Genetic deletion of mast cell serotonin synthesis prevents the development of obesity and insulin resistance

Yabut, Julian M.; Desjardins, Eric M.; Chan, Eric J.; Day, Emily; Leroux, Julie M.; Wang, Bo; Crane, Elizabeth D.; Wong, Wesley; Morrison, Katherine M.; Crane, Justin D.; Khan, Waliul I.; Steinberg, Gregory R.

doi:10.1038/s41467-019-14080-7

Cited by 45 publications

(34 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3d ). We then pretreated ovules with the Ca 2+ ionophore A23187 (10 μM), which has been widely used to raise internal Ca 2+ levels 38 and has been convincingly shown to work in plant cells 39 – 41 . Notably, this treatment was sufficient to rescue the retention of LURE1.2-eGFP in the synergid cells of the acs octuple mutant ovules; we observed less LURE1.2-eGFP in the synergid cells and more in the filiform apparatus (Fig.…”

Section: Resultsmentioning

confidence: 99%

Ethylene-independent signaling by the ethylene precursor ACC in Arabidopsis ovular pollen tube attraction

et al. 2020

View full text Add to dashboard Cite

The phytohormone ethylene has numerous effects on plant growth and development. Its immediate precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), is a non-proteinogenic amino acid produced by ACC SYNTHASE (ACS). ACC is often used to induce ethylene responses. Here, we demonstrate that ACC exhibits ethylene-independent signaling in Arabidopsis thaliana reproduction. By analyzing an acs octuple mutant with reduced seed set, we find that ACC signaling in ovular sporophytic tissue is involved in pollen tube attraction, and promotes secretion of the pollen tube chemoattractant LURE1.2. ACC activates Ca 2+-containing ion currents via GLUTAMATE RECEPTOR-LIKE (GLR) channels in root protoplasts. In COS-7 cells expressing moss PpGLR1, ACC induces the highest cytosolic Ca 2+ elevation compared to all twenty proteinogenic amino acids. In ovules, ACC stimulates transient Ca 2+ elevation, and Ca 2+ influx in octuple mutant ovules rescues LURE1.2 secretion. These findings uncover a novel ACC function and provide insights for unraveling new physiological implications of ACC in plants.

show abstract

Section: Resultsmentioning

confidence: 99%

Ethylene-independent signaling by the ethylene precursor ACC in Arabidopsis ovular pollen tube attraction

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Furthermore, the exposure of mice to thermoneutrality promotes the infiltration of WAT with MCs that are highly enriched with Tph1. Engraftment of MC-deficient mice with Tph1 -/-MCs or selective MCs deletion of Tph1 enhances uncoupling protein 1 expression in WAT and protects mice from developing obesity and insulin resistance [48].…”

Section: Type 2 Diabetes Mellitusmentioning

confidence: 99%

Mast Cells in Diabetes and Diabetic Wound Healing

et al. 2020

View full text Add to dashboard Cite

Mast cells (MCs) are granulated, immune cells of the myeloid lineage that are present in connective tissues. Apart from their classical role in allergies, MCs also mediate various inflammatory responses due to the nature of their secretory products. They are involved in important physiological and pathophysiological responses related to inflammation, chronic wounds, and autoimmune diseases. There are also indications that MCs are associated with diabetes and its complications. MCs and MC-derived mediators participate in all wound healing stages and are involved in the pathogenesis of non-healing, chronic diabetic foot ulcers (DFUs). More specifically, recent work has shown increased degranulation of skin MCs in human diabetes and diabetic mice, which is associated with impaired wound healing. Furthermore, MC stabilization, either systemic or local at the skin level, improves wound healing in diabetic mice. Understanding the precise role of MCs in wound progression and healing processes can be of critical importance as it can lead to the development of new targeted therapies for diabetic foot ulceration, one of the most devastating complications of diabetes.

show abstract

“…Since mouse adrenal mast cells are activated by sodium restriction, it would be relevant to evaluate whether the 5-HT synthesizing enzyme Tph1 is dif-ferentially expressed in adrenal from mice under normal and low sodium diets. The impact of sodium depletion or pharmacological inhibition of RAS on 5-HT release by adrenal mast cells might also be indirectly estimated by examining aldosterone production in mice with selective Tph1 deficiency in mast cells (Kit Tph1-/mouse model [60]) in comparison with wild type or Kit W-sh/W-sh animals.…”

Section: Serotonin Secreted By Mast Cells Stimulates Aldosterone Syntmentioning

confidence: 99%

Role of Mast Cells in the Control of Aldosterone Secretion

et al. 2020

View full text Add to dashboard Cite

Mast cells are immune cells present in adrenals from various species. Proliferation and activation of adrenal mast cells seem to be influenced by environment, since they increase during summer and in response to sodium restriction in frogs and mouse, respectively. Although the physiological factors regulating adrenal mast cell activity have not been identified, they might involve neurotransmitters and the renin-angiotensin system. Some data indicate that adrenal mast cells stimulate proliferation of steroidogenic cells in the zona glomerulosa and activate the mineralocorticoid production. In human, mast cell degranulation stimulates aldosterone synthesis through the release of serotonin (5-HT) and activation of 5-HT4 receptors. Increase in mast cell population and upregulation of the 5-HT signaling pathway occur in aldosterone-producing adenomas. In particular, aldosterone-producing adenoma cells overexpress 5-HT4 receptors and are hyper-responsive to 5-HT4 receptor agonists. These data suggest that the intra-adrenal serotonergic regulatory system represents a potential target for development of both adrenal imaging methods to evaluate the lateralization of aldosterone production, and pharmacological treatments of primary aldosteronism.

show abstract

Genetic deletion of mast cell serotonin synthesis prevents the development of obesity and insulin resistance

Cited by 45 publications

References 57 publications

Ethylene-independent signaling by the ethylene precursor ACC in Arabidopsis ovular pollen tube attraction

Ethylene-independent signaling by the ethylene precursor ACC in Arabidopsis ovular pollen tube attraction

Mast Cells in Diabetes and Diabetic Wound Healing

Role of Mast Cells in the Control of Aldosterone Secretion

Contact Info

Product

Resources

About