Genetic conservation of Ficus bonijesulapensis R.M. Castro in a dry forest on limestone outcrops

Duarte, Jaqueline Fidelis; Carvalho, Dulcinéia de; Vieira, Fábio de Almeida

doi:10.1016/j.bse.2015.01.008

Cited by 18 publications

(16 citation statements)

References 52 publications

(31 reference statements)

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“…In the present study, the average PIC was 0.44, and so we can consider that the ISSR primers were moderately informative to reveal polymorphism in the jatobá individuals; this confirms that the markers utilized were efficient to estimate genetic diversity in H. courbaril since ISSR classified as moderately informative have been successfully used in other species, e.g., Ficus bonijesulapensis R.M. Castro (Duarte et al, 2015), Hancornia speciosa Gomes (Costa et al 2015), Mimosa caesalpiniaefolia Benth. (Araújo et al, 2016), and Theobroma grandiflorum (Willd.…”

Section: Resultssupporting

confidence: 52%

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Rocha¹,

Tiago²,

Tiago³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Hymenaea courbaril L. is a tropical tree species with economic and medicinal potential largely used in programs for the recovery of degraded areas. The use of molecular markers in genetic studies can 2 V.D. Rocha et al. Genetics and Molecular Research 16 (3): gmr16039706 help in the definition of forest restoration strategies. This study was conducted to analyze the genetic diversity among H. courbaril ('jatobá') genotypes naturally occurring in the north of Mato Grosso State, Brazil, using ISSR markers, aiming to contribute to the development of seed collection and seedling production strategies. Twenty-four H. courbaril individuals were sampled by collecting leaves for DNA extraction by the CTAB method. The individuals were genotyped using 14 ISSR primers, resulting in the amplification of 87 bands in total, 65 of which were polymorphic. The ISSR markers presented average polymorphism information content of 0.44. Dissimilarity values based on the Jaccard coefficient ranged from 0.12 to 0.52. The most divergent individuals were 3 and 22, whereas 14 and 19 were the most genetically similar. The UPGMA and Tocher clustering methods indicated the formation of four distinct groups, revealing genetic variability among the 24 H. courbaril genotypes. Therefore, all the evaluated genotypes can be used for seed collection and seedling production, but the most divergent individuals should be prioritized.

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Section: Resultssupporting

confidence: 52%

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Rocha¹,

Tiago²,

Tiago³

et al. 2017

Genet. Mol. Res.

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“…Devido à herança dominante, a presença do fragmento (banda) pode representar o homozigoto dominante ou o heterozigoto, enquanto a ausência pode ser infligida o estado homozigoto recessivo, à ocorrência de inserções/deleções, ou à adição ou perda de um sítio de reconhecimento do primer (Zietkiewicz et al, 1994). Apesar do uso contínuo de marcadores ISSR (Brandão et al, 2011;Duarte et al, 2015;Melo Junior et al, 2015), ainda são escassos os estudos envolvendo a metodologia de seleção e indicação do número mínimo de locos para uso na genética de populações (Chagas et al, 2015;Fajardo et al, 2014;Mendonça et al, 2013).…”

Section: Introductionunclassified

Diversidade genética de Copernicia prunifera com o uso de marcadores moleculares ISSR

Vieira

Sousa

Silva

et al. 2015

Agraria

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RESUMOO objetivo desse estudo foi selecionar primers ISSR (Inter Simple Sequence Repeat) para estudos da estrutura genética, assim como quantificar a variabilidade genética de uma população natural da C. prunifera. (UBC 841). O número ótimo de locos para se estimar confiavelmente a diversidade genética para esse trabalho foi de 76 locos. Foi encontrada alta diversidade genética, com o número de alelos observados (na = 2,00), alelos efetivos (ne = 1,46), índice de diversidade de Nei (He = 0,28) e índice de Shannon (Ho = 0,44). O valor da coancestria se manteve dentro do intervalo de confiança (P > 0,05), indicando ausência de estrutura genética espacial. Os marcadores ISSR se mostraram eficientes na caracterização genotípica dos indivíduos de carnaúba, servindo como subsídio para planos de manejo e conservação da espécie.Palavras-chave: Arecaceae, carnaúba, nordeste do Brasil, variação genética Genetic diversity of Copernicia prunifera using ISSR molecular markers ABSTRACTThe aim of this study was to select primers ISSR (Inter Simple Sequence Repeat) for studies of the genetic structure, and to quantify the genetic variability in a natural population of C. prunifera. Thirty seven individuals were sampled in the municipality Macaíba, Rio Grande do Norte, Northeast Brazil. Seventeen ISSR primers were tested, of which 12 amplified DNA and of these, seven were selected to characterize the structure and genetic diversity of the population. The primers that had the highest percentage of polymorphic loci were UBC 841 (16.36%), UBC 842 (15.45%), UBC 857 (12.73%), UBC 859 (10.90%), UBC 840 (10.90%), UBC 813 (10%) and UBC 827 (8.18%), totaling 93 of the 110 loci generated. The polymorphic information content (PIC) of each selected primer ranged from 0.057 (UBC 859) and 0.444 (UBC 841). The optimal number of loci to reliably estimate the genetic diversity for this work was 76 loci, ie, when the correlation reached 0.998 and the stress was less than 0.05. High genetic diversity was found, with the number of observed alleles (na = 2.00), effective alleles (ne = 1.46), Nei's diversity index (He = 0.28) and Shannon index (Ho = 0.44). The value of the coancestry remained within the confidence interval (P > 0.05), indicating no spatial genetic structure, regardless of the distance between plants. ISSR markers were efficient in genetic characterization of individuals' carnauba serving with allowance for management and conservation of the species.

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“…Tree species that regenerate on rocky cliffs, such as Ficus spp. and Dracaena spp., usually have specialized root systems that are able to thread through the rocky substratum and extract nutrients from the thin soil/humus layer (Denk et al 2014;Duarte et al 2015). The roots commonly grow deep into the rock fissures of the epikarst substratum (Huang et al 2011).…”

Section: Environmental Factors Affecting Tree Regenerationmentioning

confidence: 99%

Environmental Factors Influencing Tree Species Regeneration in Different Forest Stands Growing on a Limestone Hill in Phrae Province, Northern Thailand

Asanok¹,

Marod²

2016

Journal of Forest and Environmental Science

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Improved knowledge of the environmental factors affecting the natural regeneration of tree species in limestone forest is urgently required for species conservation. We examined the environmental factors and tree species characteristics that are important for colonization in diverse forest stands growing on a limestone hill in northern Thailand. Our analysis estimated the relative influence of forest structure and environmental factors on the regeneration traits of tree species. We established sixty-four 100-m 2 plots in four forest stands on the limestone hill. We determined the species composition of canopy trees, regenerating seedlings, and saplings in relation to the physical environment. The relationships between environmental variables and tree species abundance were assessed by canonical correspondence analysis (CCA), and we used generalized linear mixed models to examine data on seedling/sapling abundances. The CCA ordination indicated that the abundance of tree species within the mixed deciduous forest was closely related to soil depth. The abundances of tree species growing within the sink-hole and hill-slope stands were positively related to the extent of rocky outcropping; light and soil moisture positively influenced the abundance of tree species in the hill-cliff stand. Physical factors had a greater effect on tree regeneration than did factors related to forest structure.Tree species, such as Ficus macleilandii, Dracaena cochinchinensis, and Phyllanthus mirabilis within the hill-cliff or sink-hole stand, colonized well on large rocky outcroppings that were well illuminated and had soft soils. These species regenerated well under conditions prevailing on the limestone hill. The colonization of several species in other stands was negatively influenced by environmental conditions at these sites. We found that natural regeneration of tree species on the limestone hill was difficult because of the prevailing combination of physical and biological factors. The influence of these factors was species dependent, and the magnitude of effects varied across forest stands.

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Genetic conservation of Ficus bonijesulapensis R.M. Castro in a dry forest on limestone outcrops

Cited by 18 publications

References 52 publications

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Diversidade genética de Copernicia prunifera com o uso de marcadores moleculares ISSR

Environmental Factors Influencing Tree Species Regeneration in Different Forest Stands Growing on a Limestone Hill in Phrae Province, Northern Thailand

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