Genetic control of early events in protoplast division and regeneration pathways in sunflower

Berrios, E. Flores; Gentzbittel, Laurent; Mokrani, L.; Alibert, Gilbert; Sarrafi, A.

doi:10.1007/s001220051522

Cited by 17 publications

(2 citation statements)

References 28 publications

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“…Transgressive segregation has already been described in other reports in relation to the genetic control of plant regeneration [31-33], and suggests poligenic inheritance [34]. It also suggests the existence of alleles that promote, and others that inhibit, in vitro regeneration, with only some of the alleles with positive effects occurring in the same parent [34].…”

Section: Discussionmentioning

confidence: 81%

Localization of QTLs for in vitroplant regeneration in tomato

et al. 2011

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BackgroundLow regeneration ability limits biotechnological breeding approaches. The influence of genotype in the regeneration response is high in both tomato and other important crops. Despite the various studies that have been carried out on regeneration genetics, little is known about the key genes involved in this process. The aim of this study was to localize the genetic factors affecting regeneration in tomato.ResultsWe developed two mapping populations (F2 and BC1) derived from a previously selected tomato cultivar (cv. Anl27) with low regeneration ability and a high regeneration accession of the wild species Solanum pennellii (PE-47). The phenotypic assay indicated dominance for bud induction and additive effects for both the percentage of explants with shoots and the number of regenerated shoots per explant. Two linkage maps were developed and six QTLs were identified on five chromosomes (1, 3, 4, 7 and 8) in the BC1 population by means of the Interval Mapping and restricted Multiple QTL Mapping methods. These QTLs came from S. pennellii, with the exception of the minor QTL located on chromosome 8, which was provided by cv. Anl27. The main QTLs correspond to those detected on chromosomes 1 and 7. In the F2 population, a QTL on chromosome 7 was identified on a similar region as that detected in the BC1 population. Marker segregation distortion was observed in this population in those areas where the QTLs of BC1 were detected. Furthermore, we located two tomato candidate genes using a marker linked to the high regeneration gene: Rg-2 (a putative allele of Rg-1) and LESK1, which encodes a serine/threonine kinase and was proposed as a marker for regeneration competence. As a result, we located a putative allele of Rg-2 in the QTL detected on chromosome 3 that we named Rg-3. LESK1, which is also situated on chromosome 3, is outside Rg-3. In a preliminary exploration of the detected QTL peaks, we found several genes that may be related to regeneration.ConclusionsIn this study we have identified new QTLs related to the complex process of regeneration from tissue culture. We have also located two candidate genes, discovering a putative allele of the high regeneration gene Rg-1 in the QTL on chromosome 3. The identified QTLs could represent a significant step toward the understanding of this process and the identification of other related candidate genes. It will also most likely facilitate the development of molecular markers for use in gene isolation.

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Section: Discussionmentioning

confidence: 81%

Localization of QTLs for in vitroplant regeneration in tomato

et al. 2011

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“…As is well known, asymmetrical division is the earliest event in embryos forming from protoplasts. Therefore general quantity of reveals on 100 protoplasts and asymmetrical divisions on 100 protoplasts in 52 recombinant inbred lines from crosses of sunflower lines PAC-2 and RHA-266 in protoplast culture were investigated (Flores Berrios et al, 2000). It was revealed that 12 loci were associated with general quantity of reveals on 100 protoplasts and 11 loci with asymmetrical divisions on 100 protoplasts.…”

Section: Introductionmentioning

confidence: 99%

Morphogenesis of in vitro cultured tissues and immature germs of sunflower hybrids (Helianthus annuus L.)

Marchenko¹

2005

Helia

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Cotyledons, roots, hypocotyls and immature germs of sunflower hybrids have been tested for morphogenesis. High morphogenetic ability was revealed in the cotyledon explants, hypocotyls and immature germs. Explants of hybrids Poglad, Kiy and Svitoch showed the best ability for morphogenesis. The obtained shoots were rooted for development of complete plants. The effect of "premature flowering" was discovered in all cases.

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Genomics as Efficient Tools: Example Sunflower Breeding

Sarrafi

Gentzbittel

Biotechnology in Agriculture and Forestry

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Genetic control of early events in protoplast division and regeneration pathways in sunflower

Cited by 17 publications

References 28 publications

Localization of QTLs for in vitroplant regeneration in tomato

Localization of QTLs for in vitroplant regeneration in tomato

Morphogenesis of in vitro cultured tissues and immature germs of sunflower hybrids (Helianthus annuus L.)

Genomics as Efficient Tools: Example Sunflower Breeding

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