Genetic data provided by 21 autosomal STR loci from Chinese Tujia ethnic group

Abstract

The aim of this study was to investigate allelic frequency distribution and forensic genetic parameters of autosomal short tandem repeats (STR) loci of the population samples from 107 Tujia individuals from Chinese Hubei Province. Twenty-one autosomal STR genetic markers (D9S1122, D6S474, D6S1017, D5S2500, D4S2408, D3S4529, D2S441, D2S1776, D22S1045, D20S482, D1S1677, D1S1627, D1GATA113, D19S433, D18S853, D17S1301, D11S4463, D12ATA63, D10S1248, D10S1435 and D14S1434) were simultaneously amplified in a new multiplex polymerase chain reaction system. 155 alleles for all the STR loci from the Tujia population were observed and the corresponding allelic frequencies ranged from 0.005 to 0.589. Expected heterozygosity, polymorphic information content, power of discrimination and power of exclusion of the 21 STR loci in the Tujia population were from 0.579 to 0.824, from 0.525 to 0.802, from 0.773 to 0.945 and from 0.257 to 0.641, respectively. Our results indicate that the autosomal STRs multiplex system provides highly informative STR data and could be useful in forensic individual identification and parentage testing in this region.

A 30-InDel Assay for Genetic Variation and Population Structure Analysis of Chinese Tujia Group

“…The lowest HO, HE, PIC, TPI, PD and PE were observed at HLD 118 locus, and this locus was also found with the lowest polymorphism in other previously studied groups10. The combined power of exclusion (CPE) and discrimination (CPD) at the 30 InDel loci in the Tujia group were 0.9860 and 0.9999999999761, respectively; combined matching probability (CMP) value of 30 InDels in the group was 2.3894 × 10 −11 , higher than that in our previous study which reached 1.10974 × 10 −19 of 21 autosomal STRs in Tujia group11. According to our calculation, the value of CMP combining 30 InDels with 21 autosomal STRs reached 2.652 × 10 −30 .…”

“…However, there were some potential limitations of STRs in forensic applications because of its relatively high mutation rate, long amplicon size, and the deficiency in the analysis of highly degraded DNA samples and complex kinship cases. In recent years, a novel genetic marker: insertion and deletion polymorphisms (InDels) dispersing through the human genome showed some advantages, such as short amplicon size, low mutation rate, and practicability of being genotyped in the present forensic DNA lab platforms678, which were useful for forensic DNA applications (Supplementary method for STR applications), population genetics9101112, and biogeographic ancestry analysis131415. Population genetic and forensic validation studies have been performed using the Qiagen Investigator DIPplex ® reagent including 30 autosomal InDel loci plus amelogenin locus, and population data of Chinese Han, Tibetan, Uigur, Kazak, She, Xibe and Yi populations have been reported in previous studies910161718.…”

Allelic frequency distributions of 21 non-combined DNA index system STR loci in a Russian ethnic minority group from Inner Mongolia, China

“…Differences were found between the Tujia (Yuan et al, 2012) and our study population at five loci (D14S1434, D20S482, D22S1045, D2S441, and D6S474). Between the Chinese Han population in Beijing (Guo et al, 2010) and our study population, differences were found at six loci (D10S1248, D10S1435, D19S433, D1GATA113, D4S2408, and D9S1122).…”

Genetic polymorphisms of 21 STR loci of Goldeneye™ DNA ID 22NC kit in five ethnic groups of China

“…F st values of Guangdong Han-Kazak, Bai-Mongolian, Bai-Hui, Bai-Tibetan, Bai-Uygur, Yi-Hui, Yi-Mongolian, Yi-Tibetan, Yi-Uygur, Salar-Mongolian, Kazak-Mongolian, Kazak-Hui, Kazak-Tibetan, were all above 0.15, showing great genetic differentiation. We also found that Inner Mongolia Mongolian, Ningxia Hui, Xinjiang Uygur, and Xizang Tibetan show moderate genetic differentiation (F st >0.1) with nine referenced ethnic groups [6][7][8][9][10][11][12][13][14]. Visual results are shown in Figure S2.…”

“…To estimate the genetic relationship between the five studied ethnic groups and other ethnic groups, available allele frequencies data of 6914 Han Chinese individuals (Guangxi Han [6], Guangdong Han [7], Hebei Han [8], Hunan Han [9]) and 1 715 minority individuals (Tujia [10], Bai [11], Yi [12], Salar [13], Kazak [14]) were referred. The detailed data source is listed in Table S1.…”