Genetic detection to Aeromonas hydrophila proteolytic activity in milk samples (cows, buffaloes and goats) in Basra governorate

Abstract

This study aim is to determine the incidence and the virulence of Aeromonas hydrophila in raw milk, randomly collected from Basra governorate by using of polymerase chain reaction (PCR) technique. In this study, the total number of raw milk samples collected from cows, buffaloes and goats that kept from different the regions of Basra governorate were 90 samples. The PCR technique is modern method which regarded as a reliable tool to detect virulent gene of the A. hydrophila isolates. The PCR assays using the primers sets SerAh-F and SerAh-R resulted in the amplification of 650-bp bands from the targeted proteases gene of the A. hydrophil. The result of the present study showed that the results of PCR concerning the proteolytic activity of A. hydrophila in the tested raw milk samples according to animals' source. The higher percentage of the proteolytic activity was found in the cow's raw milk samples 40% and in the buffalo's milk samples was 26.7% while, the proteolytic activity did not find in the goat's milk samples. The association between the source of the milk sample and proteolytic A. hydrophila positive results was considered to be statistically highly significant. The higher percentage of the A. hydrophila isolates found in the raw cow milk was 40%, and the A. hydrophila isolates found in the raw buffalo milk was 26.7%, while, the A. hydrophila isolates did not find in the goat milk.

Serological and molecular investigations of Brucellosis in dairy cows at certain areas of Al-Sulaymaniyah governorate- Iraq

“…The application of PCR-based assays for detection and identification of Brucella species has been increased due to their accuracy, sensitivity, speed and ability to work with DNA rather than the highly infectious live cultures (7). The PCR assays were proved to be a good means for rapid and accurate diagnosis especially for slow growing bacteria like Brucella (18), Mycobacterium paratuberculosis (19), Shigella (20), lactic acid bacteria (21), and the most proteolytic active bacteria as Aeromonas hydrophila (22) and to detect Brucella DNA from clinical specimens, thus the DNA detection of pathogenic organisms have been rendered biologically safe and reducing the risk of infection of laboratory workers (14).…”

Some physiological and biochemical criteria in the local buffalo infected with stomach and intestinal worms in the city of Samarra

“…The buffalo is infected with many parasitic worms; most important are the worms of the stomach and intestines. The buffalo is exposed to infection with these worms by high ratio due to the nature of its tendency to grazing and swamping in marshes, mud areas and rivers, where the presence of larvae is very common (3), and since the infection of Buffalo by parasitic worms effects on its health and its production, so many researchers around the world and extensively interested in the epidemiology of worms that attack the buffalo and viewed the various factors that play an important role in influencing the validity and distribution of the seasons of the year and the impact of these factors in different phases of cycles Such as eggs and larval stages, as well as environmental factors affecting adult phases and the adaptation of worms to those factors (4). because of the important of Buffalo That infected with stomach and intestine worms and necessity of treatment; Several studies have been carried out on various types of treatments to show their effect on worms in buffalo, including those in Australia, India, where researchers have used many worm repellents and studied their effect on various types of gastrointestinal worms and their resistance (5).…”

Genetic diversity and clonal relatedness of Aeromonas hydrophila strains isolated from hemorrhagic septicemia’s cases in common Carp (Cyprinus carpio) farms

“…Nearly every aquatic ecosystem has had aeromonads, including chlorinated drinking water, untreated sewage, groundwater and contaminated and unpolluted rivers (1,2). In addition, various domesticated animals may also have a role for the transmission of these bacteria through animal excretion (3). As a gastrointestinal and extra intestinal infection agent in humans, Aeromonas hydrophila was associated.…”