Genetic Control of Mixed Leukocyte Culture Reactivity

Bach, Fritz H.; Bach, Marilyn L.; Sondel, Paul M.; Sundharadas, G.

doi:10.1111/j.1600-065x.1972.tb00052.x

Cited by 20 publications

(17 citation statements)

References 39 publications

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“…Non-H2 different congenic combinations generally do not result in stimulation (29). However, an increasing number of exceptions to this generalization have been recognized, and specific loci responsible for mixed lymphocyte cultures have been postulated (30,31). One of the exceptions is found in the stimulation of BALB/c (H2d) lymphocytes by DBA/2 (H2d) cells (18,19).…”

Section: Discussionmentioning

confidence: 99%

Enumeration of Activated Thymus-Derived Lymphocytes by the Virus Plaque Assay

Kano

Bloom

Howe

1973

Proc. Natl. Acad. Sci. U.S.A.

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Lymphocytes activated by antigens or mitogens acquire the capacity to replicate viruses, and the number of activated lymphocytes can be estimated by the virus plaque assay. Concanavalin A and pokeweed mitogen produced 33-fold and 17-fold increases in virus plaqueforming cells (V-PFC), respectively, above background, while lipopolysaccharide produced only a 2-to 3-fold increase. T (thymus-derived lymphocyte)-depleted lymphocyte populations, derived from anti-O-treated or nude (athymic) mouse spleens, failed to produce V-PFC after culture with concanavalin A or pokeweed mitogen. The present studies thus demonstrate that the virus plaque assay measures activated T-lymphocytes.A dissociation between the V-PFC response and cell proliferation was previously observed in antigen-stimulated cells cultured in the presence of mitotic inhibitors.In the present studies, while stimulation of CBA (H2k) lymphocytes by DBA/2 (H2d) cells produced high levels of thymidine incorporation, lymphocyte target-cell cytotoxicity, and V-PFC, stimulation of BALB/c (H2d) lymphocytes against DBA/2 (H2d) cells resulted in even higher levels of thymidine incorporation with a virtual absence of cytotoxic lymphocytes or V-PFC. These results indicate that proliferation is not a sufficient condition for permitting lymphocytes either to exert cytotoxicity on target cells or to replicate viruses, and suggest that there may be a correlation between the development of V-PFC and cytotoxic lymphocytes. They are consistent with the view that there are at least two functional subpopulations of T-lymphocytes.

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Section: Discussionmentioning

confidence: 99%

Enumeration of Activated Thymus-Derived Lymphocytes by the Virus Plaque Assay

Kano

Bloom

Howe

1973

Proc. Natl. Acad. Sci. U.S.A.

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“…A disparity exists between the findings on the one hand of a normal response to PHA (in other than the highest dosage), which suggests adequate T-lymphocyte function, and on the other hand of an impaired response in the MLC, which indicates defective T-lymphocyte function (18). The response in the MLC may be a more sensitive indicator of T-lymphocyte function than the PHA response, or the response in the MLC and that to PHA may assay different subpopulations of T lymphocytes.…”

Section: Hiitteroth and S D Litwin Manuscript In Preparation)mentioning

confidence: 95%

Abnormal immune responses of Bloom's syndrome lymphocytes in vitro.

Hütteroth¹,

Litwin²,

German³

1975

J. Clin. Invest.

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“…In contrast, antigens coded by the I region of the MHC appear preferentially to induce proliferative responses in the MLC, yet represent poor targets for cytotoxic lymphocytes [6, 9, lo]. The interpretation of these paradoxical findings have so far resulted in at least two mutually exclusive concepts: Bach and his associates postulated that antigens coded by the I region are very efficient lymphocyte activating determinants (LAD), yet the T cell blasts produced are incapable to act as cytotoxic effector cells, this being a feature restricted to anti-SD murine T lymphocytes [3,91. This concept has been challenged by Klein et al, who postulated that each segment within the MHC, that is H-2K, H-2D, and the subregions of the I segment, all are coding for antigens capable of inducing both a MLC and t o induce CTL [ 11, 121.…”

Section: Introductionmentioning

confidence: 99%

T cell proliferation in the mixed lymphocyte culture does not necessarily result in the generation of cytotoxic T effector cells

et al. 1975

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It was tested whether T lymphocytes, when stimulated in vitro by M locus-coded lymphocyte activating determinants (LAD), are able to mediate cytotoxic effector functions. The assay for cytotoxicity included both the use of purified appropriate target cells (i.e. purified lipopolysaccharide blasts) as well as the use of phytohemagglutinin dependent cytolysis as a model for detecting cytotoxic T lymphocytes (CTL). Although strong proliferative responses were obtained in the mixed lymphocyte culture, the T cell blast generated did not display any detectable cytotoxic effector function. Thus, it is concluded that LAD, at least in the M locus-dependent system, do have the capacity to induce T cell proliferation but do not induce CTL.

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Genetic Control of Mixed Leukocyte Culture Reactivity

Cited by 20 publications

References 39 publications

Enumeration of Activated Thymus-Derived Lymphocytes by the Virus Plaque Assay

Enumeration of Activated Thymus-Derived Lymphocytes by the Virus Plaque Assay

Abnormal immune responses of Bloom's syndrome lymphocytes in vitro.

T cell proliferation in the mixed lymphocyte culture does not necessarily result in the generation of cytotoxic T effector cells

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