Genetic characterization of  Orobanche cumana  populations from the Thrace region of  Turkey using microsatellite markers

Bilgen, Behiye Banu; Barut, Ahmet Kubilay; Demirbaş, Sefer

doi:10.3906/bot-1807-71

Cited by 9 publications

(17 citation statements)

References 30 publications

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“…Overall, the observed heterozygosity was lower than the expected heterozygosity among the three data set, which generate a positive F IS value for the majority of the studied populations indicating an inbreeding behavior of the O. foetida populations in Tunisia. Similar results were also recorded among O. cumana populations from Turkey (Bilgen et al, 2019), Spain andBulgaria (Pineda-Martos et al, 2013, 2014a) using SSR markers, pointing out a deviation from Hardy-Weinberg equilibrium. Furthermore, studies conducted on Orobanche spp.…”

Section: Genetic Diversitysupporting

confidence: 80%

“…Nevertheless, comparison among diversity studies is more conclusive using the same species and the same type of markers. Indeed, Bilgen et al (2019) used 8 SSR markers to study O. cumana populations from Turkey. They reported a variation regarding mean values of Ne (1.67), Ho (0.21), He (0.34) and the Fixation index (F) (0.34) and were able to compare their results with those of Pineda-Martos et al (2013) in terms of heterozygosity levels in populations of O. cumana from Spain, Bulgaria and Turkey.…”

Section: Genetic Diversitymentioning

confidence: 99%

“…In fact, several studies were conducted to analyze the genetic diversity in Orobanche genera for several species using different techniques mainly; isoenzymes for O. crenata (Verkleij et al, 1986) and O. cumana (Castejon Munoz et al, 1991), RAPD for O. crenata (Román et al, 2001b), O. cumana (Gagne et al, 1998), O. gracilis (Román et al, 2007a) and O. ramosa (Brault et al, 2007), ISSR for O. minor (Westwood and Fagg, 2004), AFLP for O. cumana (Gagne et al, 2000). Ever since, specific markers were developed for some species such SSR for O. cumana by Pineda-Martos et al (2014b) which offers the opportunity to study O. cumana diversity in many countries; Spain (Pineda-Martos et al, 2013;Martín-Sanz et al, 2016), Tunisia (Jebri et al, 2017) and Turkey (Bilgen et al, 2019). These markers were also used recently to study the genetic diversity of O. crenata populations from Ethiopia (Belay et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Genetic Diversity and Population Structure of Orobanche foetida Populations From Tunisia Using RADseq

et al. 2021

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Orobanche foetida Poiret is a holoparasitic plant that lacks chlorophyll and totally depending on its host for its growth. Orobanche foetida parasitizes host plant roots and extract nutrient and water via a haustorium. Although O. foetida distributes in the Mediterranean region as a wild plant parasite, it parasitizes faba bean causing serious damages which may reach 90% yield losses in Tunisia. Analysis of genetic diversity of the parasite is important to better understand its evolution and spread, remained largely unknown. In this work, we present the first study on genetic diversity and population structure using the robust technique Restriction-site-Associated DNA sequencing (RADseq) for Orobanche spp. We collected 244 samples of O. foetida from 18 faba bean fields in the north of Tunisia including 17 populations from the north-west and one population form the north-east. To overcome the difficulty of SNP discovery in O. foetida genome as a non-model and tetraploid plant, we utilized three different informatics pipelines, namely UNEAK, pyRAD and Stacks. This study showed that genetic differentiation occurred in the Tunisian O. foetida emphasizing the isolation by distance effect. However, no strong population clustering was detected in this work basing on the three data sets and clustering methods used. The present study shed the light on the current distribution and the genetic variation situation of the fetid broomrape in Tunisia, highlighting the importance of understanding the evolution of this parasite and its genetic background. This will aid in developing efficient strategies to control this parasite and its expansion in Tunisia and worldwide.

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Section: Genetic Diversitysupporting

confidence: 80%

Section: Genetic Diversitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Genetic Diversity and Population Structure of Orobanche foetida Populations From Tunisia Using RADseq

et al. 2021

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“…Due to their codominant, hypervariable, and reliable scoring characteristics, SSR markers have high efficiency in population genetic studies (Yang et al, 2015;Nisar et al, 2017; Bilgen et al, 2019). In this study, a set of microsatellite loci was developed to examine the population genetics of F. sinkiangensis.…”

Section: Polymorphic and Highly Informative 14 Microsatellite Loci For F Sinkiangensismentioning

confidence: 99%

“…The best molecular markers suited for detecting genetic variability should be relatively easy to implement, inexpensive, and polymorphic enough. As a codominant and polymorphism genetic marker, microsatellites (SSRs) have been proven to be a reliable technique, capable of detecting the genetic diversity and structures of rare and endangered plants (Yang et al, 2015;Nisar et al, 2017;Bilgen et al, 2019). In order to implement reasonable conservation strategies and promote effective management practices, the genetic diversity of F. sinkiangensis was researched.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the critically endangered Ferula sinkiangensis K. M. Shen (Apiaceae) and the implications for conservation

Yang

et al. 2020

Turk J Bot

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Research on the genetic diversity and structures of endangered plant species with small population sizes is a prerequisite for developing reasonable conservation strategies. Ferula sinkiangensis K.M.Shen is a critically endangered herbaceous perennial and monocarpic medical plant growing in semiarid steppe ecosystems with a small population size within the Yili Valley in Xinjiang, China. Unfortunately, the lack of genetic information has limited our ability to initiate effective conservation strategies for the species. In this study, 14 polymorphic microsatellite primers of F. sinkiangensis were developed and characterized using Illumina HiSeq paired-end reads from genomic DNA. Primers were used to assess the genetic diversity and structure of the species in the Yili Valley. A total of 92 alleles were detected across 14 microsatellite loci from 65 specimens of F. sinkiangensis. Moderate to high levels of genetic diversity (He = 0.446, I = 0.821, PPB = 92.86%) were found within the species. Our results contradict the general hypothesis that rare and endangered species with small population sizes and narrow ranges of distribution exhibit low levels of genetic diversity. The moderate to high levels of genetic diversity within the species can be explained by the mating system, life history traits, and human activities in recent years. Several reasonable conservation and reintroduction strategies are proposed, including maintenance of the plant's natural habitats, seeds or seedlings collection for germplasm storage, and artificial breeding of the species.

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Inferring the biogeography of New Zealand’s only endemic holoparasitic plant, the threatened Dactylanthus taylorii (Mystropetalaceae)

McLay

Tate

Gemmill

et al. 2022

New Zealand Journal of Botany

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Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers

Abstract: Introduction Sunflower (Helianthus annuus L.) is one of the most significant oilseed crops in Europe, mainly grown for its edible oil and confectionery uses. Turkey takes part in the second group of sunflower producer countries (totally 27%) with China,

Cited by 9 publications

References 30 publications

Analysis of Genetic Diversity and Population Structure of Orobanche foetida Populations From Tunisia Using RADseq

Analysis of Genetic Diversity and Population Structure of Orobanche foetida Populations From Tunisia Using RADseq

Genetic diversity of the critically endangered Ferula sinkiangensis K. M. Shen (Apiaceae) and the implications for conservation

Inferring the biogeography of New Zealand’s only endemic holoparasitic plant, the threatened Dactylanthus taylorii (Mystropetalaceae)

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