amplification of the two fragments [30,37]. We determined the amount of variant B in H1-H4 samples after 30, 35 and 40 PCR cycles by RFLP-CE-FLA and CE-SSCP, expecting that different results will be obtained with different number of PCR cycles.…”

Section: Discrepancy Between Rflp-ce-fla and Ce-sscp Resultsmentioning

confidence: 99%

Comparative analysis of CE‐SSCP to standard RFLP‐CE‐FLA method in quantification of known viral variants within an RNA virus quasispecies

et al. 2011

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RNA viruses display the highest replication error rate in our biosphere, leading to highly diverse viral populations termed quasispecies. The gold standard method for detection and quantification of variants in a quasispecies is cloning and sequencing, but it is expensive, laborious and time consuming. Therefore, other mutation detection approaches, including SSCP, are often used. In this study, we demonstrate development and the usage of a CE-SSCP method for quantification of two nearly identical viral variants in heterogenic population of a mumps virus strain and its comparison to RFLP-CE-fragment length analysis (RFLP-CE-FLA). Analyzed PCR fragments were of the same size (245 bp) with one difference in their nucleotide sequence. The limit of detection of both methods was at 5% of the minor variant. When PCR amplicons of the two variants were pooled, methods' results were very similar. On the contrary, the quantification results of samples in which variants were mixed prior to PCR showed substantial difference between the two methods. Our results indicate that although both methods can be used for detection and monitoring of a specific mutation within a viral population, caution should be taken when quantitative analysis of complex samples is based solely on results of one method.

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Genetic characterization of L-Zagreb mumps vaccine strain

Cited by 28 publications

References 45 publications

Factors influencing preclinicalin vivoevaluation of mumps vaccine strain immunogenicity

Factors influencing preclinicalin vivoevaluation of mumps vaccine strain immunogenicity

Naive and Memory CD4⁺T-cells in the Cerebrospinal Fluid of Children with Aseptic Meningitis Following Measles-Mumps-Rubella Vaccination and Enteroviral Meningitis

Comparative analysis of CE‐SSCP to standard RFLP‐CE‐FLA method in quantification of known viral variants within an RNA virus quasispecies

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Genetic characterization of L-Zagreb mumps vaccine strain

Cited by 28 publications

References 45 publications

Factors influencing preclinicalin vivoevaluation of mumps vaccine strain immunogenicity

Factors influencing preclinicalin vivoevaluation of mumps vaccine strain immunogenicity

Naive and Memory CD4+T-cells in the Cerebrospinal Fluid of Children with Aseptic Meningitis Following Measles-Mumps-Rubella Vaccination and Enteroviral Meningitis

Comparative analysis of CE‐SSCP to standard RFLP‐CE‐FLA method in quantification of known viral variants within an RNA virus quasispecies

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Product

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About

Naive and Memory CD4⁺T-cells in the Cerebrospinal Fluid of Children with Aseptic Meningitis Following Measles-Mumps-Rubella Vaccination and Enteroviral Meningitis