Genetic Data Concerning Shigella flexneri Serotypes 5 and 6

Timakov, V. D.; Petrovskaya, V. G.; Bondarenko, V. M.; Khomenko, N. A.

doi:10.1099/00207713-22-3-149

Cited by 11 publications

(11 citation statements)

References 5 publications

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“…This is in accord with the findings of many workers that S.jexneri serotype 6 strains differ from other strains of the species in their biochemical reactions. They are known to differ in their phage and colicin sensitivity patterns, their lipopolysaccharide structures, in the gene loci determining their antigens and in their esterases (Timakov et al, 1972;Simmons, 1971 ;Katzenellenbogen et al, 1976;Goullet, 1980). Various workers have suggested the removal of strains designated S.fiexneri serotype 6 from the species S.flexneri (Timakov et al, 1972;Petrovskaya & Bondarenko, 1977;Slopek & Mulczyk, 1967;Gekker et al, 1965;Simmons, 1971 ;Goullet, 1980).…”

Section: Numerical Taxonomy Of Genus Shigellamentioning

confidence: 99%

A Numerical Taxonomic Study of the Genus Shigella

Dodd

Jones

1982

Microbiology

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One hundred and two strains representing the four species of the genus Shigella and sixty-four strains representing fourteen other genera of the family Enterobacteriaceae were tested for 192 characters based on their morphology, biochemistry and physiology. The data were subjected to a number of numerical analyses. The results (confirmed by the use of overlap statistics) showed that four phenons can be distinguished within the genus Shigella. These correspond with the species S . dysenteriae, S. flexneri, S. boydii and S . sonnei.Of the other bacteria studied the Alkalescens-Dispar group was most closely related to the genus Shigella and the need for this group to be studied further is noted. The importance of using computer-based matrices for the routine identification of strains of Shigella and other enterobacteria is discussed.Strains of the genus Providencia clustered separately from the shigellae. Three distinct species were evident within this genus: P . stuartii, P . alcalifaciens and a new Providencia species. The latter can be equated with the 'BG3' group in the DNA-DNA hybridization studies of Brenner et al. (1978).

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Section: Numerical Taxonomy Of Genus Shigellamentioning

confidence: 99%

A Numerical Taxonomic Study of the Genus Shigella

Dodd

Jones

1982

Microbiology

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“…Strains of S. boydii serotype 13, which belong to a discrete DNA hybridization group (5), were clearly distinguished from the other Shigella strains. Rolland et al (31) could not demonstrate the close taxonomic relationship between S. flexneri serotype 6 strains and S. boydii strains, but this association was proven on the basis of antigenic, chemical, and genetic data (10,29,30,34). Another ribotyping system based on restriction with endonuclease MluI was proposed (8).…”

Section: Discussionmentioning

confidence: 99%

Clonal Relationships among Shigella Serotypes Suggested by Cryptic Flagellin Gene Polymorphism

et al. 2001

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The presence of cryptic fliC alleles in the genomes of 120 strains representative of the four Shigella species was investigated. One fragment was obtained by PCR amplification of fliC, with a size varying from 1.2 to 3.2 kbp, depending on the species or serotype. After digestion with endonuclease HhaI, the number of fragments in patterns varied from three to nine, with sizes of between 115 and 1,020 bp. Patterns sharing most of their bands were grouped to constitute an F type. A total of 17 different F types were obtained from all strains included in this study. A unique pattern was observed for each the following serotypes: Shigella dysenteriae 1, 2, 8, and 10 and S. boydii 7, 13, 15, 16, and 17. On the contrary, S. dysenteriae serotype 13 and S. sonnei biotype e were each subdivided into two different F types. S. flexneri serotypes 3a and X could be distinguished from the cluster containing S. flexneri serotypes 1 to 5 and Y. S. flexneri serotype 6 clustered with S. boydii serotypes 1, 2, 3, 4, 6, 8, 10, 11, 14, and 18 and S. dysenteriae serotypes 4, 5, 6, 7, 9, 11, and 12. Two other clusters were outlined: one comprising S. dysenteriae serotypes 3, 12, 13 (strain CDC598-77), 14, and 15 and the other one joining S. boydii serotypes 5 and 9. None of the 17 fliC patterns was found in the fliC HhaI pattern database previously described for Escherichia coli. Overall, this work supports the hypothesis that Shigella evolved from different ancestral strains of E. coli. Moreover, the method outlined here is a promising tool for the identification of some clinically important Shigella strains as well as for confirmation of atypical isolates as Shigella spp.Shigella species and serotypes, except for Shigella boydii serotype 13, show more than 73% DNA relatedness to Escherichia coli K-12 (4

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“…The remaining strains of this latter species constituted a class with the S . flexneri serotype 6 strains which were clearly separated from S. flexneri serotypes 1 to 5 (Dodd & Jones, 1982;Goullet, 1980b;Goullet et al, 1983;Goullet & Picard, 1987 ;Petrovskaya & Bondarenko, 1977;Timakov et al, 1972). E. coli strains were distributed in the classes containing S. sonnei, S.JEexneri and S. dysenteriae.…”

Section: Multivariate Statistical Analysis Of the Six Groups Of Speciesmentioning

confidence: 99%

Characterization of Enterobacteria by Esterase Specific-Activity Profiles

Goullet

Picard

1990

Journal of General Microbiology

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The spectrum of specific activities and the electrophoretic mobilities of esterases produced by 550 strains of Enterobacteriaceae belonging to 36 species and subclassified into six groups (group 1, Escherichia cofi, Shi'geflu and Escherichia hermanii; group 2, genus Sizfmoneffa and genus Citrobacter; group 3, genus Kfebsieffa and genus Enterobacter; group 4, genus Serrutiu and Serratia fonticolu; group 5, genus Proteus, genus Protridenct'a and genus Motgunelfa; and group 6, genus Yersiniu) were analysed by acrylamide/agarose gel electrophoresis using standardized methods for staining and mobility comparisons. Nineteen types of esterase were defined by their respective esterase specific-activity profile (ESAP). A multiple correspondence analysis (MCA) of the ESAP data enabled 82% of the strains in the 36 species to be correctly classified. In each group, the species were clearly delineated after MCA on both ESAP and electrophoretic mobility data. In addition, the smallest number of characters pro9ding species identification of Yersinia strains by esterase polymorphism was identified by means of a binary segmentation tree technique.

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Genetic Data Concerning Shigella flexneri Serotypes 5 and 6

Cited by 11 publications

References 5 publications

A Numerical Taxonomic Study of the Genus Shigella

A Numerical Taxonomic Study of the Genus Shigella

Clonal Relationships among Shigella Serotypes Suggested by Cryptic Flagellin Gene Polymorphism

Characterization of Enterobacteria by Esterase Specific-Activity Profiles

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