Localization of QTLs for in vitroplant regeneration in tomato

Trujillo-Moya, Carlos; Gisbert, Carmina; Vilanova, Santiago; Nuez, F.

doi:10.1186/1471-2229-11-140

Cited by 33 publications

(24 citation statements)

References 57 publications

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“…The mean number of shoots per explant with shoots (PR) proved to be higher in S. pennellii than in F1, giving 5.05 and 3.40 shoots per explant, respectively (Table 1). These results are in accordance with those previously obtained (Trujillo-Moya et al 2011).…”

Section: Effect On Organogenesis Of Different Doses Of Ethylene Inhibsupporting

confidence: 83%

The influence of ethylene and ethylene modulators on shoot organogenesis in tomato

Trujillo-Moya

Gisbert

2012

Plant Cell Tiss Organ Cult

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Abstract:The influence of ethylene and ethylene modulators on the in vitro organogenesis of tomato was studied using a highly regenerating accession of the wild tomato Solanum pennellii and an F1 plant resulting from a cross between Solanum pennellii and Solanum lycopersicum cv. Anl27, which is known to have a low regeneration frequency. Four ethylene-modulating compounds, each at four levels, were used, namely: cobalt chloride (CoCl2), which inhibits the production of ethylene; AgNO3 (SN), which inhibits ethylene action; and Ethephon and the precursor 1-aminocyclopropane-1-carboxylic acid (ACC), which both promote ethylene synthesis. Leaf explants of each genotype were incubated on shoot induction medium (SIM) supplemented with each of these compounds at 0, 10 or 15 days following bud induction. The results obtained in our assays indicate that ethylene has a significant influence on tomato organogenesis. Concentrations of ethylene lower than the optimum (according to genotype) at the beginning of the culture may decrease the percentage of explants with buds (B), produce a delay in their appearance, or indeed inhibit bud formation. This was observed in S. pennellii and the F1 explants cultured on media with SN (5.8-58.0 µM) as well as in the F1 explants cultured on medium with 21.0 µM CoCl2. The percentage of explants with shoots (R) and the mean number of shoots per explant with shoots (PR) also diminished in media that contained SN. Shoots isolated from these explants were less developed compared to those isolated from control explants. On the other hand, ethylene supplementation may contribute to enhancing shoot development. The number of isolable shoots from S. pennellii explants doubled in media with ACC (9.8-98.0 µM). Shoots isolated from explants treated with ethylene releasing compounds showed a higher number of nodes when ACC and Ethephon were added at 10 days (in F1 explants) or at 15 days (in S. pennellii) after the beginning of culture. Thus, the importance of studying not only the concentration but also the timing of the application of regulators when developing regeneration protocols has been made manifest. An excess of ethylene supplementation may produce an inhibitory effect, as was observed when using Ethephon (17.2-69.0 µM). These results show the involvement of ethylene in tomato organogenesis and lead us to believe that ethylene supplementation may contribute to enhancing regeneration and shoot development in tomato.Response to Reviewers: Dear Dr. Schuyler, Attached please find the revised version of our manuscript TICU5251, "The role of Powered by Editorial Manager® and Preprint Manager® from Aries Systems Corporationethylene in tomato shoot regeneration," which we would like to publish in Plant Cell, Tissue and Organ Culture. The revised version has been prepared taking into account your comments and those of the reviewers. Below, we have provided a detailed list of the changes made in response to the reviewers' comments. Looking forward to hearing from you. Best Regards, Carmina GisbertAns...

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Section: Effect On Organogenesis Of Different Doses Of Ethylene Inhibsupporting

confidence: 83%

The influence of ethylene and ethylene modulators on shoot organogenesis in tomato

Trujillo-Moya

Gisbert

2012

Plant Cell Tiss Organ Cult

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“…Specifically, F 2 progeny from hybrids between SC and SI species show preferential transmission of ui6.1-linked markers from the SI parent (Graham 2005;Trujillo-Moya et al 2011). In contrast, F 2 progeny from interspecific SC 3 SC crosses (i.e., lacking functional S-RNase) show normal Mendelian segregation ratios around ui6.1 (van Heusden et al 1999;.…”

Section: Discussionmentioning

confidence: 99%

The Role of a Pollen-Expressed Cullin1 Protein in Gametophytic Self-Incompatibility in Solanum

Chetelat

2014

Genetics

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We previously isolated a pollen factor, ui6.1, which encodes a Cullin1 protein (CUL1) that functions in unilateral interspecific incompatibility (UI) in Solanum. Here we show that CUL1 is also required for pollen function in self-incompatibility (SI). We used RNA interference (RNAi) to reduce CUL1 expression in pollen of Solanum arcanum, a wild SI tomato relative. Hemizygous T 0 plants showed little or no transmission of the transfer DNA (T-DNA) through pollen when crossed onto nontransgenic SI plants, indicating that CUL1-deficient pollen are selectively eliminated. When crossed onto a related self-compatible (SC) accession lacking active S-RNase, pollen transmission of the T-DNA followed Mendelian ratios. These results provide further evidence for functional overlap between SI and UI on the pollen side and suggest that CUL1 mutations will reinforce SI-to-SC transitions in natural populations only if preceded by loss of pistil S-RNase expression.S ELF-INCOMPATIBILITY (SI) is a widespread genetic mechanism in hermaphroditic plants that allows for the recognition and rejection of closely related pollen to prevent inbreeding. The breakdown of SI to self-compatibility (SC) through mutation occurs frequently (Igic et al. 2008), presumably driven by reproductive assurance under conditions where pollen from compatible mates is limiting. Pollen from SC species or populations is typically rejected on pistils of related SI species or populations, while, in the reciprocal crosses (SC pollinated by SI), no pollen rejection occurs. This pattern of unilateral incompatibility (UI) is known as the "SI 3 SC rule" (Lewis and Crowe 1958). While the mechanisms underlying SI have been the subject of much investigation, pollen rejection by UI is less well understood.The cultivated tomato (Solanum lycopersicum) and related wild Solanum species provide a powerful system with which to study these reproductive barriers. They exhibit a wide range of mating systems, including SI-enforced obligate outcrossing, SC with facultative outcrossing, and SC with high levels of inbreeding (Rick 1988). Self-compatible biotypes or accessions of mostly SI species provide a source of natural variation for studying SI-related factors.Self-incompatibility in Solanum and other Solanaceae is the S-RNase based, gametophytic type, in which S-specificity is determined by S-RNases in the pistil (McClure et al. 1989) and S-locus F-box proteins (SLFs) in pollen (Sijacic et al. 2004). F-box proteins, together with Skp1 and Cullin1 proteins, are components of Skp, Cullin, Fbox type (SCF) ubiquitin E3 ligases that mark proteins for degradation by the 26S proteasome (Zheng et al. 2002;Moon et al. 2004). The ubiquitinproteasome pathway is thought to regulate pollen-side SI responses in the Solanaceae (Zhang et al. 2009). In the "collaborative non-self-recognition" model (Kubo et al. 2010), the S-locus encodes multiple SLF proteins that together recognize different suites of S-RNases. In compatible pollinations, SLF/SRNase interactions lead to protection of p...

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“…Unfortunately, shoot regeneration is not always easy to achieve: among plant species, varieties, and cultivars, it is highly variable and currently unpredictable. The impact of shoot regeneration for horticulture and agriculture is illustrated by the numerous studies that assess the natural allelic variation and map quantitative trait loci (QTL) for the regeneration capacity in diverse crops, such as tomato (Solanum lycopersicum), wheat (Triticum aestivum), rice (Oryza sativa), barley (Hordeum vulgare), sunflower (Helianthus annuus), cabbage (Brassica oleracea), and potato (Solanum tuberosum) (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). However, it is difficult to draw general conclusions from these studies because of the lowresolution linkage maps and little detailed knowledge about gene functions in these crops.…”

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confidence: 99%

Combining linkage and association mapping identifiesRECEPTOR-LIKE PROTEIN KINASE1as an essentialArabidopsisshoot regeneration gene

Motte

Vercauteren

Depuydt

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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De novo shoot organogenesis (i.e., the regeneration of shoots on nonmeristematic tissue) is widely applied in plant biotechnology. However, the capacity to regenerate shoots varies highly among plant species and cultivars, and the factors underlying it are still poorly understood. Here, we evaluated the shoot regeneration capacity of 88 Arabidopsis thaliana accessions and found that the process is blocked at different stages in different accessions. We show that the variation in regeneration capacity between the Arabidopsis accessions Nok-3 and Ga-0 is determined by five quantitative trait loci (QTL): REG-1 to REG-5. Fine mapping by local association analysis identified RECEPTOR-LIKE PROTEIN KINASE1 (RPK1), an abscisic acid-related receptor, as the most likely gene underlying REG-1, which was confirmed by quantitative failure of an RPK1 mutation to complement the high and low REG-1 QTL alleles. The importance of RPK1 in regeneration was further corroborated by mutant and expression analysis. Altogether, our results show that association mapping combined with linkage mapping is a powerful method to discover important genes implicated in a biological process as complex as shoot regeneration.regeneration recalcitrance | SNP | ABA | natural variation | QTG

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Localization of QTLs for in vitroplant regeneration in tomato

Cited by 33 publications

References 57 publications

The influence of ethylene and ethylene modulators on shoot organogenesis in tomato

The influence of ethylene and ethylene modulators on shoot organogenesis in tomato

The Role of a Pollen-Expressed Cullin1 Protein in Gametophytic Self-Incompatibility in Solanum

Combining linkage and association mapping identifiesRECEPTOR-LIKE PROTEIN KINASE1as an essentialArabidopsisshoot regeneration gene

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